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Analysis of osmotin, a PR protein as metabolic modulator in plants.

Abdin MZ, Kiran U, Alam A - Bioinformation (2011)

Bottom Line: The results of available online DNA binding motif search programs revealed that osmotin does not contain DNAbinding motifs.However, evidence implicating osmotin in cell signaling were found during the study.With these results, we therefore, concluded that osmotin is not a transcription factor, but regulating plant responses to biotic and abiotic stresses through cell signaling.

View Article: PubMed Central - PubMed

ABSTRACT
Osmotin is an abundant cationic multifunctional protein discovered in cells of tobacco (Nicotiana tabacum L. var Wisconsin 38) adapted to an environment of low osmotic potential. Beside its role as osmoregulator, it provides plants protection from pathogens, hence also placed in the PRP family of proteins. The osmotin induced proline accumulation has been reported to confer tolerance against both biotic and abiotic stresses in plants including transgenic tomato and strawberry overexpressing osmotin gene. The exact mechanism of induction of proline by osmotin is however, not known till date. These observations have led us to hypothesize that osmotin could be regulating these plant responses through its involvement either as transcription factor, cell signal pathway modulator or both in plants. We have therefore, undertaken the present investigation to analyze the osmotin protein as transcription factor using bioinformatics tools. The results of available online DNA binding motif search programs revealed that osmotin does not contain DNAbinding motifs. The alignment results of osmotin protein with the protein sequence from DATF showed the homology in the range of 0-20%, suggesting that it might not contain a DNA binding motif. Further to find unique DNA-binding domain, the superimposition of osmotin 3D structure on modeled Arabidopsis transcription factors using Chimera also suggested absence of the same. However, evidence implicating osmotin in cell signaling were found during the study. With these results, we therefore, concluded that osmotin is not a transcription factor, but regulating plant responses to biotic and abiotic stresses through cell signaling.

No MeSH data available.


Related in: MedlinePlus

The Protein kinases signature domains present in Osmotin/thaumatin-like protein superfamily
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Related In: Results  -  Collection


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Figure 3: The Protein kinases signature domains present in Osmotin/thaumatin-like protein superfamily

Mentions: protein kinase is similar to domains found in proteins involved in cellular signalling including the catalytic domain of phosphoinositide-3-kinase (PI3K), the catalytic domain of the MHCK/EF2 kinase and choline kinase. They regulate a wide range of cellular process such as enzyme activation, assembly of macromolecules, protein localization and degradation. Three proteins in osmotin/thaumatin-like protein superfamily in A. thalianacontain this domain (AT4G18250.1, AT5G38280.1, AT1G70250) along with osmotin/thaumatin-like domain (Figure 2 and Figure 3). The third domain belongs to plant lipid-transfer and hydrophobic proteins family and is mainly involved in lipid metabolism.


Analysis of osmotin, a PR protein as metabolic modulator in plants.

Abdin MZ, Kiran U, Alam A - Bioinformation (2011)

The Protein kinases signature domains present in Osmotin/thaumatin-like protein superfamily
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3046038&req=5

Figure 3: The Protein kinases signature domains present in Osmotin/thaumatin-like protein superfamily
Mentions: protein kinase is similar to domains found in proteins involved in cellular signalling including the catalytic domain of phosphoinositide-3-kinase (PI3K), the catalytic domain of the MHCK/EF2 kinase and choline kinase. They regulate a wide range of cellular process such as enzyme activation, assembly of macromolecules, protein localization and degradation. Three proteins in osmotin/thaumatin-like protein superfamily in A. thalianacontain this domain (AT4G18250.1, AT5G38280.1, AT1G70250) along with osmotin/thaumatin-like domain (Figure 2 and Figure 3). The third domain belongs to plant lipid-transfer and hydrophobic proteins family and is mainly involved in lipid metabolism.

Bottom Line: The results of available online DNA binding motif search programs revealed that osmotin does not contain DNAbinding motifs.However, evidence implicating osmotin in cell signaling were found during the study.With these results, we therefore, concluded that osmotin is not a transcription factor, but regulating plant responses to biotic and abiotic stresses through cell signaling.

View Article: PubMed Central - PubMed

ABSTRACT
Osmotin is an abundant cationic multifunctional protein discovered in cells of tobacco (Nicotiana tabacum L. var Wisconsin 38) adapted to an environment of low osmotic potential. Beside its role as osmoregulator, it provides plants protection from pathogens, hence also placed in the PRP family of proteins. The osmotin induced proline accumulation has been reported to confer tolerance against both biotic and abiotic stresses in plants including transgenic tomato and strawberry overexpressing osmotin gene. The exact mechanism of induction of proline by osmotin is however, not known till date. These observations have led us to hypothesize that osmotin could be regulating these plant responses through its involvement either as transcription factor, cell signal pathway modulator or both in plants. We have therefore, undertaken the present investigation to analyze the osmotin protein as transcription factor using bioinformatics tools. The results of available online DNA binding motif search programs revealed that osmotin does not contain DNAbinding motifs. The alignment results of osmotin protein with the protein sequence from DATF showed the homology in the range of 0-20%, suggesting that it might not contain a DNA binding motif. Further to find unique DNA-binding domain, the superimposition of osmotin 3D structure on modeled Arabidopsis transcription factors using Chimera also suggested absence of the same. However, evidence implicating osmotin in cell signaling were found during the study. With these results, we therefore, concluded that osmotin is not a transcription factor, but regulating plant responses to biotic and abiotic stresses through cell signaling.

No MeSH data available.


Related in: MedlinePlus