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Genotypic and phenotypic modifications of Neisseria meningitidis after an accidental human passage.

Omer H, Rose G, Jolley KA, Frapy E, Zahar JR, Maiden MC, Bentley SD, Tinsley CR, Nassif X, Bille E - PLoS ONE (2011)

Bottom Line: By comparing the number of SNP in all three isolates and knowing the number of passages between Z5463 and Z5463PI, we concluded that around 25 bacterial divisions occurred in the human body.Different pilin variants were found after the in vivo passage, which expressed different properties of adhesion.Furthermore the deletion of one gene involved in LOS biosynthesis (lgtB) results in Z5463BC expressing a different LOS than the L9 immunotype of Z2491.

View Article: PubMed Central - PubMed

Affiliation: INSERM U1002, Paris, France.

ABSTRACT
A scientist in our laboratory was accidentally infected while working with Z5463, a Neisseria meningitidis serogroup A strain. She developed severe symptoms (fever, meningism, purpuric lesions) that fortunately evolved with antibiotic treatment to complete recovery. Pulse-field gel electrophoresis confirmed that the isolate obtained from the blood culture (Z5463BC) was identical to Z5463, more precisely to a fourth subculture of this strain used the week before the contamination (Z5463PI). In order to get some insights into genomic modifications that can occur in vivo, we sequenced these three isolates. All the strains contained a mutated mutS allele and therefore displayed an hypermutator phenotype, consistent with the high number of mutations (SNP, Single Nucleotide Polymorphism) detected in the three strains. By comparing the number of SNP in all three isolates and knowing the number of passages between Z5463 and Z5463PI, we concluded that around 25 bacterial divisions occurred in the human body. As expected, the in vivo passage is responsible for several modifications of phase variable genes. This genomic study has been completed by transcriptomic and phenotypic studies, showing that the blood strain used a different haemoglobin-linked iron receptor (HpuA/B) than the parental strains (HmbR). Different pilin variants were found after the in vivo passage, which expressed different properties of adhesion. Furthermore the deletion of one gene involved in LOS biosynthesis (lgtB) results in Z5463BC expressing a different LOS than the L9 immunotype of Z2491. The in vivo passage, despite the small numbers of divisions, permits the selection of numerous genomic modifications that may account for the high capacity of the strain to disseminate.

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Related in: MedlinePlus

Distribution of the genetic differences between Z5463, Z5463PI and Z5463BC.Events are classified by 1 kb sequence with Z2491 genome as reference. Each event is represented as one coloured stretch according to the nature of the difference. The pilin region was excluded from this figure. A- Genetic differences between Z5463PI and Z5463. The novel insertion of the MDA is annotated as one stretched as it occurs at one specific position in the genome. B- Genetic differences between Z5463BC and Z5463PI. The region containing the deletion of lgtB gene is highlighted.
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pone-0017145-g004: Distribution of the genetic differences between Z5463, Z5463PI and Z5463BC.Events are classified by 1 kb sequence with Z2491 genome as reference. Each event is represented as one coloured stretch according to the nature of the difference. The pilin region was excluded from this figure. A- Genetic differences between Z5463PI and Z5463. The novel insertion of the MDA is annotated as one stretched as it occurs at one specific position in the genome. B- Genetic differences between Z5463BC and Z5463PI. The region containing the deletion of lgtB gene is highlighted.

Mentions: Unlike what has been observed between Z2491 and Z5463, comparison of Z5463, Z5463PI and Z5463BC did not reveal regions encompassing several ORFs with numerous sequence differences except for the pilin locus that is subject to antigenic variation (Fig. 4A and 4B). It should be pointed out that the pilE gene of Z5463BC could not be directly sequenced. Indeed various pilin variants were present in the DNA extracted directly from the blood culture (see below). In addition, one major difference between Z5463PI and Z5463BC was the deletion in Z5463BC of lgtB (Fig. 4B). The lgtB gene is adjacent to lgtH in Z5463 and Z5463PI and is predicted to encode a glycosyltransferase implicated in the LOS biosynthesis. Both genes have highly homologous 5′ regions. It is likely that a recombination event took place between these regions deleting the 3′ region of lgtB, the entire pseudogene lgtA' and the 5′ region of lgtH. This recombination led to the total deletion of lgtB and lgtA' and the restoration of a full lgtH gene. All other genomic differences were limited to SNP or deletions/insertion of less than 3 bp (Fig. 4, Table 1 and Table S2). 40 sequence differences were present between Z5463 and Z5463PI and 34 between Z5463PI and Z5463BC.


Genotypic and phenotypic modifications of Neisseria meningitidis after an accidental human passage.

Omer H, Rose G, Jolley KA, Frapy E, Zahar JR, Maiden MC, Bentley SD, Tinsley CR, Nassif X, Bille E - PLoS ONE (2011)

Distribution of the genetic differences between Z5463, Z5463PI and Z5463BC.Events are classified by 1 kb sequence with Z2491 genome as reference. Each event is represented as one coloured stretch according to the nature of the difference. The pilin region was excluded from this figure. A- Genetic differences between Z5463PI and Z5463. The novel insertion of the MDA is annotated as one stretched as it occurs at one specific position in the genome. B- Genetic differences between Z5463BC and Z5463PI. The region containing the deletion of lgtB gene is highlighted.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3046118&req=5

pone-0017145-g004: Distribution of the genetic differences between Z5463, Z5463PI and Z5463BC.Events are classified by 1 kb sequence with Z2491 genome as reference. Each event is represented as one coloured stretch according to the nature of the difference. The pilin region was excluded from this figure. A- Genetic differences between Z5463PI and Z5463. The novel insertion of the MDA is annotated as one stretched as it occurs at one specific position in the genome. B- Genetic differences between Z5463BC and Z5463PI. The region containing the deletion of lgtB gene is highlighted.
Mentions: Unlike what has been observed between Z2491 and Z5463, comparison of Z5463, Z5463PI and Z5463BC did not reveal regions encompassing several ORFs with numerous sequence differences except for the pilin locus that is subject to antigenic variation (Fig. 4A and 4B). It should be pointed out that the pilE gene of Z5463BC could not be directly sequenced. Indeed various pilin variants were present in the DNA extracted directly from the blood culture (see below). In addition, one major difference between Z5463PI and Z5463BC was the deletion in Z5463BC of lgtB (Fig. 4B). The lgtB gene is adjacent to lgtH in Z5463 and Z5463PI and is predicted to encode a glycosyltransferase implicated in the LOS biosynthesis. Both genes have highly homologous 5′ regions. It is likely that a recombination event took place between these regions deleting the 3′ region of lgtB, the entire pseudogene lgtA' and the 5′ region of lgtH. This recombination led to the total deletion of lgtB and lgtA' and the restoration of a full lgtH gene. All other genomic differences were limited to SNP or deletions/insertion of less than 3 bp (Fig. 4, Table 1 and Table S2). 40 sequence differences were present between Z5463 and Z5463PI and 34 between Z5463PI and Z5463BC.

Bottom Line: By comparing the number of SNP in all three isolates and knowing the number of passages between Z5463 and Z5463PI, we concluded that around 25 bacterial divisions occurred in the human body.Different pilin variants were found after the in vivo passage, which expressed different properties of adhesion.Furthermore the deletion of one gene involved in LOS biosynthesis (lgtB) results in Z5463BC expressing a different LOS than the L9 immunotype of Z2491.

View Article: PubMed Central - PubMed

Affiliation: INSERM U1002, Paris, France.

ABSTRACT
A scientist in our laboratory was accidentally infected while working with Z5463, a Neisseria meningitidis serogroup A strain. She developed severe symptoms (fever, meningism, purpuric lesions) that fortunately evolved with antibiotic treatment to complete recovery. Pulse-field gel electrophoresis confirmed that the isolate obtained from the blood culture (Z5463BC) was identical to Z5463, more precisely to a fourth subculture of this strain used the week before the contamination (Z5463PI). In order to get some insights into genomic modifications that can occur in vivo, we sequenced these three isolates. All the strains contained a mutated mutS allele and therefore displayed an hypermutator phenotype, consistent with the high number of mutations (SNP, Single Nucleotide Polymorphism) detected in the three strains. By comparing the number of SNP in all three isolates and knowing the number of passages between Z5463 and Z5463PI, we concluded that around 25 bacterial divisions occurred in the human body. As expected, the in vivo passage is responsible for several modifications of phase variable genes. This genomic study has been completed by transcriptomic and phenotypic studies, showing that the blood strain used a different haemoglobin-linked iron receptor (HpuA/B) than the parental strains (HmbR). Different pilin variants were found after the in vivo passage, which expressed different properties of adhesion. Furthermore the deletion of one gene involved in LOS biosynthesis (lgtB) results in Z5463BC expressing a different LOS than the L9 immunotype of Z2491. The in vivo passage, despite the small numbers of divisions, permits the selection of numerous genomic modifications that may account for the high capacity of the strain to disseminate.

Show MeSH
Related in: MedlinePlus