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Expression of pleiotrophin in the prostate is androgen regulated and it functions as an autocrine regulator of mesenchyme and cancer associated fibroblasts and as a paracrine regulator of epithelia.

Orr B, Vanpoucke G, Grace OC, Smith L, Anderson RA, Riddick AC, Franco OE, Hayward SW, Thomson AA - Prostate (2010)

Bottom Line: Ptn transcripts and protein were localized by in situ and immunohistochemistry and Ptn mRNA was quantitated by Northern blot and qRT-PCR.PTN also showed male enriched expression in fetal human male urethra versus female, and between wt male and ARKO male mice.Ptn protein was increased by testosterone in organ cultures of female rat VMP and in rat male urethra compared to female.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, The Queen's Medical Research Institute, Edinburgh, UK.

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Related in: MedlinePlus

The effect of hPTN on cellular proliferation in the proximal and distal regions of the prostate. Rat VPs (P0) were grown ±T and ±hPTN for 6 days and BrdU was added to the culture medium for 2 hr prior to harvesting. Panel A: Histology (hematoxylin and eosin staining) of cultured VPs after 6 days, asterisks (*) mark epithelial buds at the periphery of the organ. Scale bar represents 100 µm. Panel B: Immunohistochemistry of VP epithelial ducts distal to the urethra under different treatment conditions showing BrdU incorporation (green) in the epithelial cells (red); nuclei were stained with Topro (blue). Scale bar represents 100 µm. Panel C: Graphs of BrdU incorporation ±SEM illustrating the rates of proliferation of stroma and epithelia. Addition of hPTN led to an increase in stromal and epithelial cell proliferation only in the absence of testosterone (Student's t-test, **P < 0.01).
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fig04: The effect of hPTN on cellular proliferation in the proximal and distal regions of the prostate. Rat VPs (P0) were grown ±T and ±hPTN for 6 days and BrdU was added to the culture medium for 2 hr prior to harvesting. Panel A: Histology (hematoxylin and eosin staining) of cultured VPs after 6 days, asterisks (*) mark epithelial buds at the periphery of the organ. Scale bar represents 100 µm. Panel B: Immunohistochemistry of VP epithelial ducts distal to the urethra under different treatment conditions showing BrdU incorporation (green) in the epithelial cells (red); nuclei were stained with Topro (blue). Scale bar represents 100 µm. Panel C: Graphs of BrdU incorporation ±SEM illustrating the rates of proliferation of stroma and epithelia. Addition of hPTN led to an increase in stromal and epithelial cell proliferation only in the absence of testosterone (Student's t-test, **P < 0.01).

Mentions: To examine the effect of hPTN upon the cellular differentiation and proliferation of VPs grown in vitro (n = 3, 27 organs) we examined the organs by histology and calculated proliferative rates using immunohistochemistry for BrdU incorporation and stromal and epithelial markers (Fig. 4). Histology of the VP organs showed no substantial changes in organ morphology or stromal and epithelial distribution (Fig. 4A). Analysis of stromal and epithelial differentiation using SM α-actin and p63 showed no difference in cellular differentiation after treatment with hPTN, but confirmed increased branching of ductal tips at the periphery of the VP organs (Supplementary Fig. 2). The proliferative index of the epithelial and mesenchymal compartments in the peripheral region (distal to the urethra) was calculated for VPs cultured ±T, ±hPTN (Fig. 4B,C and values are listed in Supplementary Tables C and D). hPTN significantly increased epithelial (P < 0.05) and stromal (P < 0.01) proliferation in the absence of testosterone.


Expression of pleiotrophin in the prostate is androgen regulated and it functions as an autocrine regulator of mesenchyme and cancer associated fibroblasts and as a paracrine regulator of epithelia.

Orr B, Vanpoucke G, Grace OC, Smith L, Anderson RA, Riddick AC, Franco OE, Hayward SW, Thomson AA - Prostate (2010)

The effect of hPTN on cellular proliferation in the proximal and distal regions of the prostate. Rat VPs (P0) were grown ±T and ±hPTN for 6 days and BrdU was added to the culture medium for 2 hr prior to harvesting. Panel A: Histology (hematoxylin and eosin staining) of cultured VPs after 6 days, asterisks (*) mark epithelial buds at the periphery of the organ. Scale bar represents 100 µm. Panel B: Immunohistochemistry of VP epithelial ducts distal to the urethra under different treatment conditions showing BrdU incorporation (green) in the epithelial cells (red); nuclei were stained with Topro (blue). Scale bar represents 100 µm. Panel C: Graphs of BrdU incorporation ±SEM illustrating the rates of proliferation of stroma and epithelia. Addition of hPTN led to an increase in stromal and epithelial cell proliferation only in the absence of testosterone (Student's t-test, **P < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3045659&req=5

fig04: The effect of hPTN on cellular proliferation in the proximal and distal regions of the prostate. Rat VPs (P0) were grown ±T and ±hPTN for 6 days and BrdU was added to the culture medium for 2 hr prior to harvesting. Panel A: Histology (hematoxylin and eosin staining) of cultured VPs after 6 days, asterisks (*) mark epithelial buds at the periphery of the organ. Scale bar represents 100 µm. Panel B: Immunohistochemistry of VP epithelial ducts distal to the urethra under different treatment conditions showing BrdU incorporation (green) in the epithelial cells (red); nuclei were stained with Topro (blue). Scale bar represents 100 µm. Panel C: Graphs of BrdU incorporation ±SEM illustrating the rates of proliferation of stroma and epithelia. Addition of hPTN led to an increase in stromal and epithelial cell proliferation only in the absence of testosterone (Student's t-test, **P < 0.01).
Mentions: To examine the effect of hPTN upon the cellular differentiation and proliferation of VPs grown in vitro (n = 3, 27 organs) we examined the organs by histology and calculated proliferative rates using immunohistochemistry for BrdU incorporation and stromal and epithelial markers (Fig. 4). Histology of the VP organs showed no substantial changes in organ morphology or stromal and epithelial distribution (Fig. 4A). Analysis of stromal and epithelial differentiation using SM α-actin and p63 showed no difference in cellular differentiation after treatment with hPTN, but confirmed increased branching of ductal tips at the periphery of the VP organs (Supplementary Fig. 2). The proliferative index of the epithelial and mesenchymal compartments in the peripheral region (distal to the urethra) was calculated for VPs cultured ±T, ±hPTN (Fig. 4B,C and values are listed in Supplementary Tables C and D). hPTN significantly increased epithelial (P < 0.05) and stromal (P < 0.01) proliferation in the absence of testosterone.

Bottom Line: Ptn transcripts and protein were localized by in situ and immunohistochemistry and Ptn mRNA was quantitated by Northern blot and qRT-PCR.PTN also showed male enriched expression in fetal human male urethra versus female, and between wt male and ARKO male mice.Ptn protein was increased by testosterone in organ cultures of female rat VMP and in rat male urethra compared to female.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, The Queen's Medical Research Institute, Edinburgh, UK.

Show MeSH
Related in: MedlinePlus