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Genotypic and phenotypic modifications of Neisseria meningitidis after an accidental human passage.

Omer H, Rose G, Jolley KA, Frapy E, Zahar JR, Maiden MC, Bentley SD, Tinsley CR, Nassif X, Bille E - PLoS ONE (2011)

Bottom Line: By comparing the number of SNP in all three isolates and knowing the number of passages between Z5463 and Z5463PI, we concluded that around 25 bacterial divisions occurred in the human body.Different pilin variants were found after the in vivo passage, which expressed different properties of adhesion.Furthermore the deletion of one gene involved in LOS biosynthesis (lgtB) results in Z5463BC expressing a different LOS than the L9 immunotype of Z2491.

View Article: PubMed Central - PubMed

Affiliation: INSERM U1002, Paris, France.

ABSTRACT
A scientist in our laboratory was accidentally infected while working with Z5463, a Neisseria meningitidis serogroup A strain. She developed severe symptoms (fever, meningism, purpuric lesions) that fortunately evolved with antibiotic treatment to complete recovery. Pulse-field gel electrophoresis confirmed that the isolate obtained from the blood culture (Z5463BC) was identical to Z5463, more precisely to a fourth subculture of this strain used the week before the contamination (Z5463PI). In order to get some insights into genomic modifications that can occur in vivo, we sequenced these three isolates. All the strains contained a mutated mutS allele and therefore displayed an hypermutator phenotype, consistent with the high number of mutations (SNP, Single Nucleotide Polymorphism) detected in the three strains. By comparing the number of SNP in all three isolates and knowing the number of passages between Z5463 and Z5463PI, we concluded that around 25 bacterial divisions occurred in the human body. As expected, the in vivo passage is responsible for several modifications of phase variable genes. This genomic study has been completed by transcriptomic and phenotypic studies, showing that the blood strain used a different haemoglobin-linked iron receptor (HpuA/B) than the parental strains (HmbR). Different pilin variants were found after the in vivo passage, which expressed different properties of adhesion. Furthermore the deletion of one gene involved in LOS biosynthesis (lgtB) results in Z5463BC expressing a different LOS than the L9 immunotype of Z2491. The in vivo passage, despite the small numbers of divisions, permits the selection of numerous genomic modifications that may account for the high capacity of the strain to disseminate.

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LOS variation analysis of the strain isolated from the blood culture.A- Schematic representation of the immunotype L9 LOS of serogroup A Nm Z2491 (adapted from Zhu and coworkers [68] and Choudhury and coworkers [52]). The deletion of the lgtB gene in Z5463BC is predicted to form a truncated LOS lacking the galactose on the terminal GlcNAc. B- Decreased size of the LOS from Z5463BC due to the loss of lgtB. Tricine SDS-PAGE gel of proteinase K LOS extracts from Z5463BC and its parental strains, revealed by silver staining. 1: Z2491 (L9 immunotype), 2: Z5463, 3: Z5463PI, 4: Z5463BC.
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pone-0017145-g005: LOS variation analysis of the strain isolated from the blood culture.A- Schematic representation of the immunotype L9 LOS of serogroup A Nm Z2491 (adapted from Zhu and coworkers [68] and Choudhury and coworkers [52]). The deletion of the lgtB gene in Z5463BC is predicted to form a truncated LOS lacking the galactose on the terminal GlcNAc. B- Decreased size of the LOS from Z5463BC due to the loss of lgtB. Tricine SDS-PAGE gel of proteinase K LOS extracts from Z5463BC and its parental strains, revealed by silver staining. 1: Z2491 (L9 immunotype), 2: Z5463, 3: Z5463PI, 4: Z5463BC.

Mentions: The L9 immunotype lipooligosaccharide (LOS) structure of Z2491 has previously been described [52] and is schematically presented in figure 5A. According to the sequences and the apparent size of their LOS (Fig. 5B), Z5463 and Z5463PI express the same LOS as Z2491. On the other hand, Z5463BC expresses a truncated LOS as seen in figure 5B. This result is consistent with the absence of lgtB that adds a galactose on the terminal GlcNAc of the LOS. LOS structure has been described to be important for human serum resistance. We further tested the capacity of serum resistance of Z5463BC by incubating the strain with 25% of human serum. No differences were seen between Z5463BC and Z5463PI or Z5463 using commercial serum or serum isolated from the patient (data not shown). The three strains grew normally in these sera in contrast to a mutant lacking capsule which was killed by complemented serum.


Genotypic and phenotypic modifications of Neisseria meningitidis after an accidental human passage.

Omer H, Rose G, Jolley KA, Frapy E, Zahar JR, Maiden MC, Bentley SD, Tinsley CR, Nassif X, Bille E - PLoS ONE (2011)

LOS variation analysis of the strain isolated from the blood culture.A- Schematic representation of the immunotype L9 LOS of serogroup A Nm Z2491 (adapted from Zhu and coworkers [68] and Choudhury and coworkers [52]). The deletion of the lgtB gene in Z5463BC is predicted to form a truncated LOS lacking the galactose on the terminal GlcNAc. B- Decreased size of the LOS from Z5463BC due to the loss of lgtB. Tricine SDS-PAGE gel of proteinase K LOS extracts from Z5463BC and its parental strains, revealed by silver staining. 1: Z2491 (L9 immunotype), 2: Z5463, 3: Z5463PI, 4: Z5463BC.
© Copyright Policy
Related In: Results  -  Collection

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pone-0017145-g005: LOS variation analysis of the strain isolated from the blood culture.A- Schematic representation of the immunotype L9 LOS of serogroup A Nm Z2491 (adapted from Zhu and coworkers [68] and Choudhury and coworkers [52]). The deletion of the lgtB gene in Z5463BC is predicted to form a truncated LOS lacking the galactose on the terminal GlcNAc. B- Decreased size of the LOS from Z5463BC due to the loss of lgtB. Tricine SDS-PAGE gel of proteinase K LOS extracts from Z5463BC and its parental strains, revealed by silver staining. 1: Z2491 (L9 immunotype), 2: Z5463, 3: Z5463PI, 4: Z5463BC.
Mentions: The L9 immunotype lipooligosaccharide (LOS) structure of Z2491 has previously been described [52] and is schematically presented in figure 5A. According to the sequences and the apparent size of their LOS (Fig. 5B), Z5463 and Z5463PI express the same LOS as Z2491. On the other hand, Z5463BC expresses a truncated LOS as seen in figure 5B. This result is consistent with the absence of lgtB that adds a galactose on the terminal GlcNAc of the LOS. LOS structure has been described to be important for human serum resistance. We further tested the capacity of serum resistance of Z5463BC by incubating the strain with 25% of human serum. No differences were seen between Z5463BC and Z5463PI or Z5463 using commercial serum or serum isolated from the patient (data not shown). The three strains grew normally in these sera in contrast to a mutant lacking capsule which was killed by complemented serum.

Bottom Line: By comparing the number of SNP in all three isolates and knowing the number of passages between Z5463 and Z5463PI, we concluded that around 25 bacterial divisions occurred in the human body.Different pilin variants were found after the in vivo passage, which expressed different properties of adhesion.Furthermore the deletion of one gene involved in LOS biosynthesis (lgtB) results in Z5463BC expressing a different LOS than the L9 immunotype of Z2491.

View Article: PubMed Central - PubMed

Affiliation: INSERM U1002, Paris, France.

ABSTRACT
A scientist in our laboratory was accidentally infected while working with Z5463, a Neisseria meningitidis serogroup A strain. She developed severe symptoms (fever, meningism, purpuric lesions) that fortunately evolved with antibiotic treatment to complete recovery. Pulse-field gel electrophoresis confirmed that the isolate obtained from the blood culture (Z5463BC) was identical to Z5463, more precisely to a fourth subculture of this strain used the week before the contamination (Z5463PI). In order to get some insights into genomic modifications that can occur in vivo, we sequenced these three isolates. All the strains contained a mutated mutS allele and therefore displayed an hypermutator phenotype, consistent with the high number of mutations (SNP, Single Nucleotide Polymorphism) detected in the three strains. By comparing the number of SNP in all three isolates and knowing the number of passages between Z5463 and Z5463PI, we concluded that around 25 bacterial divisions occurred in the human body. As expected, the in vivo passage is responsible for several modifications of phase variable genes. This genomic study has been completed by transcriptomic and phenotypic studies, showing that the blood strain used a different haemoglobin-linked iron receptor (HpuA/B) than the parental strains (HmbR). Different pilin variants were found after the in vivo passage, which expressed different properties of adhesion. Furthermore the deletion of one gene involved in LOS biosynthesis (lgtB) results in Z5463BC expressing a different LOS than the L9 immunotype of Z2491. The in vivo passage, despite the small numbers of divisions, permits the selection of numerous genomic modifications that may account for the high capacity of the strain to disseminate.

Show MeSH
Related in: MedlinePlus