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Lactobacillus rhamnosus HN001 attenuates allergy development in a pig model.

Thomas DJ, Husmann RJ, Villamar M, Winship TR, Buck RH, Zuckermann FA - PLoS ONE (2011)

Bottom Line: This study was conducted to determine the effects of the probiotic HN001 on the development of allergic lung disease in a pig model.These observations suggest that differences in clinical responses to the allergen challenges may be related to probiotic-induced modulation of Th1 (IFN-γ) and regulatory (IL-10) cytokine expression.A similar correlation between certain allergic responses and increased IFN-γ expression has been reported in human clinical studies of allergy; this pig model of allergy may be indicative of potential probiotic modulation of allergic lung disease in humans.

View Article: PubMed Central - PubMed

Affiliation: Abbott Nutrition, Columbus, Ohio, United States of America. debra.thomas@abbott.com

ABSTRACT

Background: Probiotics have been studied as immunomodulatory agents of allergy. Several human probiotic trials tracking the development of eczema and other forms of allergy have yielded inconsistent results. A recent infant study demonstrated that pre and postnatal Lactobacillus rhamnosus HN001 (HN001) supplementation decreased the prevalence of eczema and IgE associated eczema. However, the influence of HN001 on the incidence of wheeze, asthma, and/or other allergic manifestations has yet to be reported.

Objective: This study was conducted to determine the effects of the probiotic HN001 on the development of allergic lung disease in a pig model.

Methods: Allergy was induced by a series of subcutaneous and intratracheal sensitizations with Ascaris suum allergen (ASA) during a six week time frame in post-weanling pigs supplemented daily with HN001, or without supplementation. One week following final sensitization intradermal skin tests and respiratory challenges were conducted.

Results: In response to intradermal and respiratory challenges, ASA-sensitized pigs fed HN001 had less severe skin flare reactions, smaller increases in pleural pressure, and trends towards lower changes in arterial oxygen and carbon dioxide partial pressure levels compared to control pigs. The frequency of ASA-specific IFN-γ-secreting peripheral blood mononuclear cells, as well as the amount of IL-10 produced by ASA-specific cells, was of greater magnitude in probiotic-fed pigs compared to control animals. These observations suggest that differences in clinical responses to the allergen challenges may be related to probiotic-induced modulation of Th1 (IFN-γ) and regulatory (IL-10) cytokine expression.

Conclusions: Probiotic supplementation decreased the severity of allergic skin and lung responses in allergen-sensitized pigs with a corresponding increase in IFN-γ expression. A similar correlation between certain allergic responses and increased IFN-γ expression has been reported in human clinical studies of allergy; this pig model of allergy may be indicative of potential probiotic modulation of allergic lung disease in humans.

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Changes in pO2 and pCO2 levels post-respiratory challenge with ASA.Saphenous arterial blood samples were collected from ASA-sensitized pigs at the indicated times prior to and after intratracheal ASA challenge on study day 70. Blood gas levels (pO2 and pCO2) from these samples were determined and the average values are presented as the mean ± SEM percent change relative to the baseline (0 minutes) value for the probiotic-fed pigs (n = 6) and control pigs (n = 5). Average percent changes for pO2 levels in probiotic-fed pigs (filled circles) and in control animals (filled triangles) and for pCO2 levels in probiotic-fed pigs (open circles) and in control animals (open triangles) are shown. Differences between the relative percent change in pO2 and pCO2 levels for the probiotic-fed and control groups is indicated by ‡ (0.051≤p ≤0.1) (Student's t test, unpaired).
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pone-0016577-g006: Changes in pO2 and pCO2 levels post-respiratory challenge with ASA.Saphenous arterial blood samples were collected from ASA-sensitized pigs at the indicated times prior to and after intratracheal ASA challenge on study day 70. Blood gas levels (pO2 and pCO2) from these samples were determined and the average values are presented as the mean ± SEM percent change relative to the baseline (0 minutes) value for the probiotic-fed pigs (n = 6) and control pigs (n = 5). Average percent changes for pO2 levels in probiotic-fed pigs (filled circles) and in control animals (filled triangles) and for pCO2 levels in probiotic-fed pigs (open circles) and in control animals (open triangles) are shown. Differences between the relative percent change in pO2 and pCO2 levels for the probiotic-fed and control groups is indicated by ‡ (0.051≤p ≤0.1) (Student's t test, unpaired).

Mentions: When comparing lung function assessments between groups, the probiotic-fed pigs exhibited a lower average percent change in RL from baseline at all time-points as compared to the control group, but due to the variability among animals, especially in the control group, the group means were not statistically significant (Figure 4). No differences in lung Cdyn were noted between groups (Figure 4). Five minutes post-respiratory challenge the percent change in Pp (p = 0.032, Figure 5), pO2 levels (p = 0.075, Figure 5) and pCO2 levels (p = 0.058, Figure 6) was less or tended to be less in the probiotic-fed group. Ten minutes post-respiratory challenge, the percent change in pO2 levels (p = 0.076, Figure 6) also tended to be less in the probiotic group. However, there were no differences between groups in these lung function assessments by repeated measures analysis.


Lactobacillus rhamnosus HN001 attenuates allergy development in a pig model.

Thomas DJ, Husmann RJ, Villamar M, Winship TR, Buck RH, Zuckermann FA - PLoS ONE (2011)

Changes in pO2 and pCO2 levels post-respiratory challenge with ASA.Saphenous arterial blood samples were collected from ASA-sensitized pigs at the indicated times prior to and after intratracheal ASA challenge on study day 70. Blood gas levels (pO2 and pCO2) from these samples were determined and the average values are presented as the mean ± SEM percent change relative to the baseline (0 minutes) value for the probiotic-fed pigs (n = 6) and control pigs (n = 5). Average percent changes for pO2 levels in probiotic-fed pigs (filled circles) and in control animals (filled triangles) and for pCO2 levels in probiotic-fed pigs (open circles) and in control animals (open triangles) are shown. Differences between the relative percent change in pO2 and pCO2 levels for the probiotic-fed and control groups is indicated by ‡ (0.051≤p ≤0.1) (Student's t test, unpaired).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3046142&req=5

pone-0016577-g006: Changes in pO2 and pCO2 levels post-respiratory challenge with ASA.Saphenous arterial blood samples were collected from ASA-sensitized pigs at the indicated times prior to and after intratracheal ASA challenge on study day 70. Blood gas levels (pO2 and pCO2) from these samples were determined and the average values are presented as the mean ± SEM percent change relative to the baseline (0 minutes) value for the probiotic-fed pigs (n = 6) and control pigs (n = 5). Average percent changes for pO2 levels in probiotic-fed pigs (filled circles) and in control animals (filled triangles) and for pCO2 levels in probiotic-fed pigs (open circles) and in control animals (open triangles) are shown. Differences between the relative percent change in pO2 and pCO2 levels for the probiotic-fed and control groups is indicated by ‡ (0.051≤p ≤0.1) (Student's t test, unpaired).
Mentions: When comparing lung function assessments between groups, the probiotic-fed pigs exhibited a lower average percent change in RL from baseline at all time-points as compared to the control group, but due to the variability among animals, especially in the control group, the group means were not statistically significant (Figure 4). No differences in lung Cdyn were noted between groups (Figure 4). Five minutes post-respiratory challenge the percent change in Pp (p = 0.032, Figure 5), pO2 levels (p = 0.075, Figure 5) and pCO2 levels (p = 0.058, Figure 6) was less or tended to be less in the probiotic-fed group. Ten minutes post-respiratory challenge, the percent change in pO2 levels (p = 0.076, Figure 6) also tended to be less in the probiotic group. However, there were no differences between groups in these lung function assessments by repeated measures analysis.

Bottom Line: This study was conducted to determine the effects of the probiotic HN001 on the development of allergic lung disease in a pig model.These observations suggest that differences in clinical responses to the allergen challenges may be related to probiotic-induced modulation of Th1 (IFN-γ) and regulatory (IL-10) cytokine expression.A similar correlation between certain allergic responses and increased IFN-γ expression has been reported in human clinical studies of allergy; this pig model of allergy may be indicative of potential probiotic modulation of allergic lung disease in humans.

View Article: PubMed Central - PubMed

Affiliation: Abbott Nutrition, Columbus, Ohio, United States of America. debra.thomas@abbott.com

ABSTRACT

Background: Probiotics have been studied as immunomodulatory agents of allergy. Several human probiotic trials tracking the development of eczema and other forms of allergy have yielded inconsistent results. A recent infant study demonstrated that pre and postnatal Lactobacillus rhamnosus HN001 (HN001) supplementation decreased the prevalence of eczema and IgE associated eczema. However, the influence of HN001 on the incidence of wheeze, asthma, and/or other allergic manifestations has yet to be reported.

Objective: This study was conducted to determine the effects of the probiotic HN001 on the development of allergic lung disease in a pig model.

Methods: Allergy was induced by a series of subcutaneous and intratracheal sensitizations with Ascaris suum allergen (ASA) during a six week time frame in post-weanling pigs supplemented daily with HN001, or without supplementation. One week following final sensitization intradermal skin tests and respiratory challenges were conducted.

Results: In response to intradermal and respiratory challenges, ASA-sensitized pigs fed HN001 had less severe skin flare reactions, smaller increases in pleural pressure, and trends towards lower changes in arterial oxygen and carbon dioxide partial pressure levels compared to control pigs. The frequency of ASA-specific IFN-γ-secreting peripheral blood mononuclear cells, as well as the amount of IL-10 produced by ASA-specific cells, was of greater magnitude in probiotic-fed pigs compared to control animals. These observations suggest that differences in clinical responses to the allergen challenges may be related to probiotic-induced modulation of Th1 (IFN-γ) and regulatory (IL-10) cytokine expression.

Conclusions: Probiotic supplementation decreased the severity of allergic skin and lung responses in allergen-sensitized pigs with a corresponding increase in IFN-γ expression. A similar correlation between certain allergic responses and increased IFN-γ expression has been reported in human clinical studies of allergy; this pig model of allergy may be indicative of potential probiotic modulation of allergic lung disease in humans.

Show MeSH
Related in: MedlinePlus