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Stimuli-responsive controlled-release system using quadruplex DNA-capped silica nanocontainers.

Chen C, Pu F, Huang Z, Liu Z, Ren J, Qu X - Nucleic Acids Res. (2010)

Bottom Line: Start from simple conformation changes, the i-motif DNA cap can open and close the pore system in smart response to pH stimulus.A pH-switchable nanoreactor has also been developed to validate the potential of our system for on-demand molecular transport.This proof of concept might open the door to a new generation of carrier materials and could also provide a general route to use other functional nucleic acids/peptide nucleic acids as capping agents in the fields of versatile controlled delivery nanodevices.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Chemical Biology and State Key laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China.

ABSTRACT
A novel proton-fueled molecular gate-like delivery system has been constructed for controlled cargo release using i-motif quadruplex DNA as caps onto pore outlets of mesoporous silica nanoparticles. Start from simple conformation changes, the i-motif DNA cap can open and close the pore system in smart response to pH stimulus. Importantly, the opening/closing and delivery protocol is highly reversible and a partial cargo delivery can be easily controlled at will. A pH-switchable nanoreactor has also been developed to validate the potential of our system for on-demand molecular transport. This proof of concept might open the door to a new generation of carrier materials and could also provide a general route to use other functional nucleic acids/peptide nucleic acids as capping agents in the fields of versatile controlled delivery nanodevices.

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(A) Nitrogen sorption isotherms of the samples (a) MSN-NH2, (b) MSN-DNA and (c) MSN-RhB. (B) FTIR spectra of the samples (a) MSN-NH2, (b) MSN-COOH and (c) MSN-DNA.
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Figure 2: (A) Nitrogen sorption isotherms of the samples (a) MSN-NH2, (b) MSN-DNA and (c) MSN-RhB. (B) FTIR spectra of the samples (a) MSN-NH2, (b) MSN-COOH and (c) MSN-DNA.

Mentions: The MCM-41 particle was synthesized using a base-catalyzed sol-gel procedure (23) and MSN (400 nm in diameter) that contain hexagonally arranged pores were confirmed by SEM and X-ray diffraction (Supplementary Figures S1 and S2). The surface of MSN was then functionalized with amine groups by treatment with 3-aminopropyltriethoxysilane (APTES) to afford MSN-NH2. N2 adsorption–desorption isotherms of MSN-NH2 showed a typical Type IV curve with a specific surface area of 1476 m2 g−1, average pore diameter of 2.14 nm and a narrow pore distribution (Figure 2A and Table 1). The silica particle functionalized with a carboxylic group (MSN-COOH) was obtained by allowing MSN-NH2 to react with succinic anhydride in N,N-dimetylformamide (DMF). The resultant carboxyl unit on the surface was activated by 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosucnimide sodium salt (sulfo-NHS) in a MES buffer and subsequently treated with DNA-NH2 to obtain MSN-DNA.Figure 2.


Stimuli-responsive controlled-release system using quadruplex DNA-capped silica nanocontainers.

Chen C, Pu F, Huang Z, Liu Z, Ren J, Qu X - Nucleic Acids Res. (2010)

(A) Nitrogen sorption isotherms of the samples (a) MSN-NH2, (b) MSN-DNA and (c) MSN-RhB. (B) FTIR spectra of the samples (a) MSN-NH2, (b) MSN-COOH and (c) MSN-DNA.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3045591&req=5

Figure 2: (A) Nitrogen sorption isotherms of the samples (a) MSN-NH2, (b) MSN-DNA and (c) MSN-RhB. (B) FTIR spectra of the samples (a) MSN-NH2, (b) MSN-COOH and (c) MSN-DNA.
Mentions: The MCM-41 particle was synthesized using a base-catalyzed sol-gel procedure (23) and MSN (400 nm in diameter) that contain hexagonally arranged pores were confirmed by SEM and X-ray diffraction (Supplementary Figures S1 and S2). The surface of MSN was then functionalized with amine groups by treatment with 3-aminopropyltriethoxysilane (APTES) to afford MSN-NH2. N2 adsorption–desorption isotherms of MSN-NH2 showed a typical Type IV curve with a specific surface area of 1476 m2 g−1, average pore diameter of 2.14 nm and a narrow pore distribution (Figure 2A and Table 1). The silica particle functionalized with a carboxylic group (MSN-COOH) was obtained by allowing MSN-NH2 to react with succinic anhydride in N,N-dimetylformamide (DMF). The resultant carboxyl unit on the surface was activated by 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosucnimide sodium salt (sulfo-NHS) in a MES buffer and subsequently treated with DNA-NH2 to obtain MSN-DNA.Figure 2.

Bottom Line: Start from simple conformation changes, the i-motif DNA cap can open and close the pore system in smart response to pH stimulus.A pH-switchable nanoreactor has also been developed to validate the potential of our system for on-demand molecular transport.This proof of concept might open the door to a new generation of carrier materials and could also provide a general route to use other functional nucleic acids/peptide nucleic acids as capping agents in the fields of versatile controlled delivery nanodevices.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Chemical Biology and State Key laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China.

ABSTRACT
A novel proton-fueled molecular gate-like delivery system has been constructed for controlled cargo release using i-motif quadruplex DNA as caps onto pore outlets of mesoporous silica nanoparticles. Start from simple conformation changes, the i-motif DNA cap can open and close the pore system in smart response to pH stimulus. Importantly, the opening/closing and delivery protocol is highly reversible and a partial cargo delivery can be easily controlled at will. A pH-switchable nanoreactor has also been developed to validate the potential of our system for on-demand molecular transport. This proof of concept might open the door to a new generation of carrier materials and could also provide a general route to use other functional nucleic acids/peptide nucleic acids as capping agents in the fields of versatile controlled delivery nanodevices.

Show MeSH
Related in: MedlinePlus