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The relaxant effect induced by Allium sativum L. bulb aqueous extract on rat isolated trachea.

Fehri B, Ahmed MK, Aiache JM - Pharmacogn Mag (2011)

Bottom Line: Garlic is also known for its beneficial effects on the cardiovascular system.It was found that ASBAE induced a dose-dependent relaxation with recorded EC (50) values of 71.87 ± 5.90 µg/mL (n = 7).It is suggested that the recorded relaxation results are due to the release of prostaglandins E (1) and E (2) consecutively to α- and β-adrenoreceptor stimulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, Tunisia Pharmaceutical Industries Company (SIPHAT), Fondouk Choucha-Radès, 2013 Ben Arous, Tunisia.

ABSTRACT

Background: Garlic plays an important role in complementary and alternative medicine. Most people believe in and use herbal products even when they have not been as thoroughly researched as garlic. Garlic is also known for its beneficial effects on the cardiovascular system.

Materials and methods: The relaxant effect of Allium sativum L. bulb aqueous extract (ASBAE) containing 0.06%-0.10% of allicin was studied on isolated smooth muscle of trachea of rats precontracted using acetylcholine (10(-5) M).

Results: It was found that ASBAE induced a dose-dependent relaxation with recorded EC (50) values of 71.87 ± 5.90 µg/mL (n = 7). Pretreatments with mepyramine (10(-7) M), methysergide (10(-7) M), caffeine (10(-6) M), theophylline (10(-6) M), nifedipine (10(-6) M), and dipyridamole (10(-6) M) did not alter ASBAE concentration-response curves. In turn, concentration-response curves to ASBAE were significantly shifted toward right in the presence of aspirin (3.10(-3) M), indomethacin (10(-6) M), prazosin (10(-6) M), and propranolol (10(-7) M).

Conclusion: It is suggested that the recorded relaxation results are due to the release of prostaglandins E (1) and E (2) consecutively to α- and β-adrenoreceptor stimulation.

No MeSH data available.


Related in: MedlinePlus

Representative trace from acetylcholine (ACh 10−5 M) precontracted rat isolated trachea showing cumulative dose-response to Allium sativum L. aqueous extract. Concentrations (10−6 to 3.10−3) are in g/mL
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Figure 0001: Representative trace from acetylcholine (ACh 10−5 M) precontracted rat isolated trachea showing cumulative dose-response to Allium sativum L. aqueous extract. Concentrations (10−6 to 3.10−3) are in g/mL

Mentions: ASBAE exhibited a dose-dependent relaxant effect on the rat isolated trachea precontracted with ACh 10−5 M [Figure 1]. On basal tone of isolated tissue, ASBAE showed mild contractile effects at lower doses ranging from 10−6 to 10−4 g/mL, followed by relaxant effects at higher doses (from 10−3 to 3.10−3 g/mL) (data not shown).


The relaxant effect induced by Allium sativum L. bulb aqueous extract on rat isolated trachea.

Fehri B, Ahmed MK, Aiache JM - Pharmacogn Mag (2011)

Representative trace from acetylcholine (ACh 10−5 M) precontracted rat isolated trachea showing cumulative dose-response to Allium sativum L. aqueous extract. Concentrations (10−6 to 3.10−3) are in g/mL
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3065150&req=5

Figure 0001: Representative trace from acetylcholine (ACh 10−5 M) precontracted rat isolated trachea showing cumulative dose-response to Allium sativum L. aqueous extract. Concentrations (10−6 to 3.10−3) are in g/mL
Mentions: ASBAE exhibited a dose-dependent relaxant effect on the rat isolated trachea precontracted with ACh 10−5 M [Figure 1]. On basal tone of isolated tissue, ASBAE showed mild contractile effects at lower doses ranging from 10−6 to 10−4 g/mL, followed by relaxant effects at higher doses (from 10−3 to 3.10−3 g/mL) (data not shown).

Bottom Line: Garlic is also known for its beneficial effects on the cardiovascular system.It was found that ASBAE induced a dose-dependent relaxation with recorded EC (50) values of 71.87 ± 5.90 µg/mL (n = 7).It is suggested that the recorded relaxation results are due to the release of prostaglandins E (1) and E (2) consecutively to α- and β-adrenoreceptor stimulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, Tunisia Pharmaceutical Industries Company (SIPHAT), Fondouk Choucha-Radès, 2013 Ben Arous, Tunisia.

ABSTRACT

Background: Garlic plays an important role in complementary and alternative medicine. Most people believe in and use herbal products even when they have not been as thoroughly researched as garlic. Garlic is also known for its beneficial effects on the cardiovascular system.

Materials and methods: The relaxant effect of Allium sativum L. bulb aqueous extract (ASBAE) containing 0.06%-0.10% of allicin was studied on isolated smooth muscle of trachea of rats precontracted using acetylcholine (10(-5) M).

Results: It was found that ASBAE induced a dose-dependent relaxation with recorded EC (50) values of 71.87 ± 5.90 µg/mL (n = 7). Pretreatments with mepyramine (10(-7) M), methysergide (10(-7) M), caffeine (10(-6) M), theophylline (10(-6) M), nifedipine (10(-6) M), and dipyridamole (10(-6) M) did not alter ASBAE concentration-response curves. In turn, concentration-response curves to ASBAE were significantly shifted toward right in the presence of aspirin (3.10(-3) M), indomethacin (10(-6) M), prazosin (10(-6) M), and propranolol (10(-7) M).

Conclusion: It is suggested that the recorded relaxation results are due to the release of prostaglandins E (1) and E (2) consecutively to α- and β-adrenoreceptor stimulation.

No MeSH data available.


Related in: MedlinePlus