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A large expansion of the HSFY gene family in cattle shows dispersion across Yq and testis-specific expression.

Hamilton CK, Revay T, Domander R, Favetta LA, King WA - PLoS ONE (2011)

Bottom Line: Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (∼70 copies) compared with human (2 functional copies, 4 HSFY-similar copies).Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV).Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq).

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada.

ABSTRACT
Heat shock transcription factor, Y-linked (HSFY) is a member of the heat shock transcriptional factor (HSF) family that is found in multiple copies on the Y chromosome and conserved in a number of species. Its function still remains unknown but in humans it is thought to play a role in spermatogenesis. Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (∼70 copies) compared with human (2 functional copies, 4 HSFY-similar copies). Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV). Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq). HSFY expression in cattle appears restricted to the testis and its mRNA correlates positively with mRNA markers of spermatogonial and spermatocyte cells (UCHL1 and TRPC2, respectively) which suggests that HSFY is expressed (at least in part) in early germ cells.

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HSFY expression in different tissues.HSFY was analyzed in a variety of tissues including testis (T), lung (Lu), heart (H), liver (Li), kidney (K) and ovary (O) using real time PCR and two different HSFY primer sets to amplify the gene as well as the reference gene, GAPDH. The results show that both HSFYRNA (HSFYRNAF/HSFYRNAR) and HSFY8 (HSFY8F/HSFY8R) primer sets amplify HSFY only in the testis and no other tissue. All tissues show expression of GAPDH. M) 100 bp ladder (Invitrogen Canada Inc.).
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pone-0017790-g007: HSFY expression in different tissues.HSFY was analyzed in a variety of tissues including testis (T), lung (Lu), heart (H), liver (Li), kidney (K) and ovary (O) using real time PCR and two different HSFY primer sets to amplify the gene as well as the reference gene, GAPDH. The results show that both HSFYRNA (HSFYRNAF/HSFYRNAR) and HSFY8 (HSFY8F/HSFY8R) primer sets amplify HSFY only in the testis and no other tissue. All tissues show expression of GAPDH. M) 100 bp ladder (Invitrogen Canada Inc.).

Mentions: An analysis of a variety of different tissues (heart, kidney, liver, lung, ovary and testis) showed expression only in the testis for both HSFYRNA primers (spans intron 1) and HSFY8 primers (specific to exon 1) (Figure 7). GAPDH was present in all tissues.


A large expansion of the HSFY gene family in cattle shows dispersion across Yq and testis-specific expression.

Hamilton CK, Revay T, Domander R, Favetta LA, King WA - PLoS ONE (2011)

HSFY expression in different tissues.HSFY was analyzed in a variety of tissues including testis (T), lung (Lu), heart (H), liver (Li), kidney (K) and ovary (O) using real time PCR and two different HSFY primer sets to amplify the gene as well as the reference gene, GAPDH. The results show that both HSFYRNA (HSFYRNAF/HSFYRNAR) and HSFY8 (HSFY8F/HSFY8R) primer sets amplify HSFY only in the testis and no other tissue. All tissues show expression of GAPDH. M) 100 bp ladder (Invitrogen Canada Inc.).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3049798&req=5

pone-0017790-g007: HSFY expression in different tissues.HSFY was analyzed in a variety of tissues including testis (T), lung (Lu), heart (H), liver (Li), kidney (K) and ovary (O) using real time PCR and two different HSFY primer sets to amplify the gene as well as the reference gene, GAPDH. The results show that both HSFYRNA (HSFYRNAF/HSFYRNAR) and HSFY8 (HSFY8F/HSFY8R) primer sets amplify HSFY only in the testis and no other tissue. All tissues show expression of GAPDH. M) 100 bp ladder (Invitrogen Canada Inc.).
Mentions: An analysis of a variety of different tissues (heart, kidney, liver, lung, ovary and testis) showed expression only in the testis for both HSFYRNA primers (spans intron 1) and HSFY8 primers (specific to exon 1) (Figure 7). GAPDH was present in all tissues.

Bottom Line: Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (∼70 copies) compared with human (2 functional copies, 4 HSFY-similar copies).Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV).Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq).

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada.

ABSTRACT
Heat shock transcription factor, Y-linked (HSFY) is a member of the heat shock transcriptional factor (HSF) family that is found in multiple copies on the Y chromosome and conserved in a number of species. Its function still remains unknown but in humans it is thought to play a role in spermatogenesis. Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (∼70 copies) compared with human (2 functional copies, 4 HSFY-similar copies). Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV). Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq). HSFY expression in cattle appears restricted to the testis and its mRNA correlates positively with mRNA markers of spermatogonial and spermatocyte cells (UCHL1 and TRPC2, respectively) which suggests that HSFY is expressed (at least in part) in early germ cells.

Show MeSH
Related in: MedlinePlus