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Olive oil effectively mitigates ovariectomy-induced osteoporosis in rats.

Saleh NK, Saleh HA - BMC Complement Altern Med (2011)

Bottom Line: The OVX-rats showed a significant decrease in plasma calcium levels, and a significant increase in plasma ALP, MDA, and nitrates levels.Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT).In addition, there was a significant increase in the osteoclast number denoting bone resorption.

View Article: PubMed Central - HTML - PubMed

Affiliation: Faculty of Medicine, Ain Shams University, Physiology Department, Egypt. nermine_saleh@yahoo.com.

ABSTRACT

Background: Osteoporosis, a reduction in bone mineral density, represents the most common metabolic bone disease. Postmenopausal women are particularly susceptible to osteoporosis when their production of estrogen declines. For these women, fracture is a leading cause of morbidity and mortality. This study was conducted to evaluate the protective effects of olive oil supplementation against osteoporosis in ovariectomized (OVX) rats.

Methods: We studied adult female Wistar rats aged 12-14 months, divided into three groups: sham-operated control (SHAM), ovariectomized (OVX), and ovariectomized rats supplemented with extravirgin olive oil (Olive-OVX) orally for 12 weeks; 4 weeks before ovariectomy and 8 weeks after. At the end of the experiment, blood samples were collected. Plasma levels of calcium, phosphorus, alkaline phosphatase (ALP), malondialdehyde (MDA), and nitrates were assayed. Specimens from both the tibia and the liver were processed for light microscopic examination. Histomorphometric analysis of the tibia was also performed.

Results: The OVX-rats showed a significant decrease in plasma calcium levels, and a significant increase in plasma ALP, MDA, and nitrates levels. These changes were attenuated by olive oil supplementation in the Olive-OVX rats. Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT). In addition, there was a significant increase in the osteoclast number denoting bone resorption. In the Olive-OVX rats these parameters were markedly improved as compared to the OVX group. Examination of the liver specimens revealed mononuclear cellular infiltration in the portal areas in the OVX-rats which was not detected in the Olive-OVX rats.

Conclusions: Olive oil effectively mitigated ovariectomy-induced osteoporosis in rats, and is a promising candidate for the treatment of postmenopausal osteoporosis.

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Proximal metaphysis of tibia of the OVX-group (H&E). A: loss of the normal architecture of trabeculae of cancellous bone with widening of bone marrow spaces (×200). B: apparent increase in the number of osteoclasts (OCL).(×640) C: higher magnification of multinucleated osteoclast (OCL) in Howship's lacunae (×1000).
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Figure 3: Proximal metaphysis of tibia of the OVX-group (H&E). A: loss of the normal architecture of trabeculae of cancellous bone with widening of bone marrow spaces (×200). B: apparent increase in the number of osteoclasts (OCL).(×640) C: higher magnification of multinucleated osteoclast (OCL) in Howship's lacunae (×1000).

Mentions: Bone sections in the OVX-rats revealed thinning of the outer cortical bone as compared to the control group. The inner cancellous bone trabeculae lost their normal architecture and appeared as discontinuous bony ossicles separated by widened bone marrow spaces (Figure 3A, 4A). Osteoclasts were apparently increased as compared to the SHAM- operated control group (Figure 3B, C, 4C). Erosion cavities were detected in the endosteal surface in some trabeculae and proliferation of osteoblasts was also detected in some areas (Figure 4B). The Mean CBT and the mean TBT showed a significant decrease as compared to the SHAM- operated control group whereas the osteoclast number was significantly increased (Table 3).


Olive oil effectively mitigates ovariectomy-induced osteoporosis in rats.

Saleh NK, Saleh HA - BMC Complement Altern Med (2011)

Proximal metaphysis of tibia of the OVX-group (H&E). A: loss of the normal architecture of trabeculae of cancellous bone with widening of bone marrow spaces (×200). B: apparent increase in the number of osteoclasts (OCL).(×640) C: higher magnification of multinucleated osteoclast (OCL) in Howship's lacunae (×1000).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3045997&req=5

Figure 3: Proximal metaphysis of tibia of the OVX-group (H&E). A: loss of the normal architecture of trabeculae of cancellous bone with widening of bone marrow spaces (×200). B: apparent increase in the number of osteoclasts (OCL).(×640) C: higher magnification of multinucleated osteoclast (OCL) in Howship's lacunae (×1000).
Mentions: Bone sections in the OVX-rats revealed thinning of the outer cortical bone as compared to the control group. The inner cancellous bone trabeculae lost their normal architecture and appeared as discontinuous bony ossicles separated by widened bone marrow spaces (Figure 3A, 4A). Osteoclasts were apparently increased as compared to the SHAM- operated control group (Figure 3B, C, 4C). Erosion cavities were detected in the endosteal surface in some trabeculae and proliferation of osteoblasts was also detected in some areas (Figure 4B). The Mean CBT and the mean TBT showed a significant decrease as compared to the SHAM- operated control group whereas the osteoclast number was significantly increased (Table 3).

Bottom Line: The OVX-rats showed a significant decrease in plasma calcium levels, and a significant increase in plasma ALP, MDA, and nitrates levels.Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT).In addition, there was a significant increase in the osteoclast number denoting bone resorption.

View Article: PubMed Central - HTML - PubMed

Affiliation: Faculty of Medicine, Ain Shams University, Physiology Department, Egypt. nermine_saleh@yahoo.com.

ABSTRACT

Background: Osteoporosis, a reduction in bone mineral density, represents the most common metabolic bone disease. Postmenopausal women are particularly susceptible to osteoporosis when their production of estrogen declines. For these women, fracture is a leading cause of morbidity and mortality. This study was conducted to evaluate the protective effects of olive oil supplementation against osteoporosis in ovariectomized (OVX) rats.

Methods: We studied adult female Wistar rats aged 12-14 months, divided into three groups: sham-operated control (SHAM), ovariectomized (OVX), and ovariectomized rats supplemented with extravirgin olive oil (Olive-OVX) orally for 12 weeks; 4 weeks before ovariectomy and 8 weeks after. At the end of the experiment, blood samples were collected. Plasma levels of calcium, phosphorus, alkaline phosphatase (ALP), malondialdehyde (MDA), and nitrates were assayed. Specimens from both the tibia and the liver were processed for light microscopic examination. Histomorphometric analysis of the tibia was also performed.

Results: The OVX-rats showed a significant decrease in plasma calcium levels, and a significant increase in plasma ALP, MDA, and nitrates levels. These changes were attenuated by olive oil supplementation in the Olive-OVX rats. Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT). In addition, there was a significant increase in the osteoclast number denoting bone resorption. In the Olive-OVX rats these parameters were markedly improved as compared to the OVX group. Examination of the liver specimens revealed mononuclear cellular infiltration in the portal areas in the OVX-rats which was not detected in the Olive-OVX rats.

Conclusions: Olive oil effectively mitigated ovariectomy-induced osteoporosis in rats, and is a promising candidate for the treatment of postmenopausal osteoporosis.

Show MeSH
Related in: MedlinePlus