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Expression of pleiotrophin in the prostate is androgen regulated and it functions as an autocrine regulator of mesenchyme and cancer associated fibroblasts and as a paracrine regulator of epithelia.

Orr B, Vanpoucke G, Grace OC, Smith L, Anderson RA, Riddick AC, Franco OE, Hayward SW, Thomson AA - Prostate (2010)

Bottom Line: Ptn transcripts and protein were localized by in situ and immunohistochemistry and Ptn mRNA was quantitated by Northern blot and qRT-PCR.PTN also showed male enriched expression in fetal human male urethra versus female, and between wt male and ARKO male mice.Ptn protein was increased by testosterone in organ cultures of female rat VMP and in rat male urethra compared to female.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, The Queen's Medical Research Institute, Edinburgh, UK.

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The effect of recombinant hPTN on VPs grown in vitro. P0 VPs were cultured in the presence or absence of testosterone (10−8 M) and/or hPTN (3 µg/ml). Panel A: Whole-mount images of male VP organ cultures after 6 days; addition of hPTN led to small effects upon organ size and morphology. Scale bar represents 1 mm. Panel B: Graph showing the mean two-dimensional area of VPs relative to −T, ±SEM. Addition of hPTN led to a modest reduction in the size of organs grown in the presence of T, but not in organs grown in the absence of T. Panel C: Graph showing the mean number of epithelial bud tips around the periphery of cultured VPs, expressed as a ratio of organ perimeter (mean number of buds per 1,000 pixels perimeter ±SEM). Addition of hPTN led to a statistically significant (Student's t-test, **P < 0.01 and ***P < 0.001) increase in the number of epithelial bud tips/unit perimeter in VPs grown ±T.
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fig03: The effect of recombinant hPTN on VPs grown in vitro. P0 VPs were cultured in the presence or absence of testosterone (10−8 M) and/or hPTN (3 µg/ml). Panel A: Whole-mount images of male VP organ cultures after 6 days; addition of hPTN led to small effects upon organ size and morphology. Scale bar represents 1 mm. Panel B: Graph showing the mean two-dimensional area of VPs relative to −T, ±SEM. Addition of hPTN led to a modest reduction in the size of organs grown in the presence of T, but not in organs grown in the absence of T. Panel C: Graph showing the mean number of epithelial bud tips around the periphery of cultured VPs, expressed as a ratio of organ perimeter (mean number of buds per 1,000 pixels perimeter ±SEM). Addition of hPTN led to a statistically significant (Student's t-test, **P < 0.01 and ***P < 0.001) increase in the number of epithelial bud tips/unit perimeter in VPs grown ±T.

Mentions: The role of Ptn in prostate growth and morphogenesis was investigated using cultures of VP organs grown in vitro and treated with testosterone and recombinant hPTN protein (Fig. 3A). Addition of testosterone led to an increase in organ size and branching; however, addition of hPTN had relatively modest effects either in the presence or absence of testosterone (n = 5 experiments, 45 organs). The two dimensional area of VP organs grown ±T, ±3 µg/ml hPTN was measured using NIH imaging software (Fig. 3B, and Supplementary Table A). Addition of hPTN to VP organs had no statistically significant effect upon organ area, in the presence (P = 0.11) or absence (P = 0.4) of testosterone. hPTN (Peprotech) was added to organ cultures at physiological levels (∼0.94 µg/ml tissue volume) as used previously, to determine a functional response in vitro 39. To investigate a role for hPTN in branching morphogenesis, the number of epithelial bud tips around the periphery of VPs cultured ±T and ±hPTN were counted. The number of tips was expressed as a ratio to organ perimeter (tips per 1,000 pixels perimeter) to control for changes in organ size (Fig. 3C, and values are listed in Supplementary Table B). Treatment of VPs with hPTN caused an increase in the number of tips/1,000 pixels perimeter in the presence (P < 0.01) or absence (P < 0.001) of testosterone, and we suggest that Ptn has a role in increasing branching morphogenesis in the prostate.


Expression of pleiotrophin in the prostate is androgen regulated and it functions as an autocrine regulator of mesenchyme and cancer associated fibroblasts and as a paracrine regulator of epithelia.

Orr B, Vanpoucke G, Grace OC, Smith L, Anderson RA, Riddick AC, Franco OE, Hayward SW, Thomson AA - Prostate (2010)

The effect of recombinant hPTN on VPs grown in vitro. P0 VPs were cultured in the presence or absence of testosterone (10−8 M) and/or hPTN (3 µg/ml). Panel A: Whole-mount images of male VP organ cultures after 6 days; addition of hPTN led to small effects upon organ size and morphology. Scale bar represents 1 mm. Panel B: Graph showing the mean two-dimensional area of VPs relative to −T, ±SEM. Addition of hPTN led to a modest reduction in the size of organs grown in the presence of T, but not in organs grown in the absence of T. Panel C: Graph showing the mean number of epithelial bud tips around the periphery of cultured VPs, expressed as a ratio of organ perimeter (mean number of buds per 1,000 pixels perimeter ±SEM). Addition of hPTN led to a statistically significant (Student's t-test, **P < 0.01 and ***P < 0.001) increase in the number of epithelial bud tips/unit perimeter in VPs grown ±T.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3045659&req=5

fig03: The effect of recombinant hPTN on VPs grown in vitro. P0 VPs were cultured in the presence or absence of testosterone (10−8 M) and/or hPTN (3 µg/ml). Panel A: Whole-mount images of male VP organ cultures after 6 days; addition of hPTN led to small effects upon organ size and morphology. Scale bar represents 1 mm. Panel B: Graph showing the mean two-dimensional area of VPs relative to −T, ±SEM. Addition of hPTN led to a modest reduction in the size of organs grown in the presence of T, but not in organs grown in the absence of T. Panel C: Graph showing the mean number of epithelial bud tips around the periphery of cultured VPs, expressed as a ratio of organ perimeter (mean number of buds per 1,000 pixels perimeter ±SEM). Addition of hPTN led to a statistically significant (Student's t-test, **P < 0.01 and ***P < 0.001) increase in the number of epithelial bud tips/unit perimeter in VPs grown ±T.
Mentions: The role of Ptn in prostate growth and morphogenesis was investigated using cultures of VP organs grown in vitro and treated with testosterone and recombinant hPTN protein (Fig. 3A). Addition of testosterone led to an increase in organ size and branching; however, addition of hPTN had relatively modest effects either in the presence or absence of testosterone (n = 5 experiments, 45 organs). The two dimensional area of VP organs grown ±T, ±3 µg/ml hPTN was measured using NIH imaging software (Fig. 3B, and Supplementary Table A). Addition of hPTN to VP organs had no statistically significant effect upon organ area, in the presence (P = 0.11) or absence (P = 0.4) of testosterone. hPTN (Peprotech) was added to organ cultures at physiological levels (∼0.94 µg/ml tissue volume) as used previously, to determine a functional response in vitro 39. To investigate a role for hPTN in branching morphogenesis, the number of epithelial bud tips around the periphery of VPs cultured ±T and ±hPTN were counted. The number of tips was expressed as a ratio to organ perimeter (tips per 1,000 pixels perimeter) to control for changes in organ size (Fig. 3C, and values are listed in Supplementary Table B). Treatment of VPs with hPTN caused an increase in the number of tips/1,000 pixels perimeter in the presence (P < 0.01) or absence (P < 0.001) of testosterone, and we suggest that Ptn has a role in increasing branching morphogenesis in the prostate.

Bottom Line: Ptn transcripts and protein were localized by in situ and immunohistochemistry and Ptn mRNA was quantitated by Northern blot and qRT-PCR.PTN also showed male enriched expression in fetal human male urethra versus female, and between wt male and ARKO male mice.Ptn protein was increased by testosterone in organ cultures of female rat VMP and in rat male urethra compared to female.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, The Queen's Medical Research Institute, Edinburgh, UK.

Show MeSH
Related in: MedlinePlus