Expression of pleiotrophin in the prostate is androgen regulated and it functions as an autocrine regulator of mesenchyme and cancer associated fibroblasts and as a paracrine regulator of epithelia.
Bottom Line: Ptn transcripts and protein were localized by in situ and immunohistochemistry and Ptn mRNA was quantitated by Northern blot and qRT-PCR.PTN also showed male enriched expression in fetal human male urethra versus female, and between wt male and ARKO male mice.Ptn protein was increased by testosterone in organ cultures of female rat VMP and in rat male urethra compared to female.
Affiliation: MRC Human Reproductive Sciences Unit, The Queen's Medical Research Institute, Edinburgh, UK.Show MeSH
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Mentions: To examine the effect of hPTN upon the cellular differentiation and proliferation of VPs grown in vitro (n = 3, 27 organs) we examined the organs by histology and calculated proliferative rates using immunohistochemistry for BrdU incorporation and stromal and epithelial markers (Fig. 4). Histology of the VP organs showed no substantial changes in organ morphology or stromal and epithelial distribution (Fig. 4A). Analysis of stromal and epithelial differentiation using SM α-actin and p63 showed no difference in cellular differentiation after treatment with hPTN, but confirmed increased branching of ductal tips at the periphery of the VP organs (Supplementary Fig. 2). The proliferative index of the epithelial and mesenchymal compartments in the peripheral region (distal to the urethra) was calculated for VPs cultured ±T, ±hPTN (Fig. 4B,C and values are listed in Supplementary Tables C and D). hPTN significantly increased epithelial (P < 0.05) and stromal (P < 0.01) proliferation in the absence of testosterone.
Affiliation: MRC Human Reproductive Sciences Unit, The Queen's Medical Research Institute, Edinburgh, UK.