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Simultaneous quantification of human herpesvirus 8 DNA by real time PCR in different tissues of HIV infected cuban patients with Kaposi's sarcoma.

Kourí V, Martínez PA, Blanco O, Capó V, Rodríguez ME, Dovigny Mdel C, Cardellá L, Gala A, Jiménez NA, Correa C, Alemán Y, Pérez L, Alvarez A, Hengge U - Herpesviridae (2010)

Bottom Line: In Cuba, previous reports have shown an increase of epidemic KS, reaching a total of 120 cases by the end of 2007, despite the use of HAART.Men who had sex with men (MSM) were more likely to have three-times higher HHV-8 genome copies in KS lesions when compared with tissues from heterosexuals individuals (OR 3; 95% CI 1.1 to 12.5).These results emphasize the systemic nature of HHV-8-infection and demonstrate the possible role of saliva in HHV-8 transmission among MSM.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Tropical Medicine "Pedro Kourí", Havana City, Cuba. vkouri@ipk.sld.cu.

ABSTRACT
In Cuba, previous reports have shown an increase of epidemic KS, reaching a total of 120 cases by the end of 2007, despite the use of HAART. To evaluate and compare the role of human herpes virus 8 (HHV-8) viral loads in different compartments of AIDS-related Kaposi's sarcoma (AIDS-KS) patients real-time polymerase chain reaction (RT-PCR) was used to determine the genome copy number of HHV-8 in plasma, saliva, tissue and peripheral blood mononuclear cells (PBMC) of 49 AIDS-KS patients. Overall, 98% of AIDS-KS patients harbored detectable HHV-8. HHV-8 could be detected in 91.6% of KS tissue lesions showing the highest viral load (median log = 3.14 copies/100 ng DNA) followed by saliva and PBMC which were positive in 78%, and 69.2%; respectively. In contrast, HHV-8 was detected in only 37% of plasma samples, which also showed lower viral loads. Men who had sex with men (MSM) were more likely to have three-times higher HHV-8 genome copies in KS lesions when compared with tissues from heterosexuals individuals (OR 3; 95% CI 1.1 to 12.5). These results emphasize the systemic nature of HHV-8-infection and demonstrate the possible role of saliva in HHV-8 transmission among MSM.

No MeSH data available.


Related in: MedlinePlus

Graphic representation of KSHV loads in different samples per each AIDS-KS patient.
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Related In: Results  -  Collection


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Figure 2: Graphic representation of KSHV loads in different samples per each AIDS-KS patient.

Mentions: There was a significant Pearson's correlation when each patient's tissue HHV-8 levels were compared with saliva and PBMC (p < 0.01); however, this correlation did not exist for plasma samples (Figure 2). We did not find association between the HHV-8 viral load in all the samples analyzed with: CD4 cell counts, HIV viral load, type of KS (cutaneous, mucocutaneous and systemic), the histological stage (macular, patch and tumoral) nor the number of years elapsed between the KS diagnosis and death (p > 0.05).


Simultaneous quantification of human herpesvirus 8 DNA by real time PCR in different tissues of HIV infected cuban patients with Kaposi's sarcoma.

Kourí V, Martínez PA, Blanco O, Capó V, Rodríguez ME, Dovigny Mdel C, Cardellá L, Gala A, Jiménez NA, Correa C, Alemán Y, Pérez L, Alvarez A, Hengge U - Herpesviridae (2010)

Graphic representation of KSHV loads in different samples per each AIDS-KS patient.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3050434&req=5

Figure 2: Graphic representation of KSHV loads in different samples per each AIDS-KS patient.
Mentions: There was a significant Pearson's correlation when each patient's tissue HHV-8 levels were compared with saliva and PBMC (p < 0.01); however, this correlation did not exist for plasma samples (Figure 2). We did not find association between the HHV-8 viral load in all the samples analyzed with: CD4 cell counts, HIV viral load, type of KS (cutaneous, mucocutaneous and systemic), the histological stage (macular, patch and tumoral) nor the number of years elapsed between the KS diagnosis and death (p > 0.05).

Bottom Line: In Cuba, previous reports have shown an increase of epidemic KS, reaching a total of 120 cases by the end of 2007, despite the use of HAART.Men who had sex with men (MSM) were more likely to have three-times higher HHV-8 genome copies in KS lesions when compared with tissues from heterosexuals individuals (OR 3; 95% CI 1.1 to 12.5).These results emphasize the systemic nature of HHV-8-infection and demonstrate the possible role of saliva in HHV-8 transmission among MSM.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Tropical Medicine "Pedro Kourí", Havana City, Cuba. vkouri@ipk.sld.cu.

ABSTRACT
In Cuba, previous reports have shown an increase of epidemic KS, reaching a total of 120 cases by the end of 2007, despite the use of HAART. To evaluate and compare the role of human herpes virus 8 (HHV-8) viral loads in different compartments of AIDS-related Kaposi's sarcoma (AIDS-KS) patients real-time polymerase chain reaction (RT-PCR) was used to determine the genome copy number of HHV-8 in plasma, saliva, tissue and peripheral blood mononuclear cells (PBMC) of 49 AIDS-KS patients. Overall, 98% of AIDS-KS patients harbored detectable HHV-8. HHV-8 could be detected in 91.6% of KS tissue lesions showing the highest viral load (median log = 3.14 copies/100 ng DNA) followed by saliva and PBMC which were positive in 78%, and 69.2%; respectively. In contrast, HHV-8 was detected in only 37% of plasma samples, which also showed lower viral loads. Men who had sex with men (MSM) were more likely to have three-times higher HHV-8 genome copies in KS lesions when compared with tissues from heterosexuals individuals (OR 3; 95% CI 1.1 to 12.5). These results emphasize the systemic nature of HHV-8-infection and demonstrate the possible role of saliva in HHV-8 transmission among MSM.

No MeSH data available.


Related in: MedlinePlus