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Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment.

Steukers L, Vandekerckhove AP, Van den Broeck W, Glorieux S, Nauwynck HJ - Vet. Res. (2011)

Bottom Line: All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi.However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes.These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. lennert.steukers@ugent.be.

ABSTRACT
In general, members of the Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. Bovine herpesvirus type 1 (BoHV-1) may replicate at both sites and cause two major clinical entities designated as infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa whereas subtype 1.2 favors replication at the peripheral genital tract. However, some studies are in contrast with this hypothesis. A thorough study of primary replication at both mucosae could elucidate whether or not different BoHV-1 subtypes show differences in mucosa tropism. We established bovine respiratory and genital organ cultures with emphasis on maintenance of tissue morphology and viability during in vitro culture. In a next step, bovine respiratory and genital mucosa explants of the same animals were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral invasion in the mucosa was performed at 0 h, 24 h, 48 h and 72 h post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane. All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi. However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes. These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.

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Replication characteristics of different BoHV-1 subtypes in respiratory and genital tissues derived from the same animals. Evolution of plaque latitude (a) and plaque penetration depth underneath the basement membrane (BM) (b) was evaluated at 0 h, 24 h, 48 h and 72 h pi. Data are given as means + SD (error bars). Significant differences between respiratory and genital tissue are indicated by means of asterisks.
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Figure 6: Replication characteristics of different BoHV-1 subtypes in respiratory and genital tissues derived from the same animals. Evolution of plaque latitude (a) and plaque penetration depth underneath the basement membrane (BM) (b) was evaluated at 0 h, 24 h, 48 h and 72 h pi. Data are given as means + SD (error bars). Significant differences between respiratory and genital tissue are indicated by means of asterisks.

Mentions: Individual plaque measurement was performed at 0 h, 24 h, 48 h and 72 h pi (Figure 6a).


Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment.

Steukers L, Vandekerckhove AP, Van den Broeck W, Glorieux S, Nauwynck HJ - Vet. Res. (2011)

Replication characteristics of different BoHV-1 subtypes in respiratory and genital tissues derived from the same animals. Evolution of plaque latitude (a) and plaque penetration depth underneath the basement membrane (BM) (b) was evaluated at 0 h, 24 h, 48 h and 72 h pi. Data are given as means + SD (error bars). Significant differences between respiratory and genital tissue are indicated by means of asterisks.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3050707&req=5

Figure 6: Replication characteristics of different BoHV-1 subtypes in respiratory and genital tissues derived from the same animals. Evolution of plaque latitude (a) and plaque penetration depth underneath the basement membrane (BM) (b) was evaluated at 0 h, 24 h, 48 h and 72 h pi. Data are given as means + SD (error bars). Significant differences between respiratory and genital tissue are indicated by means of asterisks.
Mentions: Individual plaque measurement was performed at 0 h, 24 h, 48 h and 72 h pi (Figure 6a).

Bottom Line: All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi.However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes.These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. lennert.steukers@ugent.be.

ABSTRACT
In general, members of the Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. Bovine herpesvirus type 1 (BoHV-1) may replicate at both sites and cause two major clinical entities designated as infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa whereas subtype 1.2 favors replication at the peripheral genital tract. However, some studies are in contrast with this hypothesis. A thorough study of primary replication at both mucosae could elucidate whether or not different BoHV-1 subtypes show differences in mucosa tropism. We established bovine respiratory and genital organ cultures with emphasis on maintenance of tissue morphology and viability during in vitro culture. In a next step, bovine respiratory and genital mucosa explants of the same animals were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral invasion in the mucosa was performed at 0 h, 24 h, 48 h and 72 h post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane. All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi. However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes. These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.

Show MeSH
Related in: MedlinePlus