Hybridization and restricted gene flow between native and introduced stocks of Alpine whitefish (Coregonus sp.) across multiple environments.
Bottom Line: Fifteen populations (N=747) were assessed for allelic variation at eight microsatellite loci and a reduced set (N=253) for variation across two mtDNA genes (cyt b and NADH-3).Although statistically congruent with inferences based on nuclear markers, mitochondrial haplotype data was not diagnostic with respect to native and non-native lineages, supporting that the Alpine region was colonized post-glacially by an admixture of mtDNA lineages, which coalesce >1 Ma.Mechanisms promoting or eroding lineage isolation are discussed, as well as a high potential to conserve native Alpine lineages despite the extensive historical use of introduced Baltic stocks.
Affiliation: Institute for Zoology, Karl-Franzens University Graz, Universitätsplatz 2, Graz, Austria.Show MeSH
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Mentions: Adult fish were sampled from 12 Austrian lakes using monofilament gillnets. All Austrian populations belong to the Danube drainage, and to our knowledge only one of them (Achensee) has been integrated into previous studies (Douglas & Brunner 2002). Three additional samples of Baltic origin included the so-called ‘hatchery Maräne’ (hence referred to as OU-introduced) and two wild-caught samples from coastal waters in Germany and Poland (Table 1; Fig. 1). The OU-introduced is a commercial stock reared for decades in the Waldviertel region of Austria and widely used for management throughout the country. Larvae from one additional Austrian lake (Traunsee) were included for some limited genetic analysis (mtDNA). Fin clips were preserved in 95% ethanol and total genomic DNA was extracted using a high-salt (ammonium acetate) extraction protocol modified from Miller et al. (1988).
Affiliation: Institute for Zoology, Karl-Franzens University Graz, Universitätsplatz 2, Graz, Austria.