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Integrative analysis of intraerythrocytic differentially expressed transcripts yields novel insights into the biology of Plasmodium falciparum.

Isokpehi RD, Hide WA - Malar. J. (2003)

Bottom Line: There has been a significant increase in disparate datasets derived from genomic and post-genomic analysis of the parasite that necessitates delivery of integrated analysis from which biological processes important to the survival of the parasite can be determined.METHODS: In order to resolve genes associated with stage differentially expressed transcripts, we have developed and implemented an integrative approach that combines evidence from P. falciparum expressed sequence tags (ESTs), genomic, microarray, proteomic and gene ontology data.A subset of 74 genes associated with these transcripts showed evidence of stage-correlated protein expression, of which 53 have not been experimentally characterised.

View Article: PubMed Central - HTML - PubMed

Affiliation: South African National Bioinformatics Institute, University of the Western Cape, Bellville 7535, South Africa. raphael@sanbi.ac.za

ABSTRACT
BACKGROUND: The intraerythrocytic development of Plasmodium falciparum, the most virulent human malaria parasite involves asexual and gametocyte stages. There has been a significant increase in disparate datasets derived from genomic and post-genomic analysis of the parasite that necessitates delivery of integrated analysis from which biological processes important to the survival of the parasite can be determined. METHODS: In order to resolve genes associated with stage differentially expressed transcripts, we have developed and implemented an integrative approach that combines evidence from P. falciparum expressed sequence tags (ESTs), genomic, microarray, proteomic and gene ontology data. RESULTS: A total of 143 gametocyte-overexpressed and 51 asexual-overexpressed transcripts were identified. A subset of 74 genes associated with these transcripts showed evidence of stage-correlated protein expression, of which 53 have not been experimentally characterised. Our study has revealed (1) possible regulatory mechanisms in malaria parasites' gametocyte maturation, (2) correlation between EST and microarray data for a P. falciparum gene family to present unique EST-derived information, (3) candidate drug and antigenic targets on which computational and experimental studies can be performed, and (4) the need for more empirical studies on gene and protein expression in malaria parasites. CONCLUSION: Applying different domains of data to the same underlying gene set has yielded novel insights into the biology of the parasite and presents an approach to appraise critically the data quality of post-genomic datasets from malaria parasites.

No MeSH data available.


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Simplified flowchart of integrative analysis of Plasmodium falciparum data. Flowchart symbols: rounded rectangle, start or end; rectangle, process; diamond, decision.
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Figure 1: Simplified flowchart of integrative analysis of Plasmodium falciparum data. Flowchart symbols: rounded rectangle, start or end; rectangle, process; diamond, decision.

Mentions: The integrative analysis approach that was used to combine genomic, expressed sequence tag, microarray, proteomic and gene ontology data from P. falciparum 3D7 is presented in Figure 1. The starting integrative criterion was significant overexpression of a transcript in a stage relative to the other stage. Criteria used and their acceptable ranges are presented in Table 1.


Integrative analysis of intraerythrocytic differentially expressed transcripts yields novel insights into the biology of Plasmodium falciparum.

Isokpehi RD, Hide WA - Malar. J. (2003)

Simplified flowchart of integrative analysis of Plasmodium falciparum data. Flowchart symbols: rounded rectangle, start or end; rectangle, process; diamond, decision.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC305352&req=5

Figure 1: Simplified flowchart of integrative analysis of Plasmodium falciparum data. Flowchart symbols: rounded rectangle, start or end; rectangle, process; diamond, decision.
Mentions: The integrative analysis approach that was used to combine genomic, expressed sequence tag, microarray, proteomic and gene ontology data from P. falciparum 3D7 is presented in Figure 1. The starting integrative criterion was significant overexpression of a transcript in a stage relative to the other stage. Criteria used and their acceptable ranges are presented in Table 1.

Bottom Line: There has been a significant increase in disparate datasets derived from genomic and post-genomic analysis of the parasite that necessitates delivery of integrated analysis from which biological processes important to the survival of the parasite can be determined.METHODS: In order to resolve genes associated with stage differentially expressed transcripts, we have developed and implemented an integrative approach that combines evidence from P. falciparum expressed sequence tags (ESTs), genomic, microarray, proteomic and gene ontology data.A subset of 74 genes associated with these transcripts showed evidence of stage-correlated protein expression, of which 53 have not been experimentally characterised.

View Article: PubMed Central - HTML - PubMed

Affiliation: South African National Bioinformatics Institute, University of the Western Cape, Bellville 7535, South Africa. raphael@sanbi.ac.za

ABSTRACT
BACKGROUND: The intraerythrocytic development of Plasmodium falciparum, the most virulent human malaria parasite involves asexual and gametocyte stages. There has been a significant increase in disparate datasets derived from genomic and post-genomic analysis of the parasite that necessitates delivery of integrated analysis from which biological processes important to the survival of the parasite can be determined. METHODS: In order to resolve genes associated with stage differentially expressed transcripts, we have developed and implemented an integrative approach that combines evidence from P. falciparum expressed sequence tags (ESTs), genomic, microarray, proteomic and gene ontology data. RESULTS: A total of 143 gametocyte-overexpressed and 51 asexual-overexpressed transcripts were identified. A subset of 74 genes associated with these transcripts showed evidence of stage-correlated protein expression, of which 53 have not been experimentally characterised. Our study has revealed (1) possible regulatory mechanisms in malaria parasites' gametocyte maturation, (2) correlation between EST and microarray data for a P. falciparum gene family to present unique EST-derived information, (3) candidate drug and antigenic targets on which computational and experimental studies can be performed, and (4) the need for more empirical studies on gene and protein expression in malaria parasites. CONCLUSION: Applying different domains of data to the same underlying gene set has yielded novel insights into the biology of the parasite and presents an approach to appraise critically the data quality of post-genomic datasets from malaria parasites.

No MeSH data available.


Related in: MedlinePlus