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Modification of cell surface properties of Pseudomonas alcaligenes S22 during hydrocarbon biodegradation.

Kaczorek E, Moszyńska S, Olszanowski A - Biodegradation (2010)

Bottom Line: Biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one.The obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using Pseudomonas alcaligenes.No positive effect on the biodegradation process was observed using synthetic surfactant Triton X-100.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965, Poznań, Poland. ewa.kaczorek@put.poznan.pl

ABSTRACT
Biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one. Very promising option is the use of saponins. The obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using Pseudomonas alcaligenes. No positive effect on the biodegradation process was observed using synthetic surfactant Triton X-100. The kind of carbon source influences the cell surface properties of microorganisms. Modification of the surface cell could be observed by control of the sedimentation profile. This analytical method is a new approach in microbiological analysis.

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Related in: MedlinePlus

Diesel oil biodegradation by Pseudomonas alcaligenes strain after 7, 14 and 21 days in systems: diesel oil, diesel oil with saponins, diesel oil with rhamnolipides and diesel oil with Triton X-100. The concentration of surfactants were 120 mg/l
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Fig3: Diesel oil biodegradation by Pseudomonas alcaligenes strain after 7, 14 and 21 days in systems: diesel oil, diesel oil with saponins, diesel oil with rhamnolipides and diesel oil with Triton X-100. The concentration of surfactants were 120 mg/l

Mentions: Results of diesel oil biodegradation showed that Pseudomonas alcaligenes S22 is a good biological agent. The experiment was carried out for 21 days. Biodegradation was measured after every 7 days. After 21 days, 70% diesel oil biodegradation was noticed. The largest differences were observed between 7 and 14 days, when biodegradation increased about 100% (Fig. 3). The addition of natural surfactants to the diesel oil system caused an increase in the biodegradation. Saponins turned out to be the most effective. The biodegradation of diesel oil was 52% after 7 days. In the following days biodegradation increased and finally 92% of degradation was observed. The adhesion to hydrocarbon in these systems increased insignificantly during the process (Fig. 4). In the case of rhamnolipides the biodegradation of diesel oil was 80% after 21 days. For rhamnolipides, as well as for Triton X-100, changes in adhesion to hydrocarbons after 14 and 21 days were observed. After 21 days of the biodegradation process, the cell surface of tested strain had lower adhesion properties than after 7 days. According to Zhang and Miller (1994), a mutual attraction between biosurfactant and microbial cells can lead to an increase in cell hydrophobicity, and therefore cells have a better contact with the hydrophobic substrate finally achieving a higher biodegradation.Fig. 3


Modification of cell surface properties of Pseudomonas alcaligenes S22 during hydrocarbon biodegradation.

Kaczorek E, Moszyńska S, Olszanowski A - Biodegradation (2010)

Diesel oil biodegradation by Pseudomonas alcaligenes strain after 7, 14 and 21 days in systems: diesel oil, diesel oil with saponins, diesel oil with rhamnolipides and diesel oil with Triton X-100. The concentration of surfactants were 120 mg/l
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3046353&req=5

Fig3: Diesel oil biodegradation by Pseudomonas alcaligenes strain after 7, 14 and 21 days in systems: diesel oil, diesel oil with saponins, diesel oil with rhamnolipides and diesel oil with Triton X-100. The concentration of surfactants were 120 mg/l
Mentions: Results of diesel oil biodegradation showed that Pseudomonas alcaligenes S22 is a good biological agent. The experiment was carried out for 21 days. Biodegradation was measured after every 7 days. After 21 days, 70% diesel oil biodegradation was noticed. The largest differences were observed between 7 and 14 days, when biodegradation increased about 100% (Fig. 3). The addition of natural surfactants to the diesel oil system caused an increase in the biodegradation. Saponins turned out to be the most effective. The biodegradation of diesel oil was 52% after 7 days. In the following days biodegradation increased and finally 92% of degradation was observed. The adhesion to hydrocarbon in these systems increased insignificantly during the process (Fig. 4). In the case of rhamnolipides the biodegradation of diesel oil was 80% after 21 days. For rhamnolipides, as well as for Triton X-100, changes in adhesion to hydrocarbons after 14 and 21 days were observed. After 21 days of the biodegradation process, the cell surface of tested strain had lower adhesion properties than after 7 days. According to Zhang and Miller (1994), a mutual attraction between biosurfactant and microbial cells can lead to an increase in cell hydrophobicity, and therefore cells have a better contact with the hydrophobic substrate finally achieving a higher biodegradation.Fig. 3

Bottom Line: Biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one.The obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using Pseudomonas alcaligenes.No positive effect on the biodegradation process was observed using synthetic surfactant Triton X-100.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965, Poznań, Poland. ewa.kaczorek@put.poznan.pl

ABSTRACT
Biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one. Very promising option is the use of saponins. The obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using Pseudomonas alcaligenes. No positive effect on the biodegradation process was observed using synthetic surfactant Triton X-100. The kind of carbon source influences the cell surface properties of microorganisms. Modification of the surface cell could be observed by control of the sedimentation profile. This analytical method is a new approach in microbiological analysis.

Show MeSH
Related in: MedlinePlus