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A gp63 based vaccine candidate against Visceral Leishmaniasis.

Sinha S, Sundaram S, Singh AP, Tripathi A - Bioinformation (2011)

Bottom Line: Visceral leishmaniasis is a macrophage associated disorder which leads to a profound decrease in the natural immunotherapeutic potential of the infected subjects to combat the disease.The major surface glycoprotein gp63 has been found to be a significant vaccine candidate against visceral leishmaniasis.The results from BLAST, Phylogram and Cladogram studies indicate significant identity, similarity and conservation of important residues in the protein which lead us to conclude that a common gp63 based vaccine can be used as a therapeutical tool against visceral leishmaniasis caused by different species strains of leishmania.

View Article: PubMed Central - PubMed

Affiliation: Center for Biotechnology, University of Allahabad, Allahabad, Uttar Pradesh, India-211002.

ABSTRACT
Visceral leishmaniasis is a macrophage associated disorder which leads to a profound decrease in the natural immunotherapeutic potential of the infected subjects to combat the disease. The major surface glycoprotein gp63 has been found to be a significant vaccine candidate against visceral leishmaniasis. The current study addresses the levels of similarity and identity in the gp63 obtained from different species of Leishmania viz donovoni, chagasi and infantum linked to the cause of visceral leishmaniasis. The results from BLAST, Phylogram and Cladogram studies indicate significant identity, similarity and conservation of important residues in the protein which lead us to conclude that a common gp63 based vaccine can be used as a therapeutical tool against visceral leishmaniasis caused by different species strains of leishmania.

No MeSH data available.


Related in: MedlinePlus

Cladogram and Phylogram analysis of gp63 sequences of different species strains of Leishmania associated with Visceral Leishmaniasis
© Copyright Policy - open-access
Related In: Results  -  Collection


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Figure 1: Cladogram and Phylogram analysis of gp63 sequences of different species strains of Leishmania associated with Visceral Leishmaniasis

Mentions: BLAST (Table 1 see Table 1) shows a high level of similarity and identity almost in the range of more than 90% and negligible percentage of gaps among the amino acid residues of the gp63 molecule. T-COFFEE (Figure 2) results show high level of identity, similarity and positives almost towards Good then towards Average and least towards Bad which again strongly advocate the high level of conservation with in aminoacid residues of gp3. CLADOGRAM and PHYLOGRAM (Figure 1) analysis also show tight vicinity among the gp63 residues during the process of evolution since the nodes are very close to each other. The Cladogram is lower in length, it has fewer homoplasies and it is more parsimonious. CAB51784.1 and CAB42815.1 are more closely related and both of them are close towards CAB42816.1. Moreover CAB51783.1 is related to all three. In the other branch CAB51797.2 and CAB51794.1 are closely related. They are further close to CAB51793.1. These gp63 proteins obtained from seven different species strains of Leishmania are more closely related amongst themselves as they arose from gene duplication. Moreover the sequence CAB06018.1 has altogether a different branch and it has relatively much less similarity with other gp3 proteins. The scale of Phylogram is 0.3 and it also strongly supports the results of Cladogram analysis.


A gp63 based vaccine candidate against Visceral Leishmaniasis.

Sinha S, Sundaram S, Singh AP, Tripathi A - Bioinformation (2011)

Cladogram and Phylogram analysis of gp63 sequences of different species strains of Leishmania associated with Visceral Leishmaniasis
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3046035&req=5

Figure 1: Cladogram and Phylogram analysis of gp63 sequences of different species strains of Leishmania associated with Visceral Leishmaniasis
Mentions: BLAST (Table 1 see Table 1) shows a high level of similarity and identity almost in the range of more than 90% and negligible percentage of gaps among the amino acid residues of the gp63 molecule. T-COFFEE (Figure 2) results show high level of identity, similarity and positives almost towards Good then towards Average and least towards Bad which again strongly advocate the high level of conservation with in aminoacid residues of gp3. CLADOGRAM and PHYLOGRAM (Figure 1) analysis also show tight vicinity among the gp63 residues during the process of evolution since the nodes are very close to each other. The Cladogram is lower in length, it has fewer homoplasies and it is more parsimonious. CAB51784.1 and CAB42815.1 are more closely related and both of them are close towards CAB42816.1. Moreover CAB51783.1 is related to all three. In the other branch CAB51797.2 and CAB51794.1 are closely related. They are further close to CAB51793.1. These gp63 proteins obtained from seven different species strains of Leishmania are more closely related amongst themselves as they arose from gene duplication. Moreover the sequence CAB06018.1 has altogether a different branch and it has relatively much less similarity with other gp3 proteins. The scale of Phylogram is 0.3 and it also strongly supports the results of Cladogram analysis.

Bottom Line: Visceral leishmaniasis is a macrophage associated disorder which leads to a profound decrease in the natural immunotherapeutic potential of the infected subjects to combat the disease.The major surface glycoprotein gp63 has been found to be a significant vaccine candidate against visceral leishmaniasis.The results from BLAST, Phylogram and Cladogram studies indicate significant identity, similarity and conservation of important residues in the protein which lead us to conclude that a common gp63 based vaccine can be used as a therapeutical tool against visceral leishmaniasis caused by different species strains of leishmania.

View Article: PubMed Central - PubMed

Affiliation: Center for Biotechnology, University of Allahabad, Allahabad, Uttar Pradesh, India-211002.

ABSTRACT
Visceral leishmaniasis is a macrophage associated disorder which leads to a profound decrease in the natural immunotherapeutic potential of the infected subjects to combat the disease. The major surface glycoprotein gp63 has been found to be a significant vaccine candidate against visceral leishmaniasis. The current study addresses the levels of similarity and identity in the gp63 obtained from different species of Leishmania viz donovoni, chagasi and infantum linked to the cause of visceral leishmaniasis. The results from BLAST, Phylogram and Cladogram studies indicate significant identity, similarity and conservation of important residues in the protein which lead us to conclude that a common gp63 based vaccine can be used as a therapeutical tool against visceral leishmaniasis caused by different species strains of leishmania.

No MeSH data available.


Related in: MedlinePlus