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Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L.

Sohn EH, Jang SA, Joo H, Park S, Kang SC, Lee CH, Kim SY - Clin Mol Allergy (2011)

Bottom Line: This study examined whether a butanol extract of A. lappa (ALBE) had previously unreported anti-allergic or anti-inflammatory effects.This study examined the effect of ALBE on the release of β-hexosaminidase in antigen-stimulated-RBL-2H3 cells.We observed significant inhibition of β-hexosaminidase release in RBL-2H3 cells and suppressed mRNA expression and protein secretion of IL-4 and IL-5 induced by ConA-treated primary murine splenocytes after ALBE treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pediatrics, College of Medicine, Hanyang University, Seoul, 133-792, Korea. nel1205@hanmail.net.

ABSTRACT

Background: Atopic dermatitis is a chronic, allergic inflammatory skin disease that is accompanied by markedly increased levels of inflammatory cells, including eosinophils, mast cells, and T cells. Arctium lappa L. is a traditional medicine in Asia. This study examined whether a butanol extract of A. lappa (ALBE) had previously unreported anti-allergic or anti-inflammatory effects.

Methods: This study examined the effect of ALBE on the release of β-hexosaminidase in antigen-stimulated-RBL-2H3 cells. We also evaluated the ConA-induced expression of IL-4, IL-5, mitogen-activated protein kinases (MAPKs), and nuclear factor (NF)-κB using RT-PCR, Western blotting, and ELISA in mouse splenocytes after ALBE treatment.

Results: We observed significant inhibition of β-hexosaminidase release in RBL-2H3 cells and suppressed mRNA expression and protein secretion of IL-4 and IL-5 induced by ConA-treated primary murine splenocytes after ALBE treatment. Additionally, ALBE (100 μg/mL) suppressed not only the transcriptional activation of NF-κB, but also the phosphorylation of MAPKs in ConA-treated primary splenocytes.

Conclusions: These results suggest that ALBE inhibits the expression of IL-4 and IL-5 by downregulating MAPKs and NF-κB activation in ConA-treated splenocytes and supports the hypothesis that ALBE may have beneficial effects in the treatment of allergic diseases, including atopic dermatitis.

No MeSH data available.


Related in: MedlinePlus

Effects of ALBE on phosphorylations of p38 MAPK in ConA-induced primary murine splenocytes. ALBE (100 μg/ml) were treated to splenocytes with or without ConA (3 μg/ml) for 15 min. The phosphorylations of p38 MAP kinase such as p38, JNK and ERK were assessed by Western blotting described in methods. #P < 0.05, ##P < 0.01: significantly different from the untreated group. **P < 0.01: significantly different from the ConA alone group.
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Figure 6: Effects of ALBE on phosphorylations of p38 MAPK in ConA-induced primary murine splenocytes. ALBE (100 μg/ml) were treated to splenocytes with or without ConA (3 μg/ml) for 15 min. The phosphorylations of p38 MAP kinase such as p38, JNK and ERK were assessed by Western blotting described in methods. #P < 0.05, ##P < 0.01: significantly different from the untreated group. **P < 0.01: significantly different from the ConA alone group.

Mentions: Increased expression of NF-κB (p65) was observed in the nucleus after treatment with ALBE plus ConA for 4 h (Figure 5). The relative intensity of NF-κB (p65) translocation in the nucleus was increased to 6.3% in the presence of ConA compared with the absence of ConA in the control. In contrast, the relative intensity of NF-κB (p65) translocation in the nucleus was decreased considerably, to 8.7%, after the addition of 100 μg/mL ALBE in the presence of ConA compared with ConA treatment alone. These data demonstrate that ALBE attenuated NF-κB activation and might affect downstream IL-4 and IL-5 production. ALBE inhibits ConA-induced phosphorylation of MAP kinases such as p38, JNK, and ERK (Figure 6). We found that ALBE attenuated not only the ConA-induced increase in the activity of NF-κB, but also the phosphorylation of MAPKs and these results suggest that ALBE may prevent allergic and atopic inflammation via NF-κB and the MAPKs signaling pathway.


Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L.

Sohn EH, Jang SA, Joo H, Park S, Kang SC, Lee CH, Kim SY - Clin Mol Allergy (2011)

Effects of ALBE on phosphorylations of p38 MAPK in ConA-induced primary murine splenocytes. ALBE (100 μg/ml) were treated to splenocytes with or without ConA (3 μg/ml) for 15 min. The phosphorylations of p38 MAP kinase such as p38, JNK and ERK were assessed by Western blotting described in methods. #P < 0.05, ##P < 0.01: significantly different from the untreated group. **P < 0.01: significantly different from the ConA alone group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3045362&req=5

Figure 6: Effects of ALBE on phosphorylations of p38 MAPK in ConA-induced primary murine splenocytes. ALBE (100 μg/ml) were treated to splenocytes with or without ConA (3 μg/ml) for 15 min. The phosphorylations of p38 MAP kinase such as p38, JNK and ERK were assessed by Western blotting described in methods. #P < 0.05, ##P < 0.01: significantly different from the untreated group. **P < 0.01: significantly different from the ConA alone group.
Mentions: Increased expression of NF-κB (p65) was observed in the nucleus after treatment with ALBE plus ConA for 4 h (Figure 5). The relative intensity of NF-κB (p65) translocation in the nucleus was increased to 6.3% in the presence of ConA compared with the absence of ConA in the control. In contrast, the relative intensity of NF-κB (p65) translocation in the nucleus was decreased considerably, to 8.7%, after the addition of 100 μg/mL ALBE in the presence of ConA compared with ConA treatment alone. These data demonstrate that ALBE attenuated NF-κB activation and might affect downstream IL-4 and IL-5 production. ALBE inhibits ConA-induced phosphorylation of MAP kinases such as p38, JNK, and ERK (Figure 6). We found that ALBE attenuated not only the ConA-induced increase in the activity of NF-κB, but also the phosphorylation of MAPKs and these results suggest that ALBE may prevent allergic and atopic inflammation via NF-κB and the MAPKs signaling pathway.

Bottom Line: This study examined whether a butanol extract of A. lappa (ALBE) had previously unreported anti-allergic or anti-inflammatory effects.This study examined the effect of ALBE on the release of β-hexosaminidase in antigen-stimulated-RBL-2H3 cells.We observed significant inhibition of β-hexosaminidase release in RBL-2H3 cells and suppressed mRNA expression and protein secretion of IL-4 and IL-5 induced by ConA-treated primary murine splenocytes after ALBE treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pediatrics, College of Medicine, Hanyang University, Seoul, 133-792, Korea. nel1205@hanmail.net.

ABSTRACT

Background: Atopic dermatitis is a chronic, allergic inflammatory skin disease that is accompanied by markedly increased levels of inflammatory cells, including eosinophils, mast cells, and T cells. Arctium lappa L. is a traditional medicine in Asia. This study examined whether a butanol extract of A. lappa (ALBE) had previously unreported anti-allergic or anti-inflammatory effects.

Methods: This study examined the effect of ALBE on the release of β-hexosaminidase in antigen-stimulated-RBL-2H3 cells. We also evaluated the ConA-induced expression of IL-4, IL-5, mitogen-activated protein kinases (MAPKs), and nuclear factor (NF)-κB using RT-PCR, Western blotting, and ELISA in mouse splenocytes after ALBE treatment.

Results: We observed significant inhibition of β-hexosaminidase release in RBL-2H3 cells and suppressed mRNA expression and protein secretion of IL-4 and IL-5 induced by ConA-treated primary murine splenocytes after ALBE treatment. Additionally, ALBE (100 μg/mL) suppressed not only the transcriptional activation of NF-κB, but also the phosphorylation of MAPKs in ConA-treated primary splenocytes.

Conclusions: These results suggest that ALBE inhibits the expression of IL-4 and IL-5 by downregulating MAPKs and NF-κB activation in ConA-treated splenocytes and supports the hypothesis that ALBE may have beneficial effects in the treatment of allergic diseases, including atopic dermatitis.

No MeSH data available.


Related in: MedlinePlus