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A large expansion of the HSFY gene family in cattle shows dispersion across Yq and testis-specific expression.

Hamilton CK, Revay T, Domander R, Favetta LA, King WA - PLoS ONE (2011)

Bottom Line: Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (∼70 copies) compared with human (2 functional copies, 4 HSFY-similar copies).Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV).Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq).

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada.

ABSTRACT
Heat shock transcription factor, Y-linked (HSFY) is a member of the heat shock transcriptional factor (HSF) family that is found in multiple copies on the Y chromosome and conserved in a number of species. Its function still remains unknown but in humans it is thought to play a role in spermatogenesis. Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (∼70 copies) compared with human (2 functional copies, 4 HSFY-similar copies). Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV). Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq). HSFY expression in cattle appears restricted to the testis and its mRNA correlates positively with mRNA markers of spermatogonial and spermatocyte cells (UCHL1 and TRPC2, respectively) which suggests that HSFY is expressed (at least in part) in early germ cells.

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Related in: MedlinePlus

Comparison of the predicted amino acid sequences of HSFY in Hereford and Holstein cattle.The mRNA sequences are 99% homologous and differ by only one base pair (c.1192A>C). This missense substitution mutation (p.Tyr266Ser) results in an amino acid change from tyrosine (Y) in Hereford to serine (S) in Holstein in the predicted protein sequence (shown by the box). This mutation is not found in the conserved HSF-type DNA-binding domain which is shown by the underlined sequence.
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pone-0017790-g003: Comparison of the predicted amino acid sequences of HSFY in Hereford and Holstein cattle.The mRNA sequences are 99% homologous and differ by only one base pair (c.1192A>C). This missense substitution mutation (p.Tyr266Ser) results in an amino acid change from tyrosine (Y) in Hereford to serine (S) in Holstein in the predicted protein sequence (shown by the box). This mutation is not found in the conserved HSF-type DNA-binding domain which is shown by the underlined sequence.

Mentions: The complete HSFY gene sequence was deduced in Holstein and submitted to GenBank (accession number: JF281100). The Holstein HSFY genomic sequence was found to differ with the Hereford HSFY sequence by two nucleotides (c.56G>A, c.1192A>C). The published Hereford mRNA sequence, however, also shows the first discrepancy with the Hereford genomic sequence (c.56G>A) and therefore this one mismatch likely represents a genomic sequencing error. Our Holstein genomic sequence matches the Hereford mRNA sequence at this position. The second point mutation we found (c.1192A>C) is consistently different from both the Hereford genomic and mRNA sequences currently deposited on the NCBI database. This mutation results in a substitution from tyrosine (Hereford) to serine (Holstein) at position 266 of the predicted HSFY protein (p.Tyr266Ser). An alignment of the predicted amino acid sequences for both Hereford and Holstein cattle is shown in Figure 3.


A large expansion of the HSFY gene family in cattle shows dispersion across Yq and testis-specific expression.

Hamilton CK, Revay T, Domander R, Favetta LA, King WA - PLoS ONE (2011)

Comparison of the predicted amino acid sequences of HSFY in Hereford and Holstein cattle.The mRNA sequences are 99% homologous and differ by only one base pair (c.1192A>C). This missense substitution mutation (p.Tyr266Ser) results in an amino acid change from tyrosine (Y) in Hereford to serine (S) in Holstein in the predicted protein sequence (shown by the box). This mutation is not found in the conserved HSF-type DNA-binding domain which is shown by the underlined sequence.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3049798&req=5

pone-0017790-g003: Comparison of the predicted amino acid sequences of HSFY in Hereford and Holstein cattle.The mRNA sequences are 99% homologous and differ by only one base pair (c.1192A>C). This missense substitution mutation (p.Tyr266Ser) results in an amino acid change from tyrosine (Y) in Hereford to serine (S) in Holstein in the predicted protein sequence (shown by the box). This mutation is not found in the conserved HSF-type DNA-binding domain which is shown by the underlined sequence.
Mentions: The complete HSFY gene sequence was deduced in Holstein and submitted to GenBank (accession number: JF281100). The Holstein HSFY genomic sequence was found to differ with the Hereford HSFY sequence by two nucleotides (c.56G>A, c.1192A>C). The published Hereford mRNA sequence, however, also shows the first discrepancy with the Hereford genomic sequence (c.56G>A) and therefore this one mismatch likely represents a genomic sequencing error. Our Holstein genomic sequence matches the Hereford mRNA sequence at this position. The second point mutation we found (c.1192A>C) is consistently different from both the Hereford genomic and mRNA sequences currently deposited on the NCBI database. This mutation results in a substitution from tyrosine (Hereford) to serine (Holstein) at position 266 of the predicted HSFY protein (p.Tyr266Ser). An alignment of the predicted amino acid sequences for both Hereford and Holstein cattle is shown in Figure 3.

Bottom Line: Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (∼70 copies) compared with human (2 functional copies, 4 HSFY-similar copies).Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV).Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq).

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada.

ABSTRACT
Heat shock transcription factor, Y-linked (HSFY) is a member of the heat shock transcriptional factor (HSF) family that is found in multiple copies on the Y chromosome and conserved in a number of species. Its function still remains unknown but in humans it is thought to play a role in spermatogenesis. Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (∼70 copies) compared with human (2 functional copies, 4 HSFY-similar copies). Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV). Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq). HSFY expression in cattle appears restricted to the testis and its mRNA correlates positively with mRNA markers of spermatogonial and spermatocyte cells (UCHL1 and TRPC2, respectively) which suggests that HSFY is expressed (at least in part) in early germ cells.

Show MeSH
Related in: MedlinePlus