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Multiple invasions into freshwater by pufferfishes (teleostei: tetraodontidae): a mitogenomic perspective.

Yamanoue Y, Miya M, Doi H, Mabuchi K, Sakai H, Nishida M - PLoS ONE (2011)

Bottom Line: The resulting phylogenies reveal that the family is composed of four major lineages and that freshwater species from the different continents are independently nested in two of the four lineages.Relaxed molecular-clock Bayesian divergence time estimation suggests that the timing of these invasions differs between continents, occurring at 0-10 million years ago (MA) in South America, 17-38 MA in Central Africa, and 48-78 MA in Southeast Asia.These timings are congruent with geological events that could facilitate adaptation to freshwater habitats in each continent.

View Article: PubMed Central - PubMed

Affiliation: Atmosphere and Ocean Research Institute, University of Tokyo, Kashiwa, Chiba, Japan. yamanouey@yahoo.co.jp

ABSTRACT
Pufferfishes of the Family Tetraodontidae are the most speciose group in the Order Tetraodontiformes and mainly inhabit coastal waters along continents. Although no members of other tetraodontiform families have fully discarded their marine lives, approximately 30 tetraodontid species spend their entire lives in freshwaters in disjunct tropical regions of South America, Central Africa, and Southeast Asia. To investigate the interrelationships of tetraodontid pufferfishes and thereby elucidate the evolutionary origins of their freshwater habitats, we performed phylogenetic analysis based on whole mitochondrial genome sequences from 50 tetraodontid species and closely related species (including 31 newly determined sequences). The resulting phylogenies reveal that the family is composed of four major lineages and that freshwater species from the different continents are independently nested in two of the four lineages. A monophyletic origin of the use of freshwater habitats was statistically rejected, and ancestral habitat reconstruction on the resulting tree demonstrates that tetraodontids independently entered freshwater habitats in different continents at least three times. Relaxed molecular-clock Bayesian divergence time estimation suggests that the timing of these invasions differs between continents, occurring at 0-10 million years ago (MA) in South America, 17-38 MA in Central Africa, and 48-78 MA in Southeast Asia. These timings are congruent with geological events that could facilitate adaptation to freshwater habitats in each continent.

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Divergence times among tetraodontid pufferfishes from the relaxed-molecular clock method with the dataset 12nRTn.Node numbers 1–12 and three time constraints are indicated above and below nodes, respectively. Horizontal bars indicate 95% credible intervals of the divergence time estimates.
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pone-0017410-g004: Divergence times among tetraodontid pufferfishes from the relaxed-molecular clock method with the dataset 12nRTn.Node numbers 1–12 and three time constraints are indicated above and below nodes, respectively. Horizontal bars indicate 95% credible intervals of the divergence time estimates.

Mentions: MCMCTREE analysis of divergence times is shown in Figure 4 and Table 2 (Figure S1 and Table S1 for more information), with the 95% credible interval (CI). Tetraodontids are estimated to diverge from diodontids at 114 million years ago (MA) (89–138 MA), and the four major lineages of tetraodontid pufferfishes (Clades A–D) diverged from the ancestral lineage during the late Cretaceous (80–101 MA). The most recent common ancestors of Clades A–D date back to 41 MA (21–69 MA), 31 MA (21–45 MA), 50 MA (35–67 MA), and 78 MA (65–93 MA), respectively (Figure 4, Table 2). The South American freshwater species, Colomesus asellus, diverged from C. psittacus at 10 MA (4–18 MA) in Clade B (Figure 4, Table 2). Southeast Asian freshwater species diverged from the remaining members of Clade D at 78 MA (65–93 MA), and their most recent common ancestor emerged at 48 MA (32–66 MA) (Figure 4, Table 2). Central African freshwater species diverged from Chelondon species at 38 MA (21–59 MA), and their most recent common ancestor emerged at 17 MA (6–31 MA) (Figure 4, Table 2).


Multiple invasions into freshwater by pufferfishes (teleostei: tetraodontidae): a mitogenomic perspective.

Yamanoue Y, Miya M, Doi H, Mabuchi K, Sakai H, Nishida M - PLoS ONE (2011)

Divergence times among tetraodontid pufferfishes from the relaxed-molecular clock method with the dataset 12nRTn.Node numbers 1–12 and three time constraints are indicated above and below nodes, respectively. Horizontal bars indicate 95% credible intervals of the divergence time estimates.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3045446&req=5

pone-0017410-g004: Divergence times among tetraodontid pufferfishes from the relaxed-molecular clock method with the dataset 12nRTn.Node numbers 1–12 and three time constraints are indicated above and below nodes, respectively. Horizontal bars indicate 95% credible intervals of the divergence time estimates.
Mentions: MCMCTREE analysis of divergence times is shown in Figure 4 and Table 2 (Figure S1 and Table S1 for more information), with the 95% credible interval (CI). Tetraodontids are estimated to diverge from diodontids at 114 million years ago (MA) (89–138 MA), and the four major lineages of tetraodontid pufferfishes (Clades A–D) diverged from the ancestral lineage during the late Cretaceous (80–101 MA). The most recent common ancestors of Clades A–D date back to 41 MA (21–69 MA), 31 MA (21–45 MA), 50 MA (35–67 MA), and 78 MA (65–93 MA), respectively (Figure 4, Table 2). The South American freshwater species, Colomesus asellus, diverged from C. psittacus at 10 MA (4–18 MA) in Clade B (Figure 4, Table 2). Southeast Asian freshwater species diverged from the remaining members of Clade D at 78 MA (65–93 MA), and their most recent common ancestor emerged at 48 MA (32–66 MA) (Figure 4, Table 2). Central African freshwater species diverged from Chelondon species at 38 MA (21–59 MA), and their most recent common ancestor emerged at 17 MA (6–31 MA) (Figure 4, Table 2).

Bottom Line: The resulting phylogenies reveal that the family is composed of four major lineages and that freshwater species from the different continents are independently nested in two of the four lineages.Relaxed molecular-clock Bayesian divergence time estimation suggests that the timing of these invasions differs between continents, occurring at 0-10 million years ago (MA) in South America, 17-38 MA in Central Africa, and 48-78 MA in Southeast Asia.These timings are congruent with geological events that could facilitate adaptation to freshwater habitats in each continent.

View Article: PubMed Central - PubMed

Affiliation: Atmosphere and Ocean Research Institute, University of Tokyo, Kashiwa, Chiba, Japan. yamanouey@yahoo.co.jp

ABSTRACT
Pufferfishes of the Family Tetraodontidae are the most speciose group in the Order Tetraodontiformes and mainly inhabit coastal waters along continents. Although no members of other tetraodontiform families have fully discarded their marine lives, approximately 30 tetraodontid species spend their entire lives in freshwaters in disjunct tropical regions of South America, Central Africa, and Southeast Asia. To investigate the interrelationships of tetraodontid pufferfishes and thereby elucidate the evolutionary origins of their freshwater habitats, we performed phylogenetic analysis based on whole mitochondrial genome sequences from 50 tetraodontid species and closely related species (including 31 newly determined sequences). The resulting phylogenies reveal that the family is composed of four major lineages and that freshwater species from the different continents are independently nested in two of the four lineages. A monophyletic origin of the use of freshwater habitats was statistically rejected, and ancestral habitat reconstruction on the resulting tree demonstrates that tetraodontids independently entered freshwater habitats in different continents at least three times. Relaxed molecular-clock Bayesian divergence time estimation suggests that the timing of these invasions differs between continents, occurring at 0-10 million years ago (MA) in South America, 17-38 MA in Central Africa, and 48-78 MA in Southeast Asia. These timings are congruent with geological events that could facilitate adaptation to freshwater habitats in each continent.

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