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Genomic revelations of a mutualism: the pea aphid and its obligate bacterial symbiont.

Shigenobu S, Wilson AC - Cell. Mol. Life Sci. (2011)

Bottom Line: Here we present a refined picture of this symbiosis by linking pre-genomic observations to new genomic data that includes the complete genomes of the eukaryotic and prokaryotic symbiotic partners.In doing so, we address four issues central to understanding the patterns and processes operating at the A. pisum/Buchnera APS interface.These four issues include: (1) lateral gene transfer, (2) host immunity, (3) symbiotic metabolism, and (4) regulation.

View Article: PubMed Central - PubMed

Affiliation: NIBB Core Research Facilities, National Institute for Basic Biology, Myodaiji, Okazaki, Japan. shige@nibb.ac.jp

ABSTRACT
The symbiosis of the pea aphid Acyrthosphion pisum with the bacterium Buchnera aphidicola APS represents the best-studied insect obligate symbiosis. Here we present a refined picture of this symbiosis by linking pre-genomic observations to new genomic data that includes the complete genomes of the eukaryotic and prokaryotic symbiotic partners. In doing so, we address four issues central to understanding the patterns and processes operating at the A. pisum/Buchnera APS interface. These four issues include: (1) lateral gene transfer, (2) host immunity, (3) symbiotic metabolism, and (4) regulation.

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Results of Mittler [34] single amino acid elimination experiment demonstrating Buchnera provisioning of essential amino acids. Nonessential amino acids (listed alphabetically) are represented by blue bars, while essential amino acids (also alphabetically listed) are shown in pink. Dark colors show data from symbiotic Myzus persicae while light colors show data from aposymbiotic M. persicae. While the data are largely qualitative, they are representative of the types of early data that established the nutritional provisioning of essential amino acids by Buchnera to their aphid hosts. Data are taken from 3day/apterous columns in Table 2 of [34]
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Fig2: Results of Mittler [34] single amino acid elimination experiment demonstrating Buchnera provisioning of essential amino acids. Nonessential amino acids (listed alphabetically) are represented by blue bars, while essential amino acids (also alphabetically listed) are shown in pink. Dark colors show data from symbiotic Myzus persicae while light colors show data from aposymbiotic M. persicae. While the data are largely qualitative, they are representative of the types of early data that established the nutritional provisioning of essential amino acids by Buchnera to their aphid hosts. Data are taken from 3day/apterous columns in Table 2 of [34]

Mentions: The nutritional basis of the aphid/Buchnera symbiosis was postulated almost 60 years ago by Buchner [30], tested using artificial diet assays and generation of aposymbiotic aphids in the 1960s–1990s (e.g., see Fig. 2 generated from [34–36]) and finally irrevocably confirmed in the past decade with whole genome sequencing of multiple lineages of Buchnera and most recently the pea aphid, A. pisum [9, 10, 37–39]. While the nutritional basis of the aphid/Buchnera symbioses is now well appreciated, the regulatory processes and structures that mediate the intimate interactions between aphids and Buchnera have largely been neglected. In most part this neglect stems from the fact that until now the aphid model has been insufficiently equipped with the genetic and molecular tools necessary for characterization of regulatory processes and structures operating at the symbiotic interface. That said, the last 40+ years of research have left us with a comprehensive blueprint of the amino acid metabolism of this symbiosis, a blueprint that provides the foundation for hypothesis-testing research focused on characterization of the regulatory processes and structures operating at the symbiotic interface.Fig. 2


Genomic revelations of a mutualism: the pea aphid and its obligate bacterial symbiont.

Shigenobu S, Wilson AC - Cell. Mol. Life Sci. (2011)

Results of Mittler [34] single amino acid elimination experiment demonstrating Buchnera provisioning of essential amino acids. Nonessential amino acids (listed alphabetically) are represented by blue bars, while essential amino acids (also alphabetically listed) are shown in pink. Dark colors show data from symbiotic Myzus persicae while light colors show data from aposymbiotic M. persicae. While the data are largely qualitative, they are representative of the types of early data that established the nutritional provisioning of essential amino acids by Buchnera to their aphid hosts. Data are taken from 3day/apterous columns in Table 2 of [34]
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3064905&req=5

Fig2: Results of Mittler [34] single amino acid elimination experiment demonstrating Buchnera provisioning of essential amino acids. Nonessential amino acids (listed alphabetically) are represented by blue bars, while essential amino acids (also alphabetically listed) are shown in pink. Dark colors show data from symbiotic Myzus persicae while light colors show data from aposymbiotic M. persicae. While the data are largely qualitative, they are representative of the types of early data that established the nutritional provisioning of essential amino acids by Buchnera to their aphid hosts. Data are taken from 3day/apterous columns in Table 2 of [34]
Mentions: The nutritional basis of the aphid/Buchnera symbiosis was postulated almost 60 years ago by Buchner [30], tested using artificial diet assays and generation of aposymbiotic aphids in the 1960s–1990s (e.g., see Fig. 2 generated from [34–36]) and finally irrevocably confirmed in the past decade with whole genome sequencing of multiple lineages of Buchnera and most recently the pea aphid, A. pisum [9, 10, 37–39]. While the nutritional basis of the aphid/Buchnera symbioses is now well appreciated, the regulatory processes and structures that mediate the intimate interactions between aphids and Buchnera have largely been neglected. In most part this neglect stems from the fact that until now the aphid model has been insufficiently equipped with the genetic and molecular tools necessary for characterization of regulatory processes and structures operating at the symbiotic interface. That said, the last 40+ years of research have left us with a comprehensive blueprint of the amino acid metabolism of this symbiosis, a blueprint that provides the foundation for hypothesis-testing research focused on characterization of the regulatory processes and structures operating at the symbiotic interface.Fig. 2

Bottom Line: Here we present a refined picture of this symbiosis by linking pre-genomic observations to new genomic data that includes the complete genomes of the eukaryotic and prokaryotic symbiotic partners.In doing so, we address four issues central to understanding the patterns and processes operating at the A. pisum/Buchnera APS interface.These four issues include: (1) lateral gene transfer, (2) host immunity, (3) symbiotic metabolism, and (4) regulation.

View Article: PubMed Central - PubMed

Affiliation: NIBB Core Research Facilities, National Institute for Basic Biology, Myodaiji, Okazaki, Japan. shige@nibb.ac.jp

ABSTRACT
The symbiosis of the pea aphid Acyrthosphion pisum with the bacterium Buchnera aphidicola APS represents the best-studied insect obligate symbiosis. Here we present a refined picture of this symbiosis by linking pre-genomic observations to new genomic data that includes the complete genomes of the eukaryotic and prokaryotic symbiotic partners. In doing so, we address four issues central to understanding the patterns and processes operating at the A. pisum/Buchnera APS interface. These four issues include: (1) lateral gene transfer, (2) host immunity, (3) symbiotic metabolism, and (4) regulation.

Show MeSH