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The Populus Class III HD ZIP transcription factor POPCORONA affects cell differentiation during secondary growth of woody stems.

Du J, Miura E, Robischon M, Martinez C, Groover A - PLoS ONE (2011)

Bottom Line: Synthetic miRNA knock down of POPCORONA results in abnormal lignification in cells of the pith, while overexpression of a miRNA-resistant POPCORONA results in delayed lignification of xylem and phloem fibers during secondary growth.POPCORONA misexpression also results in coordinated changes in expression of genes within a previously described transcriptional network regulating cell differentiation and cell wall biosynthesis, and hormone-related genes associated with fiber differentiation.POPCORONA illustrates another function of Class III HD ZIPs: regulating cell differentiation during secondary growth.

View Article: PubMed Central - PubMed

Affiliation: Institute of Forest Genetics, Pacific Southwest Research Station, U.S. Forest Service, Davis, California, United States of America.

ABSTRACT
The developmental mechanisms regulating cell differentiation and patterning during the secondary growth of woody tissues are poorly understood. Class III HD ZIP transcription factors are evolutionarily ancient and play fundamental roles in various aspects of plant development. Here we investigate the role of a Class III HD ZIP transcription factor, POPCORONA, during secondary growth of woody stems. Transgenic Populus (poplar) trees expressing either a miRNA-resistant POPCORONA or a synthetic miRNA targeting POPCORONA were used to infer function of POPCORONA during secondary growth. Whole plant, histological, and gene expression changes were compared for transgenic and wild-type control plants. Synthetic miRNA knock down of POPCORONA results in abnormal lignification in cells of the pith, while overexpression of a miRNA-resistant POPCORONA results in delayed lignification of xylem and phloem fibers during secondary growth. POPCORONA misexpression also results in coordinated changes in expression of genes within a previously described transcriptional network regulating cell differentiation and cell wall biosynthesis, and hormone-related genes associated with fiber differentiation. POPCORONA illustrates another function of Class III HD ZIPs: regulating cell differentiation during secondary growth.

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Expression of PCN (Fig. 2a) and PCN paralog Pt-ATHB.11 (Fig. 2b) in organs, as assayed by Quantitative Real Time PCR.Relative expression of PCN and paralog Pt-ATHB.11 in apices, leaves, roots, and stem was determined using Quantitative Real Time PCR (QRT-PCR) of two month old tissue culture grown Populus tremula x alba. PCN and paralog Pt-ATHB.11 are expressed in all tissues assayed, and are highly expressed in shoot apexes and stem tissue with active cambium. Stem tissue samples were confirmed to have a vascular cambium by phloroglucinol staining of secondary xylem. Relative expression (Mean ± SE) was calculated from triplicate QRT-PCR reactions of independent RNA samples prepared from different trees.
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pone-0017458-g002: Expression of PCN (Fig. 2a) and PCN paralog Pt-ATHB.11 (Fig. 2b) in organs, as assayed by Quantitative Real Time PCR.Relative expression of PCN and paralog Pt-ATHB.11 in apices, leaves, roots, and stem was determined using Quantitative Real Time PCR (QRT-PCR) of two month old tissue culture grown Populus tremula x alba. PCN and paralog Pt-ATHB.11 are expressed in all tissues assayed, and are highly expressed in shoot apexes and stem tissue with active cambium. Stem tissue samples were confirmed to have a vascular cambium by phloroglucinol staining of secondary xylem. Relative expression (Mean ± SE) was calculated from triplicate QRT-PCR reactions of independent RNA samples prepared from different trees.

Mentions: PCN is expressed in shoot apices, leaves, stems, and roots of young Populus plants. Transcript levels were determined for organs of tissue culture-grown Populus alba x tremula using quantitative real time PCR (Materials and Methods). Primers amplifying PCN transcripts revealed highest expression in stems and apices, with lower but significant expression in leaves and roots (Fig. 2a). Primers amplifying a PCN paralog, Pt-ATHB.11, showed similar expression patterns for this gene, although expression was slightly higher in apices relative to stem tissues (Fig. 2b).


The Populus Class III HD ZIP transcription factor POPCORONA affects cell differentiation during secondary growth of woody stems.

Du J, Miura E, Robischon M, Martinez C, Groover A - PLoS ONE (2011)

Expression of PCN (Fig. 2a) and PCN paralog Pt-ATHB.11 (Fig. 2b) in organs, as assayed by Quantitative Real Time PCR.Relative expression of PCN and paralog Pt-ATHB.11 in apices, leaves, roots, and stem was determined using Quantitative Real Time PCR (QRT-PCR) of two month old tissue culture grown Populus tremula x alba. PCN and paralog Pt-ATHB.11 are expressed in all tissues assayed, and are highly expressed in shoot apexes and stem tissue with active cambium. Stem tissue samples were confirmed to have a vascular cambium by phloroglucinol staining of secondary xylem. Relative expression (Mean ± SE) was calculated from triplicate QRT-PCR reactions of independent RNA samples prepared from different trees.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3046250&req=5

pone-0017458-g002: Expression of PCN (Fig. 2a) and PCN paralog Pt-ATHB.11 (Fig. 2b) in organs, as assayed by Quantitative Real Time PCR.Relative expression of PCN and paralog Pt-ATHB.11 in apices, leaves, roots, and stem was determined using Quantitative Real Time PCR (QRT-PCR) of two month old tissue culture grown Populus tremula x alba. PCN and paralog Pt-ATHB.11 are expressed in all tissues assayed, and are highly expressed in shoot apexes and stem tissue with active cambium. Stem tissue samples were confirmed to have a vascular cambium by phloroglucinol staining of secondary xylem. Relative expression (Mean ± SE) was calculated from triplicate QRT-PCR reactions of independent RNA samples prepared from different trees.
Mentions: PCN is expressed in shoot apices, leaves, stems, and roots of young Populus plants. Transcript levels were determined for organs of tissue culture-grown Populus alba x tremula using quantitative real time PCR (Materials and Methods). Primers amplifying PCN transcripts revealed highest expression in stems and apices, with lower but significant expression in leaves and roots (Fig. 2a). Primers amplifying a PCN paralog, Pt-ATHB.11, showed similar expression patterns for this gene, although expression was slightly higher in apices relative to stem tissues (Fig. 2b).

Bottom Line: Synthetic miRNA knock down of POPCORONA results in abnormal lignification in cells of the pith, while overexpression of a miRNA-resistant POPCORONA results in delayed lignification of xylem and phloem fibers during secondary growth.POPCORONA misexpression also results in coordinated changes in expression of genes within a previously described transcriptional network regulating cell differentiation and cell wall biosynthesis, and hormone-related genes associated with fiber differentiation.POPCORONA illustrates another function of Class III HD ZIPs: regulating cell differentiation during secondary growth.

View Article: PubMed Central - PubMed

Affiliation: Institute of Forest Genetics, Pacific Southwest Research Station, U.S. Forest Service, Davis, California, United States of America.

ABSTRACT
The developmental mechanisms regulating cell differentiation and patterning during the secondary growth of woody tissues are poorly understood. Class III HD ZIP transcription factors are evolutionarily ancient and play fundamental roles in various aspects of plant development. Here we investigate the role of a Class III HD ZIP transcription factor, POPCORONA, during secondary growth of woody stems. Transgenic Populus (poplar) trees expressing either a miRNA-resistant POPCORONA or a synthetic miRNA targeting POPCORONA were used to infer function of POPCORONA during secondary growth. Whole plant, histological, and gene expression changes were compared for transgenic and wild-type control plants. Synthetic miRNA knock down of POPCORONA results in abnormal lignification in cells of the pith, while overexpression of a miRNA-resistant POPCORONA results in delayed lignification of xylem and phloem fibers during secondary growth. POPCORONA misexpression also results in coordinated changes in expression of genes within a previously described transcriptional network regulating cell differentiation and cell wall biosynthesis, and hormone-related genes associated with fiber differentiation. POPCORONA illustrates another function of Class III HD ZIPs: regulating cell differentiation during secondary growth.

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