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Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment.

Steukers L, Vandekerckhove AP, Van den Broeck W, Glorieux S, Nauwynck HJ - Vet. Res. (2011)

Bottom Line: All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi.However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes.These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. lennert.steukers@ugent.be.

ABSTRACT
In general, members of the Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. Bovine herpesvirus type 1 (BoHV-1) may replicate at both sites and cause two major clinical entities designated as infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa whereas subtype 1.2 favors replication at the peripheral genital tract. However, some studies are in contrast with this hypothesis. A thorough study of primary replication at both mucosae could elucidate whether or not different BoHV-1 subtypes show differences in mucosa tropism. We established bovine respiratory and genital organ cultures with emphasis on maintenance of tissue morphology and viability during in vitro culture. In a next step, bovine respiratory and genital mucosa explants of the same animals were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral invasion in the mucosa was performed at 0 h, 24 h, 48 h and 72 h post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane. All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi. However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes. These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.

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Confocal fluorescent images of bovine respiratory (a) and genital (b) mucosa explants inoculated with different BoHV-1 subtypes at 24 h pi (left side) and 72 h pi (right side). Viral antigen is colored with an FITC®- labeled goat anti-IBR polyclonal antiserum. Collagen VII is marked with mouse anti-collagen VII and goat anti-mouse Texas Red® antibodies.
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Figure 5: Confocal fluorescent images of bovine respiratory (a) and genital (b) mucosa explants inoculated with different BoHV-1 subtypes at 24 h pi (left side) and 72 h pi (right side). Viral antigen is colored with an FITC®- labeled goat anti-IBR polyclonal antiserum. Collagen VII is marked with mouse anti-collagen VII and goat anti-mouse Texas Red® antibodies.

Mentions: Individual plaques were visible starting from 24 h pi for all strains in all tissues. All different BoHV-1 subtypes were found to spread in a plaquewise manner in both the respiratory and genital mucosa derived from three animals. The analysis of plaque latitude and penetration depth underneath the BM was performed at different time points pi for all BoHV-1 subtypes on proximal trachea and vestibulum vaginae (Figure 5).


Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment.

Steukers L, Vandekerckhove AP, Van den Broeck W, Glorieux S, Nauwynck HJ - Vet. Res. (2011)

Confocal fluorescent images of bovine respiratory (a) and genital (b) mucosa explants inoculated with different BoHV-1 subtypes at 24 h pi (left side) and 72 h pi (right side). Viral antigen is colored with an FITC®- labeled goat anti-IBR polyclonal antiserum. Collagen VII is marked with mouse anti-collagen VII and goat anti-mouse Texas Red® antibodies.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3050707&req=5

Figure 5: Confocal fluorescent images of bovine respiratory (a) and genital (b) mucosa explants inoculated with different BoHV-1 subtypes at 24 h pi (left side) and 72 h pi (right side). Viral antigen is colored with an FITC®- labeled goat anti-IBR polyclonal antiserum. Collagen VII is marked with mouse anti-collagen VII and goat anti-mouse Texas Red® antibodies.
Mentions: Individual plaques were visible starting from 24 h pi for all strains in all tissues. All different BoHV-1 subtypes were found to spread in a plaquewise manner in both the respiratory and genital mucosa derived from three animals. The analysis of plaque latitude and penetration depth underneath the BM was performed at different time points pi for all BoHV-1 subtypes on proximal trachea and vestibulum vaginae (Figure 5).

Bottom Line: All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi.However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes.These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. lennert.steukers@ugent.be.

ABSTRACT
In general, members of the Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. Bovine herpesvirus type 1 (BoHV-1) may replicate at both sites and cause two major clinical entities designated as infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa whereas subtype 1.2 favors replication at the peripheral genital tract. However, some studies are in contrast with this hypothesis. A thorough study of primary replication at both mucosae could elucidate whether or not different BoHV-1 subtypes show differences in mucosa tropism. We established bovine respiratory and genital organ cultures with emphasis on maintenance of tissue morphology and viability during in vitro culture. In a next step, bovine respiratory and genital mucosa explants of the same animals were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral invasion in the mucosa was performed at 0 h, 24 h, 48 h and 72 h post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane. All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi. However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes. These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.

Show MeSH
Related in: MedlinePlus