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Hepcidin and iron species distribution inside the first-trimester human gestational sac.

Evans P, Cindrova-Davies T, Muttukrishna S, Burton GJ, Porter J, Jauniaux E - Mol. Hum. Reprod. (2010)

Bottom Line: In contrast, transferrin saturations were significantly higher in fetal (77%) than maternal serum (33%).Immunostaining with Hep antibody was found in the syncytiotrophoblast of first-trimester placenta as well as in mesothelial and endodermal layers of the secondary yolk sac at 10 weeks.The low transferrin concentrations and the presence of NTBI in CF and AF suggest that transferrin-independent iron transfer is important in early gestation.

View Article: PubMed Central - PubMed

Affiliation: Department of Haematology, UCL Cancer Institute (Evans and Porter), London, UK.

ABSTRACT
We have investigated factors affecting iron distribution in the first-trimester gestational sac, by the measurement of transferrin, non-transferrin-bound iron (NTBI) and pro-hepcidin (Hep) in maternal serum, coelomic fluid (CF) and amniotic fluid (AF) and by immunostaining for Hep in villous and secondary yolk sac biopsies. These samples were obtained from 15 first-trimester pregnancies at 8-11 weeks gestation. Transferrin concentrations were significantly lower in fetal (0.56 mg/ml) than maternal serum (1.71 mg/ml), with very low concentrations in CF and AF (0.09 mg/ml). In contrast, transferrin saturations were significantly higher in fetal (77%) than maternal serum (33%). NTBI was present in fetal serum, CF and AF, presumably as a consequence of low transferrin concentrations in these compartments. Pro-Hep was present at lower levels in fetal (140.0 ± 11.1) than maternal serum (206.2 ± 9.2) and at low concentrations in CF (19.4 ± 3.1) and AF (21.8 ± 5.2). Immunostaining with Hep antibody was found in the syncytiotrophoblast of first-trimester placenta as well as in mesothelial and endodermal layers of the secondary yolk sac at 10 weeks. The presence of Hep in syncytiotrophoblast cells of first-trimester placenta as well as in mesothelial and endodermal layers of the secondary yolk sac suggest a key regulatory role for this protein in iron transfer to the first-trimester fetus. The low transferrin concentrations and the presence of NTBI in CF and AF suggest that transferrin-independent iron transfer is important in early gestation.

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Immunostaining with Hep antibody (brown) in mesothelial (A and B) and endodermal (A) layers of the secondary yolk sac at 10 week. Nuclei are stained blue (A ×25; B ×75).
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GAQ101F2: Immunostaining with Hep antibody (brown) in mesothelial (A and B) and endodermal (A) layers of the secondary yolk sac at 10 week. Nuclei are stained blue (A ×25; B ×75).

Mentions: Hep staining was also apparent in the secondary yolk sac. The antibody labelled strongly the cytoplasm of the external mesothelial layer and to much lesser extent the internal endodermal layer (Fig. 2). The cytoplasm and nuclei of the mesothelial layer stained uniformly, whereas the endodermal cytoplasm largely did not stain and none of the endodermal nuclei reacted positively. The mesenchyme, blood islands and ducts did not show labelling. Negative control sections, which were not exposed to primary antibodies, showed no staining (not shown).Figure 2


Hepcidin and iron species distribution inside the first-trimester human gestational sac.

Evans P, Cindrova-Davies T, Muttukrishna S, Burton GJ, Porter J, Jauniaux E - Mol. Hum. Reprod. (2010)

Immunostaining with Hep antibody (brown) in mesothelial (A and B) and endodermal (A) layers of the secondary yolk sac at 10 week. Nuclei are stained blue (A ×25; B ×75).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3050573&req=5

GAQ101F2: Immunostaining with Hep antibody (brown) in mesothelial (A and B) and endodermal (A) layers of the secondary yolk sac at 10 week. Nuclei are stained blue (A ×25; B ×75).
Mentions: Hep staining was also apparent in the secondary yolk sac. The antibody labelled strongly the cytoplasm of the external mesothelial layer and to much lesser extent the internal endodermal layer (Fig. 2). The cytoplasm and nuclei of the mesothelial layer stained uniformly, whereas the endodermal cytoplasm largely did not stain and none of the endodermal nuclei reacted positively. The mesenchyme, blood islands and ducts did not show labelling. Negative control sections, which were not exposed to primary antibodies, showed no staining (not shown).Figure 2

Bottom Line: In contrast, transferrin saturations were significantly higher in fetal (77%) than maternal serum (33%).Immunostaining with Hep antibody was found in the syncytiotrophoblast of first-trimester placenta as well as in mesothelial and endodermal layers of the secondary yolk sac at 10 weeks.The low transferrin concentrations and the presence of NTBI in CF and AF suggest that transferrin-independent iron transfer is important in early gestation.

View Article: PubMed Central - PubMed

Affiliation: Department of Haematology, UCL Cancer Institute (Evans and Porter), London, UK.

ABSTRACT
We have investigated factors affecting iron distribution in the first-trimester gestational sac, by the measurement of transferrin, non-transferrin-bound iron (NTBI) and pro-hepcidin (Hep) in maternal serum, coelomic fluid (CF) and amniotic fluid (AF) and by immunostaining for Hep in villous and secondary yolk sac biopsies. These samples were obtained from 15 first-trimester pregnancies at 8-11 weeks gestation. Transferrin concentrations were significantly lower in fetal (0.56 mg/ml) than maternal serum (1.71 mg/ml), with very low concentrations in CF and AF (0.09 mg/ml). In contrast, transferrin saturations were significantly higher in fetal (77%) than maternal serum (33%). NTBI was present in fetal serum, CF and AF, presumably as a consequence of low transferrin concentrations in these compartments. Pro-Hep was present at lower levels in fetal (140.0 ± 11.1) than maternal serum (206.2 ± 9.2) and at low concentrations in CF (19.4 ± 3.1) and AF (21.8 ± 5.2). Immunostaining with Hep antibody was found in the syncytiotrophoblast of first-trimester placenta as well as in mesothelial and endodermal layers of the secondary yolk sac at 10 weeks. The presence of Hep in syncytiotrophoblast cells of first-trimester placenta as well as in mesothelial and endodermal layers of the secondary yolk sac suggest a key regulatory role for this protein in iron transfer to the first-trimester fetus. The low transferrin concentrations and the presence of NTBI in CF and AF suggest that transferrin-independent iron transfer is important in early gestation.

Show MeSH