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Overexpression of LCMR1 is significantly associated with clinical stage in human NSCLC.

Chen L, Liang Z, Tian Q, Li C, Ma X, Zhang Y, Yang Z, Wang P, Li Y - J. Exp. Clin. Cancer Res. (2011)

Bottom Line: Differential Display-PCR technique was used to achieve the novel cDNA, which were then verified by real-time PCR.LCMR1 was differentially expressed in different human tissues.LCMR1 was strongly overexpressed in NSCLC and its expression was significantly associated with clinical stage.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Respiratory Diseases, Chinese PLA General Hospital, Beijing 100853, PR China. chenliangan301@163.com

ABSTRACT

Background: Lung cancer is one of the most common human cancers and the leading cause of cancer death worldwide. The identification of lung cancer associated genes is essential for lung cancer diagnosis and treatment.

Methods: Differential Display-PCR technique was used to achieve the novel cDNA, which were then verified by real-time PCR. Northern blot was utilized to observe the expression of LCMR1 in different human tissues. 84 cases human NSCLC tissues and normal counterparts were analyzed for the expression of LCMR1 by immunohistochemistry.

Results: A novel 778-bp cDNA fragment from human large cell lung carcinoma cell lines 95C and 95D was obtained, and named LCMR1 (Lung Cancer Metastasis Related protein 1). LCMR1 was differentially expressed in different human tissues. LCMR1 was strongly overexpressed in NSCLC and its expression was significantly associated with clinical stage.

Conclusion: Our data indicated that LCMR1, strongly overexpressed in NSCLC, might have applications in the clinical diagnosis and treatment of lung cancer.

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Related in: MedlinePlus

Kaplan-Meier analysis of 65 cases follow-up. The survival curve showed increased difference in survival rates between patients with high-level LCMR1 protein expression and patients with low-level LCMR1 expression, with overall survival time extension.
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Figure 4: Kaplan-Meier analysis of 65 cases follow-up. The survival curve showed increased difference in survival rates between patients with high-level LCMR1 protein expression and patients with low-level LCMR1 expression, with overall survival time extension.

Mentions: Kaplan-Meier analysis of 65 cases of this group, with a median follow-up of 31 months, showed increased difference in survival rates between patients with high-level LCMR1 protein expression and patients with low-level LCMR1 expression, with overall survival time extension (Figure 4). But no statistical significance was observed in overall survival (OS) and progression-free survival (PFS) of these NSCLC patients using univariate survival analysis and multivariate survival analysis and COX proportional hazard model analysis (data not shown).


Overexpression of LCMR1 is significantly associated with clinical stage in human NSCLC.

Chen L, Liang Z, Tian Q, Li C, Ma X, Zhang Y, Yang Z, Wang P, Li Y - J. Exp. Clin. Cancer Res. (2011)

Kaplan-Meier analysis of 65 cases follow-up. The survival curve showed increased difference in survival rates between patients with high-level LCMR1 protein expression and patients with low-level LCMR1 expression, with overall survival time extension.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3045976&req=5

Figure 4: Kaplan-Meier analysis of 65 cases follow-up. The survival curve showed increased difference in survival rates between patients with high-level LCMR1 protein expression and patients with low-level LCMR1 expression, with overall survival time extension.
Mentions: Kaplan-Meier analysis of 65 cases of this group, with a median follow-up of 31 months, showed increased difference in survival rates between patients with high-level LCMR1 protein expression and patients with low-level LCMR1 expression, with overall survival time extension (Figure 4). But no statistical significance was observed in overall survival (OS) and progression-free survival (PFS) of these NSCLC patients using univariate survival analysis and multivariate survival analysis and COX proportional hazard model analysis (data not shown).

Bottom Line: Differential Display-PCR technique was used to achieve the novel cDNA, which were then verified by real-time PCR.LCMR1 was differentially expressed in different human tissues.LCMR1 was strongly overexpressed in NSCLC and its expression was significantly associated with clinical stage.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Respiratory Diseases, Chinese PLA General Hospital, Beijing 100853, PR China. chenliangan301@163.com

ABSTRACT

Background: Lung cancer is one of the most common human cancers and the leading cause of cancer death worldwide. The identification of lung cancer associated genes is essential for lung cancer diagnosis and treatment.

Methods: Differential Display-PCR technique was used to achieve the novel cDNA, which were then verified by real-time PCR. Northern blot was utilized to observe the expression of LCMR1 in different human tissues. 84 cases human NSCLC tissues and normal counterparts were analyzed for the expression of LCMR1 by immunohistochemistry.

Results: A novel 778-bp cDNA fragment from human large cell lung carcinoma cell lines 95C and 95D was obtained, and named LCMR1 (Lung Cancer Metastasis Related protein 1). LCMR1 was differentially expressed in different human tissues. LCMR1 was strongly overexpressed in NSCLC and its expression was significantly associated with clinical stage.

Conclusion: Our data indicated that LCMR1, strongly overexpressed in NSCLC, might have applications in the clinical diagnosis and treatment of lung cancer.

Show MeSH
Related in: MedlinePlus