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A large expansion of the HSFY gene family in cattle shows dispersion across Yq and testis-specific expression.

Hamilton CK, Revay T, Domander R, Favetta LA, King WA - PLoS ONE (2011)

Bottom Line: Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (∼70 copies) compared with human (2 functional copies, 4 HSFY-similar copies).Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV).Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq).

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada.

ABSTRACT
Heat shock transcription factor, Y-linked (HSFY) is a member of the heat shock transcriptional factor (HSF) family that is found in multiple copies on the Y chromosome and conserved in a number of species. Its function still remains unknown but in humans it is thought to play a role in spermatogenesis. Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (∼70 copies) compared with human (2 functional copies, 4 HSFY-similar copies). Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV). Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq). HSFY expression in cattle appears restricted to the testis and its mRNA correlates positively with mRNA markers of spermatogonial and spermatocyte cells (UCHL1 and TRPC2, respectively) which suggests that HSFY is expressed (at least in part) in early germ cells.

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Related in: MedlinePlus

Gel electrophoresis of hybridization probes (A) and flourescence in situ hybridization (FISH) analysis (B–G).A) The largest intense band in lane 1 shows 2072 bp fragment of the 100 bp DNA ladder (Invitrogen Canada Inc.). PCR products amplified by primer pairs HSFY-E1F/HSFY-E2R, HSFY-E1F/HSFY-E1R, HSFY-E2F/HSFY-E2R were loaded into lanes 2, 3, 4, respectively. FISH images show the hybridization of the same products in the same order, thus using the whole gene (E), exon 1 (F) or exon 2 (G) specific probes. Images in the upper row (B, C, D) show the metaphase chromosomes with only 4′,6-diamidino-2-phenylindole (DAPI) counterstaining. Bar represents 10 µm.
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pone-0017790-g006: Gel electrophoresis of hybridization probes (A) and flourescence in situ hybridization (FISH) analysis (B–G).A) The largest intense band in lane 1 shows 2072 bp fragment of the 100 bp DNA ladder (Invitrogen Canada Inc.). PCR products amplified by primer pairs HSFY-E1F/HSFY-E2R, HSFY-E1F/HSFY-E1R, HSFY-E2F/HSFY-E2R were loaded into lanes 2, 3, 4, respectively. FISH images show the hybridization of the same products in the same order, thus using the whole gene (E), exon 1 (F) or exon 2 (G) specific probes. Images in the upper row (B, C, D) show the metaphase chromosomes with only 4′,6-diamidino-2-phenylindole (DAPI) counterstaining. Bar represents 10 µm.

Mentions: All three different FISH probes generated an intense, specific, yet dispersed signal on the Y chromosome. The signals were almost chromosome painting probe-like and labeled the majority of the q-arm of the Y chromosome (Figure 6). There were no differences in the hybridization pattern between the probes.


A large expansion of the HSFY gene family in cattle shows dispersion across Yq and testis-specific expression.

Hamilton CK, Revay T, Domander R, Favetta LA, King WA - PLoS ONE (2011)

Gel electrophoresis of hybridization probes (A) and flourescence in situ hybridization (FISH) analysis (B–G).A) The largest intense band in lane 1 shows 2072 bp fragment of the 100 bp DNA ladder (Invitrogen Canada Inc.). PCR products amplified by primer pairs HSFY-E1F/HSFY-E2R, HSFY-E1F/HSFY-E1R, HSFY-E2F/HSFY-E2R were loaded into lanes 2, 3, 4, respectively. FISH images show the hybridization of the same products in the same order, thus using the whole gene (E), exon 1 (F) or exon 2 (G) specific probes. Images in the upper row (B, C, D) show the metaphase chromosomes with only 4′,6-diamidino-2-phenylindole (DAPI) counterstaining. Bar represents 10 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3049798&req=5

pone-0017790-g006: Gel electrophoresis of hybridization probes (A) and flourescence in situ hybridization (FISH) analysis (B–G).A) The largest intense band in lane 1 shows 2072 bp fragment of the 100 bp DNA ladder (Invitrogen Canada Inc.). PCR products amplified by primer pairs HSFY-E1F/HSFY-E2R, HSFY-E1F/HSFY-E1R, HSFY-E2F/HSFY-E2R were loaded into lanes 2, 3, 4, respectively. FISH images show the hybridization of the same products in the same order, thus using the whole gene (E), exon 1 (F) or exon 2 (G) specific probes. Images in the upper row (B, C, D) show the metaphase chromosomes with only 4′,6-diamidino-2-phenylindole (DAPI) counterstaining. Bar represents 10 µm.
Mentions: All three different FISH probes generated an intense, specific, yet dispersed signal on the Y chromosome. The signals were almost chromosome painting probe-like and labeled the majority of the q-arm of the Y chromosome (Figure 6). There were no differences in the hybridization pattern between the probes.

Bottom Line: Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (∼70 copies) compared with human (2 functional copies, 4 HSFY-similar copies).Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV).Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq).

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada.

ABSTRACT
Heat shock transcription factor, Y-linked (HSFY) is a member of the heat shock transcriptional factor (HSF) family that is found in multiple copies on the Y chromosome and conserved in a number of species. Its function still remains unknown but in humans it is thought to play a role in spermatogenesis. Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (∼70 copies) compared with human (2 functional copies, 4 HSFY-similar copies). Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV). Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq). HSFY expression in cattle appears restricted to the testis and its mRNA correlates positively with mRNA markers of spermatogonial and spermatocyte cells (UCHL1 and TRPC2, respectively) which suggests that HSFY is expressed (at least in part) in early germ cells.

Show MeSH
Related in: MedlinePlus