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The RHO-1 RhoGTPase modulates fertility and multiple behaviors in adult C. elegans.

McMullan R, Nurrish SJ - PLoS ONE (2011)

Bottom Line: Changes in RHO-1 signaling in cholinergic neurons affected locomotion, pharyngeal pumping and fecundity.Changes in RHO-1 signaling outside the cholinergic neurons resulted in defective defecation, ovulation, and changes in C. elegans body morphology.Finally both increased and decreased RHO-1 signaling in adults resulted in death within hours.

View Article: PubMed Central - PubMed

Affiliation: MRC Cell Biology Unit, MRC Laboratory for Molecular Cell Biology and Department of Neuroscience, Physiology and Pharmacology, University College, London, United Kingdom.

ABSTRACT
The Rho family of small GTPases are essential during early embryonic development making it difficult to study their functions in adult animals. Using inducible transgenes expressing either a constitutively active version of the single C. elegans Rho ortholog, RHO-1, or an inhibitor of endogenous Rho (C3 transferase), we demonstrate multiple defects caused by altering Rho signaling in adult C. elegans. Changes in RHO-1 signaling in cholinergic neurons affected locomotion, pharyngeal pumping and fecundity. Changes in RHO-1 signaling outside the cholinergic neurons resulted in defective defecation, ovulation, and changes in C. elegans body morphology. Finally both increased and decreased RHO-1 signaling in adults resulted in death within hours. The multiple post-developmental roles for Rho in C. elegans demonstrate that RhoA signaling pathways continue to be used post-developmentally and the resulting phenotypes provide an opportunity to further study post-developmental Rho signaling pathways using genetic screens.

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Related in: MedlinePlus

Overexpression of hsRHO-1(G14V) results in anatomical changes.After heat shock abnormalities were observed in the vulva of animals expressing hsRHO-1(G14V) (nzIs1). Wild type controls (A and B), animals expressing hsC3 transferase (nzEx4) (E and F) and animals containing the hsRHO-1(G14V) (nzIs1) transgene in the absence of heat shock (C) had normal unswollen vulva however following heat shock of hsRHO-1(G14V) (nzIs1) animals a phenotype reminiscent of pvl was observed (D).
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pone-0017265-g006: Overexpression of hsRHO-1(G14V) results in anatomical changes.After heat shock abnormalities were observed in the vulva of animals expressing hsRHO-1(G14V) (nzIs1). Wild type controls (A and B), animals expressing hsC3 transferase (nzEx4) (E and F) and animals containing the hsRHO-1(G14V) (nzIs1) transgene in the absence of heat shock (C) had normal unswollen vulva however following heat shock of hsRHO-1(G14V) (nzIs1) animals a phenotype reminiscent of pvl was observed (D).

Mentions: During the course of our studies we observed changes in the anatomy of animals expressing hsRHO-1(G14V). Animals induced to express hsRHO-1(G14V) by heat shock at 33°C (Figure 6D) and then allowed to recover developed swellings around the vulval (Figure 6C and D) and anal (data not shown) regions. These swellings became visible 6 hours after heat shock, had a penetrance of 90% and 93% respectively 12 hours after heat shock and resembled the pvl and dar phenotypes previously reported [25], [46]. We further describe a role for Rho signaling in regulating the dar phenotype following infection elsewhere (RM and SN paper in preparation). We did not observe these phenotypes in wild type animals or animals expressing hsC3 transferase subjected to identical heat shock regimes (Figure 6A, B, E and F) and therefore these phenotypes appear to be a consequence of increased RHO-1 signaling.


The RHO-1 RhoGTPase modulates fertility and multiple behaviors in adult C. elegans.

McMullan R, Nurrish SJ - PLoS ONE (2011)

Overexpression of hsRHO-1(G14V) results in anatomical changes.After heat shock abnormalities were observed in the vulva of animals expressing hsRHO-1(G14V) (nzIs1). Wild type controls (A and B), animals expressing hsC3 transferase (nzEx4) (E and F) and animals containing the hsRHO-1(G14V) (nzIs1) transgene in the absence of heat shock (C) had normal unswollen vulva however following heat shock of hsRHO-1(G14V) (nzIs1) animals a phenotype reminiscent of pvl was observed (D).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3046162&req=5

pone-0017265-g006: Overexpression of hsRHO-1(G14V) results in anatomical changes.After heat shock abnormalities were observed in the vulva of animals expressing hsRHO-1(G14V) (nzIs1). Wild type controls (A and B), animals expressing hsC3 transferase (nzEx4) (E and F) and animals containing the hsRHO-1(G14V) (nzIs1) transgene in the absence of heat shock (C) had normal unswollen vulva however following heat shock of hsRHO-1(G14V) (nzIs1) animals a phenotype reminiscent of pvl was observed (D).
Mentions: During the course of our studies we observed changes in the anatomy of animals expressing hsRHO-1(G14V). Animals induced to express hsRHO-1(G14V) by heat shock at 33°C (Figure 6D) and then allowed to recover developed swellings around the vulval (Figure 6C and D) and anal (data not shown) regions. These swellings became visible 6 hours after heat shock, had a penetrance of 90% and 93% respectively 12 hours after heat shock and resembled the pvl and dar phenotypes previously reported [25], [46]. We further describe a role for Rho signaling in regulating the dar phenotype following infection elsewhere (RM and SN paper in preparation). We did not observe these phenotypes in wild type animals or animals expressing hsC3 transferase subjected to identical heat shock regimes (Figure 6A, B, E and F) and therefore these phenotypes appear to be a consequence of increased RHO-1 signaling.

Bottom Line: Changes in RHO-1 signaling in cholinergic neurons affected locomotion, pharyngeal pumping and fecundity.Changes in RHO-1 signaling outside the cholinergic neurons resulted in defective defecation, ovulation, and changes in C. elegans body morphology.Finally both increased and decreased RHO-1 signaling in adults resulted in death within hours.

View Article: PubMed Central - PubMed

Affiliation: MRC Cell Biology Unit, MRC Laboratory for Molecular Cell Biology and Department of Neuroscience, Physiology and Pharmacology, University College, London, United Kingdom.

ABSTRACT
The Rho family of small GTPases are essential during early embryonic development making it difficult to study their functions in adult animals. Using inducible transgenes expressing either a constitutively active version of the single C. elegans Rho ortholog, RHO-1, or an inhibitor of endogenous Rho (C3 transferase), we demonstrate multiple defects caused by altering Rho signaling in adult C. elegans. Changes in RHO-1 signaling in cholinergic neurons affected locomotion, pharyngeal pumping and fecundity. Changes in RHO-1 signaling outside the cholinergic neurons resulted in defective defecation, ovulation, and changes in C. elegans body morphology. Finally both increased and decreased RHO-1 signaling in adults resulted in death within hours. The multiple post-developmental roles for Rho in C. elegans demonstrate that RhoA signaling pathways continue to be used post-developmentally and the resulting phenotypes provide an opportunity to further study post-developmental Rho signaling pathways using genetic screens.

Show MeSH
Related in: MedlinePlus