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Olive oil effectively mitigates ovariectomy-induced osteoporosis in rats.

Saleh NK, Saleh HA - BMC Complement Altern Med (2011)

Bottom Line: The OVX-rats showed a significant decrease in plasma calcium levels, and a significant increase in plasma ALP, MDA, and nitrates levels.Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT).In addition, there was a significant increase in the osteoclast number denoting bone resorption.

View Article: PubMed Central - HTML - PubMed

Affiliation: Faculty of Medicine, Ain Shams University, Physiology Department, Egypt. nermine_saleh@yahoo.com.

ABSTRACT

Background: Osteoporosis, a reduction in bone mineral density, represents the most common metabolic bone disease. Postmenopausal women are particularly susceptible to osteoporosis when their production of estrogen declines. For these women, fracture is a leading cause of morbidity and mortality. This study was conducted to evaluate the protective effects of olive oil supplementation against osteoporosis in ovariectomized (OVX) rats.

Methods: We studied adult female Wistar rats aged 12-14 months, divided into three groups: sham-operated control (SHAM), ovariectomized (OVX), and ovariectomized rats supplemented with extravirgin olive oil (Olive-OVX) orally for 12 weeks; 4 weeks before ovariectomy and 8 weeks after. At the end of the experiment, blood samples were collected. Plasma levels of calcium, phosphorus, alkaline phosphatase (ALP), malondialdehyde (MDA), and nitrates were assayed. Specimens from both the tibia and the liver were processed for light microscopic examination. Histomorphometric analysis of the tibia was also performed.

Results: The OVX-rats showed a significant decrease in plasma calcium levels, and a significant increase in plasma ALP, MDA, and nitrates levels. These changes were attenuated by olive oil supplementation in the Olive-OVX rats. Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT). In addition, there was a significant increase in the osteoclast number denoting bone resorption. In the Olive-OVX rats these parameters were markedly improved as compared to the OVX group. Examination of the liver specimens revealed mononuclear cellular infiltration in the portal areas in the OVX-rats which was not detected in the Olive-OVX rats.

Conclusions: Olive oil effectively mitigated ovariectomy-induced osteoporosis in rats, and is a promising candidate for the treatment of postmenopausal osteoporosis.

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Related in: MedlinePlus

Proximal metaphysis of tibia of the control group (Azure II-Methylene blue). A: bone trabeculae (black triangle) with bone marrow spaces (m) inbetween (×250). B: osteoprogenitor cells (op), and C: osteoblasts (ob) line the endosteal surface. (×640).
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Figure 2: Proximal metaphysis of tibia of the control group (Azure II-Methylene blue). A: bone trabeculae (black triangle) with bone marrow spaces (m) inbetween (×250). B: osteoprogenitor cells (op), and C: osteoblasts (ob) line the endosteal surface. (×640).

Mentions: Cancellous bone was formed of a network of bone trabeculae composed of irregular bone lamellae between which osteocytes appeared in their lacunae (Figure 1A, 2A). The endosteal surface of trabeculae was lined by osteoprogenitor cells (Figure 1B, 2B), osteoblasts (Figure 1C, 2C) and osteoclasts in Howship's lacunae (Figure 1D). Bone marrow spaces were seen between the trabeculae (Figure 1A, 2A).


Olive oil effectively mitigates ovariectomy-induced osteoporosis in rats.

Saleh NK, Saleh HA - BMC Complement Altern Med (2011)

Proximal metaphysis of tibia of the control group (Azure II-Methylene blue). A: bone trabeculae (black triangle) with bone marrow spaces (m) inbetween (×250). B: osteoprogenitor cells (op), and C: osteoblasts (ob) line the endosteal surface. (×640).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3045997&req=5

Figure 2: Proximal metaphysis of tibia of the control group (Azure II-Methylene blue). A: bone trabeculae (black triangle) with bone marrow spaces (m) inbetween (×250). B: osteoprogenitor cells (op), and C: osteoblasts (ob) line the endosteal surface. (×640).
Mentions: Cancellous bone was formed of a network of bone trabeculae composed of irregular bone lamellae between which osteocytes appeared in their lacunae (Figure 1A, 2A). The endosteal surface of trabeculae was lined by osteoprogenitor cells (Figure 1B, 2B), osteoblasts (Figure 1C, 2C) and osteoclasts in Howship's lacunae (Figure 1D). Bone marrow spaces were seen between the trabeculae (Figure 1A, 2A).

Bottom Line: The OVX-rats showed a significant decrease in plasma calcium levels, and a significant increase in plasma ALP, MDA, and nitrates levels.Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT).In addition, there was a significant increase in the osteoclast number denoting bone resorption.

View Article: PubMed Central - HTML - PubMed

Affiliation: Faculty of Medicine, Ain Shams University, Physiology Department, Egypt. nermine_saleh@yahoo.com.

ABSTRACT

Background: Osteoporosis, a reduction in bone mineral density, represents the most common metabolic bone disease. Postmenopausal women are particularly susceptible to osteoporosis when their production of estrogen declines. For these women, fracture is a leading cause of morbidity and mortality. This study was conducted to evaluate the protective effects of olive oil supplementation against osteoporosis in ovariectomized (OVX) rats.

Methods: We studied adult female Wistar rats aged 12-14 months, divided into three groups: sham-operated control (SHAM), ovariectomized (OVX), and ovariectomized rats supplemented with extravirgin olive oil (Olive-OVX) orally for 12 weeks; 4 weeks before ovariectomy and 8 weeks after. At the end of the experiment, blood samples were collected. Plasma levels of calcium, phosphorus, alkaline phosphatase (ALP), malondialdehyde (MDA), and nitrates were assayed. Specimens from both the tibia and the liver were processed for light microscopic examination. Histomorphometric analysis of the tibia was also performed.

Results: The OVX-rats showed a significant decrease in plasma calcium levels, and a significant increase in plasma ALP, MDA, and nitrates levels. These changes were attenuated by olive oil supplementation in the Olive-OVX rats. Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT). In addition, there was a significant increase in the osteoclast number denoting bone resorption. In the Olive-OVX rats these parameters were markedly improved as compared to the OVX group. Examination of the liver specimens revealed mononuclear cellular infiltration in the portal areas in the OVX-rats which was not detected in the Olive-OVX rats.

Conclusions: Olive oil effectively mitigated ovariectomy-induced osteoporosis in rats, and is a promising candidate for the treatment of postmenopausal osteoporosis.

Show MeSH
Related in: MedlinePlus