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Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment.

Steukers L, Vandekerckhove AP, Van den Broeck W, Glorieux S, Nauwynck HJ - Vet. Res. (2011)

Bottom Line: All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi.However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes.These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. lennert.steukers@ugent.be.

ABSTRACT
In general, members of the Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. Bovine herpesvirus type 1 (BoHV-1) may replicate at both sites and cause two major clinical entities designated as infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa whereas subtype 1.2 favors replication at the peripheral genital tract. However, some studies are in contrast with this hypothesis. A thorough study of primary replication at both mucosae could elucidate whether or not different BoHV-1 subtypes show differences in mucosa tropism. We established bovine respiratory and genital organ cultures with emphasis on maintenance of tissue morphology and viability during in vitro culture. In a next step, bovine respiratory and genital mucosa explants of the same animals were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral invasion in the mucosa was performed at 0 h, 24 h, 48 h and 72 h post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane. All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi. However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes. These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.

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Photomicrographs of bovine stratified squamous epithelium lining the vestibulum vaginae at 0 h (a) and 96 h (b) of in vitro cultivation (end of experiment) (HE-staining). One cow showed zones containing an active mucus-secreting (PAS +) stratified columnar to cuboidal epithelium, prominent at 0 h of in vitro cultivation. This active epithelium is shown in c (HE-staining) and d (PAS-staining).
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Figure 1: Photomicrographs of bovine stratified squamous epithelium lining the vestibulum vaginae at 0 h (a) and 96 h (b) of in vitro cultivation (end of experiment) (HE-staining). One cow showed zones containing an active mucus-secreting (PAS +) stratified columnar to cuboidal epithelium, prominent at 0 h of in vitro cultivation. This active epithelium is shown in c (HE-staining) and d (PAS-staining).

Mentions: No significant changes were noticed in the epithelial thickness of the vestibulum vaginae measured during cultivation. A clear non-keratinized stratified squamous epithelium was visible for all samples (Figure 1a-b). However, for all cows, a few zones containing a stratified columnar to stratified cuboidal epithelium were observed. Moreover, one cow (1.03 ng/mL P4 level) showed especially at 0 h of cultivation some columnar to cuboidal cells in the stratified columnar to cuboidal epithelial zones containing a strong periodic acid + Schiff (PAS) positive substance (Figure 1c-d).


Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment.

Steukers L, Vandekerckhove AP, Van den Broeck W, Glorieux S, Nauwynck HJ - Vet. Res. (2011)

Photomicrographs of bovine stratified squamous epithelium lining the vestibulum vaginae at 0 h (a) and 96 h (b) of in vitro cultivation (end of experiment) (HE-staining). One cow showed zones containing an active mucus-secreting (PAS +) stratified columnar to cuboidal epithelium, prominent at 0 h of in vitro cultivation. This active epithelium is shown in c (HE-staining) and d (PAS-staining).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3050707&req=5

Figure 1: Photomicrographs of bovine stratified squamous epithelium lining the vestibulum vaginae at 0 h (a) and 96 h (b) of in vitro cultivation (end of experiment) (HE-staining). One cow showed zones containing an active mucus-secreting (PAS +) stratified columnar to cuboidal epithelium, prominent at 0 h of in vitro cultivation. This active epithelium is shown in c (HE-staining) and d (PAS-staining).
Mentions: No significant changes were noticed in the epithelial thickness of the vestibulum vaginae measured during cultivation. A clear non-keratinized stratified squamous epithelium was visible for all samples (Figure 1a-b). However, for all cows, a few zones containing a stratified columnar to stratified cuboidal epithelium were observed. Moreover, one cow (1.03 ng/mL P4 level) showed especially at 0 h of cultivation some columnar to cuboidal cells in the stratified columnar to cuboidal epithelial zones containing a strong periodic acid + Schiff (PAS) positive substance (Figure 1c-d).

Bottom Line: All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi.However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes.These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. lennert.steukers@ugent.be.

ABSTRACT
In general, members of the Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. Bovine herpesvirus type 1 (BoHV-1) may replicate at both sites and cause two major clinical entities designated as infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa whereas subtype 1.2 favors replication at the peripheral genital tract. However, some studies are in contrast with this hypothesis. A thorough study of primary replication at both mucosae could elucidate whether or not different BoHV-1 subtypes show differences in mucosa tropism. We established bovine respiratory and genital organ cultures with emphasis on maintenance of tissue morphology and viability during in vitro culture. In a next step, bovine respiratory and genital mucosa explants of the same animals were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral invasion in the mucosa was performed at 0 h, 24 h, 48 h and 72 h post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane. All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi. However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes. These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.

Show MeSH
Related in: MedlinePlus