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Modification of cell surface properties of Pseudomonas alcaligenes S22 during hydrocarbon biodegradation.

Kaczorek E, Moszyńska S, Olszanowski A - Biodegradation (2010)

Bottom Line: Biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one.The obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using Pseudomonas alcaligenes.No positive effect on the biodegradation process was observed using synthetic surfactant Triton X-100.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965, Poznań, Poland. ewa.kaczorek@put.poznan.pl

ABSTRACT
Biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one. Very promising option is the use of saponins. The obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using Pseudomonas alcaligenes. No positive effect on the biodegradation process was observed using synthetic surfactant Triton X-100. The kind of carbon source influences the cell surface properties of microorganisms. Modification of the surface cell could be observed by control of the sedimentation profile. This analytical method is a new approach in microbiological analysis.

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Related in: MedlinePlus

Mass of sediment biomass of Pseudomonas alcaligenes strain versus time. Tested strain after inoculation with a different carbon source (a) and in the system with surfactants in concentration 120 mg/l (b)
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Fig2: Mass of sediment biomass of Pseudomonas alcaligenes strain versus time. Tested strain after inoculation with a different carbon source (a) and in the system with surfactants in concentration 120 mg/l (b)

Mentions: The sedimentation profile of bacterial strains depends on the kind of carbon source present in the system (Fig. 2). When cell surface of bacteria became more hydrophobic probably could be supposed, that a tendency for agglomeration of cells is observed and a rapid sedimentation is recorded. Bigger particles rapidly settle in the system and the values of the sedimentation mass are bigger than in the case of more hydrophilic bacteria cells. A higher gradient of sedimentation profile, almost for all tested systems, results in bigger values of the sedimentation mass.Fig. 2


Modification of cell surface properties of Pseudomonas alcaligenes S22 during hydrocarbon biodegradation.

Kaczorek E, Moszyńska S, Olszanowski A - Biodegradation (2010)

Mass of sediment biomass of Pseudomonas alcaligenes strain versus time. Tested strain after inoculation with a different carbon source (a) and in the system with surfactants in concentration 120 mg/l (b)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3046353&req=5

Fig2: Mass of sediment biomass of Pseudomonas alcaligenes strain versus time. Tested strain after inoculation with a different carbon source (a) and in the system with surfactants in concentration 120 mg/l (b)
Mentions: The sedimentation profile of bacterial strains depends on the kind of carbon source present in the system (Fig. 2). When cell surface of bacteria became more hydrophobic probably could be supposed, that a tendency for agglomeration of cells is observed and a rapid sedimentation is recorded. Bigger particles rapidly settle in the system and the values of the sedimentation mass are bigger than in the case of more hydrophilic bacteria cells. A higher gradient of sedimentation profile, almost for all tested systems, results in bigger values of the sedimentation mass.Fig. 2

Bottom Line: Biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one.The obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using Pseudomonas alcaligenes.No positive effect on the biodegradation process was observed using synthetic surfactant Triton X-100.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965, Poznań, Poland. ewa.kaczorek@put.poznan.pl

ABSTRACT
Biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one. Very promising option is the use of saponins. The obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using Pseudomonas alcaligenes. No positive effect on the biodegradation process was observed using synthetic surfactant Triton X-100. The kind of carbon source influences the cell surface properties of microorganisms. Modification of the surface cell could be observed by control of the sedimentation profile. This analytical method is a new approach in microbiological analysis.

Show MeSH
Related in: MedlinePlus