Limits...
Multiple invasions into freshwater by pufferfishes (teleostei: tetraodontidae): a mitogenomic perspective.

Yamanoue Y, Miya M, Doi H, Mabuchi K, Sakai H, Nishida M - PLoS ONE (2011)

Bottom Line: The resulting phylogenies reveal that the family is composed of four major lineages and that freshwater species from the different continents are independently nested in two of the four lineages.Relaxed molecular-clock Bayesian divergence time estimation suggests that the timing of these invasions differs between continents, occurring at 0-10 million years ago (MA) in South America, 17-38 MA in Central Africa, and 48-78 MA in Southeast Asia.These timings are congruent with geological events that could facilitate adaptation to freshwater habitats in each continent.

View Article: PubMed Central - PubMed

Affiliation: Atmosphere and Ocean Research Institute, University of Tokyo, Kashiwa, Chiba, Japan. yamanouey@yahoo.co.jp

ABSTRACT
Pufferfishes of the Family Tetraodontidae are the most speciose group in the Order Tetraodontiformes and mainly inhabit coastal waters along continents. Although no members of other tetraodontiform families have fully discarded their marine lives, approximately 30 tetraodontid species spend their entire lives in freshwaters in disjunct tropical regions of South America, Central Africa, and Southeast Asia. To investigate the interrelationships of tetraodontid pufferfishes and thereby elucidate the evolutionary origins of their freshwater habitats, we performed phylogenetic analysis based on whole mitochondrial genome sequences from 50 tetraodontid species and closely related species (including 31 newly determined sequences). The resulting phylogenies reveal that the family is composed of four major lineages and that freshwater species from the different continents are independently nested in two of the four lineages. A monophyletic origin of the use of freshwater habitats was statistically rejected, and ancestral habitat reconstruction on the resulting tree demonstrates that tetraodontids independently entered freshwater habitats in different continents at least three times. Relaxed molecular-clock Bayesian divergence time estimation suggests that the timing of these invasions differs between continents, occurring at 0-10 million years ago (MA) in South America, 17-38 MA in Central Africa, and 48-78 MA in Southeast Asia. These timings are congruent with geological events that could facilitate adaptation to freshwater habitats in each continent.

Show MeSH
An ML tree with estimated branch lengths using the 12n3rRTn dataset.Topological differences between the ML analysis for the 12n3rRTn and 123nRTn datasets are indicated by arrowheads. Numbers near internal branches indicate ML bootstrap probabilities for the 12n3rRTn (left) and 123nRTn (right) datasets (values less than 50% not shown). Single numbers indicate that these estimates were identical.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3045446&req=5

pone-0017410-g003: An ML tree with estimated branch lengths using the 12n3rRTn dataset.Topological differences between the ML analysis for the 12n3rRTn and 123nRTn datasets are indicated by arrowheads. Numbers near internal branches indicate ML bootstrap probabilities for the 12n3rRTn (left) and 123nRTn (right) datasets (values less than 50% not shown). Single numbers indicate that these estimates were identical.

Mentions: Based on the patterns of sequence variation, the dataset with the third codon positions converted by RY-coding (12n3rRTn) was expected to effectively remove the noise expected from quickly saturated transitional changes in the third codon positions and avoid a lack of signal by retaining all available positions in the dataset [19], [20]. Accordingly, partitioned ML analysis based on the 12n3rRTn dataset is shown in Figure 3.


Multiple invasions into freshwater by pufferfishes (teleostei: tetraodontidae): a mitogenomic perspective.

Yamanoue Y, Miya M, Doi H, Mabuchi K, Sakai H, Nishida M - PLoS ONE (2011)

An ML tree with estimated branch lengths using the 12n3rRTn dataset.Topological differences between the ML analysis for the 12n3rRTn and 123nRTn datasets are indicated by arrowheads. Numbers near internal branches indicate ML bootstrap probabilities for the 12n3rRTn (left) and 123nRTn (right) datasets (values less than 50% not shown). Single numbers indicate that these estimates were identical.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3045446&req=5

pone-0017410-g003: An ML tree with estimated branch lengths using the 12n3rRTn dataset.Topological differences between the ML analysis for the 12n3rRTn and 123nRTn datasets are indicated by arrowheads. Numbers near internal branches indicate ML bootstrap probabilities for the 12n3rRTn (left) and 123nRTn (right) datasets (values less than 50% not shown). Single numbers indicate that these estimates were identical.
Mentions: Based on the patterns of sequence variation, the dataset with the third codon positions converted by RY-coding (12n3rRTn) was expected to effectively remove the noise expected from quickly saturated transitional changes in the third codon positions and avoid a lack of signal by retaining all available positions in the dataset [19], [20]. Accordingly, partitioned ML analysis based on the 12n3rRTn dataset is shown in Figure 3.

Bottom Line: The resulting phylogenies reveal that the family is composed of four major lineages and that freshwater species from the different continents are independently nested in two of the four lineages.Relaxed molecular-clock Bayesian divergence time estimation suggests that the timing of these invasions differs between continents, occurring at 0-10 million years ago (MA) in South America, 17-38 MA in Central Africa, and 48-78 MA in Southeast Asia.These timings are congruent with geological events that could facilitate adaptation to freshwater habitats in each continent.

View Article: PubMed Central - PubMed

Affiliation: Atmosphere and Ocean Research Institute, University of Tokyo, Kashiwa, Chiba, Japan. yamanouey@yahoo.co.jp

ABSTRACT
Pufferfishes of the Family Tetraodontidae are the most speciose group in the Order Tetraodontiformes and mainly inhabit coastal waters along continents. Although no members of other tetraodontiform families have fully discarded their marine lives, approximately 30 tetraodontid species spend their entire lives in freshwaters in disjunct tropical regions of South America, Central Africa, and Southeast Asia. To investigate the interrelationships of tetraodontid pufferfishes and thereby elucidate the evolutionary origins of their freshwater habitats, we performed phylogenetic analysis based on whole mitochondrial genome sequences from 50 tetraodontid species and closely related species (including 31 newly determined sequences). The resulting phylogenies reveal that the family is composed of four major lineages and that freshwater species from the different continents are independently nested in two of the four lineages. A monophyletic origin of the use of freshwater habitats was statistically rejected, and ancestral habitat reconstruction on the resulting tree demonstrates that tetraodontids independently entered freshwater habitats in different continents at least three times. Relaxed molecular-clock Bayesian divergence time estimation suggests that the timing of these invasions differs between continents, occurring at 0-10 million years ago (MA) in South America, 17-38 MA in Central Africa, and 48-78 MA in Southeast Asia. These timings are congruent with geological events that could facilitate adaptation to freshwater habitats in each continent.

Show MeSH