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Southeast Asian diversity: first insights into the complex mtDNA structure of Laos.

Bodner M, Zimmermann B, Röck A, Kloss-Brandstätter A, Horst D, Horst B, Sengchanh S, Sanguansermsri T, Horst J, Krämer T, Schneider PM, Parson W - BMC Evol. Biol. (2011)

Bottom Line: We found several new, so far undescribed mtDNA lineages in this dataset and surrounding populations.The Laos population was characteristic in terms of haplotype composition and genetic structure, however, genetic comparisons with other Southeast Asian populations revealed limited, but significant genetic differentiation.They encourage setting up regional and subpopulation databases, especially for forensic applications.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Legal Medicine, Innsbruck Medical University, Austria.

ABSTRACT

Background: Vast migrations and subsequent assimilation processes have shaped the genetic composition of Southeast Asia, an area of close contact between several major ethnic groups. To better characterize the genetic variation of this region, we analyzed the entire mtDNA control region of 214 unrelated donors from Laos according to highest forensic quality standards. To detail the phylogeny, we inspected selected SNPs from the mtDNA coding region. For a posteriori data quality control, quasi-median network constructions and autosomal STR typing were performed. In order to describe the mtDNA setup of Laos more thoroughly, the data were subjected to population genetic comparisons with 16 East Asian groups.

Results: The Laos sample exhibited ample mtDNA diversity, reflecting the huge number of ethnic groups listed. We found several new, so far undescribed mtDNA lineages in this dataset and surrounding populations. The Laos population was characteristic in terms of haplotype composition and genetic structure, however, genetic comparisons with other Southeast Asian populations revealed limited, but significant genetic differentiation. Notable differences in the maternal relationship to the major indigenous Southeast Asian ethnolinguistic groups were detected.

Conclusions: In this study, we portray the great mtDNA variety of Laos for the first time. Our findings will contribute to clarify the migration history of the region. They encourage setting up regional and subpopulation databases, especially for forensic applications. The Laotian sequences will be incorporated into the collaborative EMPOP mtDNA database http://www.empop.org upon publication and will be available as the first mtDNA reference data for this country.

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Multidimensional scaling analysis of genetic distances between 17 population samples from East Asia. Computed from FST indices based on nps 16024-16383 (see Additional file 8). Stress value: 0.085. Abbreviations: AA - Austro-Asiatic, ISEA - Island Southeast Asians, SIM - Southern Indigenous Minorities, STB - Southern Tibeto-Burmans. References: [3-7,12,17,23-26].
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Figure 4: Multidimensional scaling analysis of genetic distances between 17 population samples from East Asia. Computed from FST indices based on nps 16024-16383 (see Additional file 8). Stress value: 0.085. Abbreviations: AA - Austro-Asiatic, ISEA - Island Southeast Asians, SIM - Southern Indigenous Minorities, STB - Southern Tibeto-Burmans. References: [3-7,12,17,23-26].

Mentions: An MDS plot for visualization of the AMOVA results is depicted in Figure 4. The positioning of the samples did not change when nonsignificant FST values (see Additional file 8) were excluded. In this correspondence analysis, the population sample from Laos clustered with the samples from Vietnam [23] and Thailand [17] and the Southern Indigenous Minorities [12]. This might be explained by the fact that these four samples do not represent ethnic entities, but are a similar combination of several groups. The partly shared political history of the three neighboring countries could have facilitated migrations.


Southeast Asian diversity: first insights into the complex mtDNA structure of Laos.

Bodner M, Zimmermann B, Röck A, Kloss-Brandstätter A, Horst D, Horst B, Sengchanh S, Sanguansermsri T, Horst J, Krämer T, Schneider PM, Parson W - BMC Evol. Biol. (2011)

Multidimensional scaling analysis of genetic distances between 17 population samples from East Asia. Computed from FST indices based on nps 16024-16383 (see Additional file 8). Stress value: 0.085. Abbreviations: AA - Austro-Asiatic, ISEA - Island Southeast Asians, SIM - Southern Indigenous Minorities, STB - Southern Tibeto-Burmans. References: [3-7,12,17,23-26].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3050724&req=5

Figure 4: Multidimensional scaling analysis of genetic distances between 17 population samples from East Asia. Computed from FST indices based on nps 16024-16383 (see Additional file 8). Stress value: 0.085. Abbreviations: AA - Austro-Asiatic, ISEA - Island Southeast Asians, SIM - Southern Indigenous Minorities, STB - Southern Tibeto-Burmans. References: [3-7,12,17,23-26].
Mentions: An MDS plot for visualization of the AMOVA results is depicted in Figure 4. The positioning of the samples did not change when nonsignificant FST values (see Additional file 8) were excluded. In this correspondence analysis, the population sample from Laos clustered with the samples from Vietnam [23] and Thailand [17] and the Southern Indigenous Minorities [12]. This might be explained by the fact that these four samples do not represent ethnic entities, but are a similar combination of several groups. The partly shared political history of the three neighboring countries could have facilitated migrations.

Bottom Line: We found several new, so far undescribed mtDNA lineages in this dataset and surrounding populations.The Laos population was characteristic in terms of haplotype composition and genetic structure, however, genetic comparisons with other Southeast Asian populations revealed limited, but significant genetic differentiation.They encourage setting up regional and subpopulation databases, especially for forensic applications.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Legal Medicine, Innsbruck Medical University, Austria.

ABSTRACT

Background: Vast migrations and subsequent assimilation processes have shaped the genetic composition of Southeast Asia, an area of close contact between several major ethnic groups. To better characterize the genetic variation of this region, we analyzed the entire mtDNA control region of 214 unrelated donors from Laos according to highest forensic quality standards. To detail the phylogeny, we inspected selected SNPs from the mtDNA coding region. For a posteriori data quality control, quasi-median network constructions and autosomal STR typing were performed. In order to describe the mtDNA setup of Laos more thoroughly, the data were subjected to population genetic comparisons with 16 East Asian groups.

Results: The Laos sample exhibited ample mtDNA diversity, reflecting the huge number of ethnic groups listed. We found several new, so far undescribed mtDNA lineages in this dataset and surrounding populations. The Laos population was characteristic in terms of haplotype composition and genetic structure, however, genetic comparisons with other Southeast Asian populations revealed limited, but significant genetic differentiation. Notable differences in the maternal relationship to the major indigenous Southeast Asian ethnolinguistic groups were detected.

Conclusions: In this study, we portray the great mtDNA variety of Laos for the first time. Our findings will contribute to clarify the migration history of the region. They encourage setting up regional and subpopulation databases, especially for forensic applications. The Laotian sequences will be incorporated into the collaborative EMPOP mtDNA database http://www.empop.org upon publication and will be available as the first mtDNA reference data for this country.

Show MeSH