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Robust automated tumour segmentation on histological and immunohistochemical tissue images.

Wang CW, Fennell D, Paul I, Savage K, Hamilton P - PLoS ONE (2011)

Bottom Line: The presented technique greatly improves the process of TMA construction and plays an important role in automated IHC quantification in biomarker analysis where excluding stroma areas is critical.With the finest pixel-based evaluation (instead of area-based or object-based), the experimental results show that the proposed method is able to achieve 80% accuracy and 78% accuracy in two different types of pathological virtual slides, i.e., routine histochemical H&E and IHC images, respectively.The presented technique greatly reduces labor-intensive workloads for pathologists and highly speeds up the process of TMA construction and provides a possibility for fully automated IHC quantification.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei City, Taiwan. cweiwang@mail.ntust.edu.tw

ABSTRACT
Tissue microarray (TMA) is a high throughput analysis tool to identify new diagnostic and prognostic markers in human cancers. However, standard automated method in tumour detection on both routine histochemical and immunohistochemistry (IHC) images is under developed. This paper presents a robust automated tumour cell segmentation model which can be applied to both routine histochemical tissue slides and IHC slides and deal with finer pixel-based segmentation in comparison with blob or area based segmentation by existing approaches. The presented technique greatly improves the process of TMA construction and plays an important role in automated IHC quantification in biomarker analysis where excluding stroma areas is critical. With the finest pixel-based evaluation (instead of area-based or object-based), the experimental results show that the proposed method is able to achieve 80% accuracy and 78% accuracy in two different types of pathological virtual slides, i.e., routine histochemical H&E and IHC images, respectively. The presented technique greatly reduces labor-intensive workloads for pathologists and highly speeds up the process of TMA construction and provides a possibility for fully automated IHC quantification.

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Related in: MedlinePlus

Evaluation outputs of tumour detection on routine H&E.green = TP, red = TN, yellow = false negative, and blue = false positive.
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pone-0015818-g003: Evaluation outputs of tumour detection on routine H&E.green = TP, red = TN, yellow = false negative, and blue = false positive.

Mentions: The quantitative results are shown in Table 1. Overall, the presented system achieves 80% accuracy and 79% precision in pixel based cancerous cell segmentation, and the image outputs based on the evaluation results are displayed in Fig. 3, showing that the technique is able to in identify cancerous cell on low contrast H&E lung tissue core images.


Robust automated tumour segmentation on histological and immunohistochemical tissue images.

Wang CW, Fennell D, Paul I, Savage K, Hamilton P - PLoS ONE (2011)

Evaluation outputs of tumour detection on routine H&E.green = TP, red = TN, yellow = false negative, and blue = false positive.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3046129&req=5

pone-0015818-g003: Evaluation outputs of tumour detection on routine H&E.green = TP, red = TN, yellow = false negative, and blue = false positive.
Mentions: The quantitative results are shown in Table 1. Overall, the presented system achieves 80% accuracy and 79% precision in pixel based cancerous cell segmentation, and the image outputs based on the evaluation results are displayed in Fig. 3, showing that the technique is able to in identify cancerous cell on low contrast H&E lung tissue core images.

Bottom Line: The presented technique greatly improves the process of TMA construction and plays an important role in automated IHC quantification in biomarker analysis where excluding stroma areas is critical.With the finest pixel-based evaluation (instead of area-based or object-based), the experimental results show that the proposed method is able to achieve 80% accuracy and 78% accuracy in two different types of pathological virtual slides, i.e., routine histochemical H&E and IHC images, respectively.The presented technique greatly reduces labor-intensive workloads for pathologists and highly speeds up the process of TMA construction and provides a possibility for fully automated IHC quantification.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei City, Taiwan. cweiwang@mail.ntust.edu.tw

ABSTRACT
Tissue microarray (TMA) is a high throughput analysis tool to identify new diagnostic and prognostic markers in human cancers. However, standard automated method in tumour detection on both routine histochemical and immunohistochemistry (IHC) images is under developed. This paper presents a robust automated tumour cell segmentation model which can be applied to both routine histochemical tissue slides and IHC slides and deal with finer pixel-based segmentation in comparison with blob or area based segmentation by existing approaches. The presented technique greatly improves the process of TMA construction and plays an important role in automated IHC quantification in biomarker analysis where excluding stroma areas is critical. With the finest pixel-based evaluation (instead of area-based or object-based), the experimental results show that the proposed method is able to achieve 80% accuracy and 78% accuracy in two different types of pathological virtual slides, i.e., routine histochemical H&E and IHC images, respectively. The presented technique greatly reduces labor-intensive workloads for pathologists and highly speeds up the process of TMA construction and provides a possibility for fully automated IHC quantification.

Show MeSH
Related in: MedlinePlus