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Modification of cell surface properties of Pseudomonas alcaligenes S22 during hydrocarbon biodegradation.

Kaczorek E, Moszyńska S, Olszanowski A - Biodegradation (2010)

Bottom Line: Biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one.The obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using Pseudomonas alcaligenes.No positive effect on the biodegradation process was observed using synthetic surfactant Triton X-100.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965, Poznań, Poland. ewa.kaczorek@put.poznan.pl

ABSTRACT
Biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one. Very promising option is the use of saponins. The obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using Pseudomonas alcaligenes. No positive effect on the biodegradation process was observed using synthetic surfactant Triton X-100. The kind of carbon source influences the cell surface properties of microorganisms. Modification of the surface cell could be observed by control of the sedimentation profile. This analytical method is a new approach in microbiological analysis.

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Related in: MedlinePlus

Bacterial adhesion to hydrocarbon of surface cells of Pseudomonas alcaligenes strain after biodegradation process in the system with surfactants (a) and hydrocarbon-surfactants (b)
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Fig1: Bacterial adhesion to hydrocarbon of surface cells of Pseudomonas alcaligenes strain after biodegradation process in the system with surfactants (a) and hydrocarbon-surfactants (b)

Mentions: Only 0.02% of crude oil is soluble in water, therefore, the emulsification of oil is necessary for improving the bioavaibility of insoluble hydrocarbon to the microorganism (Gerson 1993). An addition of surfactants to the system changed the cell surface properties of the tested strain. The modification of the cell surface depends on the kind and amount of surfactant (Fig. 1). Only for Triton X-100 was the higher adhesion to hydrocarbon observed than for the parent strain. For saponins up to 120 mg/l, a decrease of adhesion to hydrocarbon was registered and then, an increase to the value of 24%. Rhamnolipides did not cause any significant changes in the system (Fig. 1a). There is a difference between bacterial cell adhesion to hydrocarbon measured in systems where hydrocarbons are supplemented with surfactants and those systems with surfactants alone. This is especially observed in the case of natural surfactants: rhamnolipides and saponins (Fig 1b). Synthetic Triton X-100 caused a decrease in adhesion to hydrocarbon in the system with hydrocarbon. Surfactants increase the interfacial area between the aqueous-insoluble hydrocarbons. Beal and Betts (2000) suggested that surfactants only act to enhance the uptake of hydrophobic hydrocarbons in Ps. aeruginosa and are not required for the actual process to take place.Fig. 1


Modification of cell surface properties of Pseudomonas alcaligenes S22 during hydrocarbon biodegradation.

Kaczorek E, Moszyńska S, Olszanowski A - Biodegradation (2010)

Bacterial adhesion to hydrocarbon of surface cells of Pseudomonas alcaligenes strain after biodegradation process in the system with surfactants (a) and hydrocarbon-surfactants (b)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3046353&req=5

Fig1: Bacterial adhesion to hydrocarbon of surface cells of Pseudomonas alcaligenes strain after biodegradation process in the system with surfactants (a) and hydrocarbon-surfactants (b)
Mentions: Only 0.02% of crude oil is soluble in water, therefore, the emulsification of oil is necessary for improving the bioavaibility of insoluble hydrocarbon to the microorganism (Gerson 1993). An addition of surfactants to the system changed the cell surface properties of the tested strain. The modification of the cell surface depends on the kind and amount of surfactant (Fig. 1). Only for Triton X-100 was the higher adhesion to hydrocarbon observed than for the parent strain. For saponins up to 120 mg/l, a decrease of adhesion to hydrocarbon was registered and then, an increase to the value of 24%. Rhamnolipides did not cause any significant changes in the system (Fig. 1a). There is a difference between bacterial cell adhesion to hydrocarbon measured in systems where hydrocarbons are supplemented with surfactants and those systems with surfactants alone. This is especially observed in the case of natural surfactants: rhamnolipides and saponins (Fig 1b). Synthetic Triton X-100 caused a decrease in adhesion to hydrocarbon in the system with hydrocarbon. Surfactants increase the interfacial area between the aqueous-insoluble hydrocarbons. Beal and Betts (2000) suggested that surfactants only act to enhance the uptake of hydrophobic hydrocarbons in Ps. aeruginosa and are not required for the actual process to take place.Fig. 1

Bottom Line: Biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one.The obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using Pseudomonas alcaligenes.No positive effect on the biodegradation process was observed using synthetic surfactant Triton X-100.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965, Poznań, Poland. ewa.kaczorek@put.poznan.pl

ABSTRACT
Biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one. Very promising option is the use of saponins. The obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using Pseudomonas alcaligenes. No positive effect on the biodegradation process was observed using synthetic surfactant Triton X-100. The kind of carbon source influences the cell surface properties of microorganisms. Modification of the surface cell could be observed by control of the sedimentation profile. This analytical method is a new approach in microbiological analysis.

Show MeSH
Related in: MedlinePlus