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Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencing.

Shin H, Lee H, Fejes AP, Baillie DL, Koo HS, Jones SJ - BMC Res Notes (2011)

Bottom Line: Specifically, we compared the transcriptomes of aak-2 and wild type animals under normal conditions and conditions of induced oxidative stress.The analysis presented in this study has enabled us to identify potential genes involved in stress resistance that may be either directly or indirectly under the control of AAK-2.Furthermore, we have extended our current knowledge of general defense responses of C. elegans against oxidative stress supporting the function for AAK-2 in inhibition of biosynthetic processes, especially lipid synthesis, under oxidative stress and transcriptional regulation of genes involved in reproductive processes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Genome Sciences Centre, BC Cancer Agency, Suite 100 570 West 7th Avenue, Vancouver, British Columbia, Canada V5Z 4S6. sjones@bcgsc.ca.

ABSTRACT

Background: A strong association between stress resistance and longevity in multicellular organisms has been established as many mutations that extend lifespan also show increased resistance to stress. AAK-2, the C. elegans homolog of an alpha subunit of AMP-activated protein kinase (AMPK) is an intracellular fuel sensor that regulates cellular energy homeostasis and functions in stress resistance and lifespan extension.

Findings: Here, we investigated global transcriptional responses of aak-2 mutants to oxidative stress and in turn identified potential downstream targets of AAK-2 involved in stress resistance in C. elegans. We employed massively parallel Illumina sequencing technology and performed comprehensive comparative transcriptome analysis. Specifically, we compared the transcriptomes of aak-2 and wild type animals under normal conditions and conditions of induced oxidative stress. This research has presented a snapshot of genome-wide transcriptional activities that take place in C. elegans in response to oxidative stress both in the presence and absence of AAK-2.

Conclusions: The analysis presented in this study has enabled us to identify potential genes involved in stress resistance that may be either directly or indirectly under the control of AAK-2. Furthermore, we have extended our current knowledge of general defense responses of C. elegans against oxidative stress supporting the function for AAK-2 in inhibition of biosynthetic processes, especially lipid synthesis, under oxidative stress and transcriptional regulation of genes involved in reproductive processes.

No MeSH data available.


Related in: MedlinePlus

Genes with statistically significant differential gene expression. Number of genes significantly up or down-regulated in stressed wild type, stressed aak-2 mutants, and unstressed aak-2 mutants relative to unstressed wild type used as a control. There are considerably more genes up or down-regulated in stressed and unstressed aak-2 mutants than stressed wild type.
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Figure 1: Genes with statistically significant differential gene expression. Number of genes significantly up or down-regulated in stressed wild type, stressed aak-2 mutants, and unstressed aak-2 mutants relative to unstressed wild type used as a control. There are considerably more genes up or down-regulated in stressed and unstressed aak-2 mutants than stressed wild type.

Mentions: We first examined the transcriptional changes in wild type animals after exposure to paraquat induced oxidative stress. Using the statistical method discussed above, we identified 499 genes whose expression was significantly changed. Of the 499 differentially expressed genes, 323 were up-regulated and 176 down-regulated (Figure 1). In order to identify over represented functions associated with the differentially expressed genes, we used GOstat [22], a tool to categorize gene ontology annotations and generate the statistics of over represented terms.


Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencing.

Shin H, Lee H, Fejes AP, Baillie DL, Koo HS, Jones SJ - BMC Res Notes (2011)

Genes with statistically significant differential gene expression. Number of genes significantly up or down-regulated in stressed wild type, stressed aak-2 mutants, and unstressed aak-2 mutants relative to unstressed wild type used as a control. There are considerably more genes up or down-regulated in stressed and unstressed aak-2 mutants than stressed wild type.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3045954&req=5

Figure 1: Genes with statistically significant differential gene expression. Number of genes significantly up or down-regulated in stressed wild type, stressed aak-2 mutants, and unstressed aak-2 mutants relative to unstressed wild type used as a control. There are considerably more genes up or down-regulated in stressed and unstressed aak-2 mutants than stressed wild type.
Mentions: We first examined the transcriptional changes in wild type animals after exposure to paraquat induced oxidative stress. Using the statistical method discussed above, we identified 499 genes whose expression was significantly changed. Of the 499 differentially expressed genes, 323 were up-regulated and 176 down-regulated (Figure 1). In order to identify over represented functions associated with the differentially expressed genes, we used GOstat [22], a tool to categorize gene ontology annotations and generate the statistics of over represented terms.

Bottom Line: Specifically, we compared the transcriptomes of aak-2 and wild type animals under normal conditions and conditions of induced oxidative stress.The analysis presented in this study has enabled us to identify potential genes involved in stress resistance that may be either directly or indirectly under the control of AAK-2.Furthermore, we have extended our current knowledge of general defense responses of C. elegans against oxidative stress supporting the function for AAK-2 in inhibition of biosynthetic processes, especially lipid synthesis, under oxidative stress and transcriptional regulation of genes involved in reproductive processes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Genome Sciences Centre, BC Cancer Agency, Suite 100 570 West 7th Avenue, Vancouver, British Columbia, Canada V5Z 4S6. sjones@bcgsc.ca.

ABSTRACT

Background: A strong association between stress resistance and longevity in multicellular organisms has been established as many mutations that extend lifespan also show increased resistance to stress. AAK-2, the C. elegans homolog of an alpha subunit of AMP-activated protein kinase (AMPK) is an intracellular fuel sensor that regulates cellular energy homeostasis and functions in stress resistance and lifespan extension.

Findings: Here, we investigated global transcriptional responses of aak-2 mutants to oxidative stress and in turn identified potential downstream targets of AAK-2 involved in stress resistance in C. elegans. We employed massively parallel Illumina sequencing technology and performed comprehensive comparative transcriptome analysis. Specifically, we compared the transcriptomes of aak-2 and wild type animals under normal conditions and conditions of induced oxidative stress. This research has presented a snapshot of genome-wide transcriptional activities that take place in C. elegans in response to oxidative stress both in the presence and absence of AAK-2.

Conclusions: The analysis presented in this study has enabled us to identify potential genes involved in stress resistance that may be either directly or indirectly under the control of AAK-2. Furthermore, we have extended our current knowledge of general defense responses of C. elegans against oxidative stress supporting the function for AAK-2 in inhibition of biosynthetic processes, especially lipid synthesis, under oxidative stress and transcriptional regulation of genes involved in reproductive processes.

No MeSH data available.


Related in: MedlinePlus