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Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L.

Sohn EH, Jang SA, Joo H, Park S, Kang SC, Lee CH, Kim SY - Clin Mol Allergy (2011)

Bottom Line: This study examined whether a butanol extract of A. lappa (ALBE) had previously unreported anti-allergic or anti-inflammatory effects.This study examined the effect of ALBE on the release of β-hexosaminidase in antigen-stimulated-RBL-2H3 cells.We observed significant inhibition of β-hexosaminidase release in RBL-2H3 cells and suppressed mRNA expression and protein secretion of IL-4 and IL-5 induced by ConA-treated primary murine splenocytes after ALBE treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pediatrics, College of Medicine, Hanyang University, Seoul, 133-792, Korea. nel1205@hanmail.net.

ABSTRACT

Background: Atopic dermatitis is a chronic, allergic inflammatory skin disease that is accompanied by markedly increased levels of inflammatory cells, including eosinophils, mast cells, and T cells. Arctium lappa L. is a traditional medicine in Asia. This study examined whether a butanol extract of A. lappa (ALBE) had previously unreported anti-allergic or anti-inflammatory effects.

Methods: This study examined the effect of ALBE on the release of β-hexosaminidase in antigen-stimulated-RBL-2H3 cells. We also evaluated the ConA-induced expression of IL-4, IL-5, mitogen-activated protein kinases (MAPKs), and nuclear factor (NF)-κB using RT-PCR, Western blotting, and ELISA in mouse splenocytes after ALBE treatment.

Results: We observed significant inhibition of β-hexosaminidase release in RBL-2H3 cells and suppressed mRNA expression and protein secretion of IL-4 and IL-5 induced by ConA-treated primary murine splenocytes after ALBE treatment. Additionally, ALBE (100 μg/mL) suppressed not only the transcriptional activation of NF-κB, but also the phosphorylation of MAPKs in ConA-treated primary splenocytes.

Conclusions: These results suggest that ALBE inhibits the expression of IL-4 and IL-5 by downregulating MAPKs and NF-κB activation in ConA-treated splenocytes and supports the hypothesis that ALBE may have beneficial effects in the treatment of allergic diseases, including atopic dermatitis.

No MeSH data available.


Related in: MedlinePlus

Effects of ALBE on the proliferation of ConA-induced primary murine splenocytes. Splenocytes were treated with various concentrations of ALBE and ConA (3 μg/ml) for 72 h. Cell proliferation was assessed using XTT assays. Absorbance was measure data at 450 nm and 650 nm. Each bar shows the means ± SEM of four independent experiments. ##P < 0.01: significantly different from the untreated group. **P < 0.01: significantly different from the ConA alone group.
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Figure 2: Effects of ALBE on the proliferation of ConA-induced primary murine splenocytes. Splenocytes were treated with various concentrations of ALBE and ConA (3 μg/ml) for 72 h. Cell proliferation was assessed using XTT assays. Absorbance was measure data at 450 nm and 650 nm. Each bar shows the means ± SEM of four independent experiments. ##P < 0.01: significantly different from the untreated group. **P < 0.01: significantly different from the ConA alone group.

Mentions: We examined the effects of ALBE on ConA-induced T cell proliferation in primary murine splenocytes for 72 h to examine the immunomodulatory effect of ALBE. The concentration and duration of ALBE treatment without ConA had no effect on splenocyte viability (data not shown). As shown in Figure 2, ALBE significantly increased splenocyte proliferation in ConA-treated cells at 10 and 100 μg/mL (p < 0.05). Additionally, we examined the effects of ALBE on the expression and secretion of Th2 cytokines, such as IL-4 and IL-5, in primary murine splenocytes using RT-PCR and ELISA assays to investigate the further involvement of ALBE in Th2 functions in the atopic dermatitis-like skin lesions. ConA-induced IL-4 and IL-5 secretion was suppressed by ALBE treatment in splenocytes (Figure 3, Figure 4). ALBE treatment without ConA had no effect on IL-4 or IL-5 mRNA expression (data not shown), whereas ALBE with ConA significantly decreased the mRNA expression of IL-4 (to 55.3%) and IL-5 (to 29.0%) at 100 μg/mL, compared with ConA-stimulated splenocytes (Figure 3A, Figure 4A). In agreement with the RT-PCR results, ALBE inhibited the protein secretion of IL-4 (to 13.6%) and IL-5 (to 10.8%) under the same conditions (Figure 3B, Figure 4B). These results suggest that ALBE had immunostimulatory effects on T cells and meaningfully inhibited the antigen-induced mRNA expression and production of cytokines related to allergic and atopic reactions.


Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L.

Sohn EH, Jang SA, Joo H, Park S, Kang SC, Lee CH, Kim SY - Clin Mol Allergy (2011)

Effects of ALBE on the proliferation of ConA-induced primary murine splenocytes. Splenocytes were treated with various concentrations of ALBE and ConA (3 μg/ml) for 72 h. Cell proliferation was assessed using XTT assays. Absorbance was measure data at 450 nm and 650 nm. Each bar shows the means ± SEM of four independent experiments. ##P < 0.01: significantly different from the untreated group. **P < 0.01: significantly different from the ConA alone group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3045362&req=5

Figure 2: Effects of ALBE on the proliferation of ConA-induced primary murine splenocytes. Splenocytes were treated with various concentrations of ALBE and ConA (3 μg/ml) for 72 h. Cell proliferation was assessed using XTT assays. Absorbance was measure data at 450 nm and 650 nm. Each bar shows the means ± SEM of four independent experiments. ##P < 0.01: significantly different from the untreated group. **P < 0.01: significantly different from the ConA alone group.
Mentions: We examined the effects of ALBE on ConA-induced T cell proliferation in primary murine splenocytes for 72 h to examine the immunomodulatory effect of ALBE. The concentration and duration of ALBE treatment without ConA had no effect on splenocyte viability (data not shown). As shown in Figure 2, ALBE significantly increased splenocyte proliferation in ConA-treated cells at 10 and 100 μg/mL (p < 0.05). Additionally, we examined the effects of ALBE on the expression and secretion of Th2 cytokines, such as IL-4 and IL-5, in primary murine splenocytes using RT-PCR and ELISA assays to investigate the further involvement of ALBE in Th2 functions in the atopic dermatitis-like skin lesions. ConA-induced IL-4 and IL-5 secretion was suppressed by ALBE treatment in splenocytes (Figure 3, Figure 4). ALBE treatment without ConA had no effect on IL-4 or IL-5 mRNA expression (data not shown), whereas ALBE with ConA significantly decreased the mRNA expression of IL-4 (to 55.3%) and IL-5 (to 29.0%) at 100 μg/mL, compared with ConA-stimulated splenocytes (Figure 3A, Figure 4A). In agreement with the RT-PCR results, ALBE inhibited the protein secretion of IL-4 (to 13.6%) and IL-5 (to 10.8%) under the same conditions (Figure 3B, Figure 4B). These results suggest that ALBE had immunostimulatory effects on T cells and meaningfully inhibited the antigen-induced mRNA expression and production of cytokines related to allergic and atopic reactions.

Bottom Line: This study examined whether a butanol extract of A. lappa (ALBE) had previously unreported anti-allergic or anti-inflammatory effects.This study examined the effect of ALBE on the release of β-hexosaminidase in antigen-stimulated-RBL-2H3 cells.We observed significant inhibition of β-hexosaminidase release in RBL-2H3 cells and suppressed mRNA expression and protein secretion of IL-4 and IL-5 induced by ConA-treated primary murine splenocytes after ALBE treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pediatrics, College of Medicine, Hanyang University, Seoul, 133-792, Korea. nel1205@hanmail.net.

ABSTRACT

Background: Atopic dermatitis is a chronic, allergic inflammatory skin disease that is accompanied by markedly increased levels of inflammatory cells, including eosinophils, mast cells, and T cells. Arctium lappa L. is a traditional medicine in Asia. This study examined whether a butanol extract of A. lappa (ALBE) had previously unreported anti-allergic or anti-inflammatory effects.

Methods: This study examined the effect of ALBE on the release of β-hexosaminidase in antigen-stimulated-RBL-2H3 cells. We also evaluated the ConA-induced expression of IL-4, IL-5, mitogen-activated protein kinases (MAPKs), and nuclear factor (NF)-κB using RT-PCR, Western blotting, and ELISA in mouse splenocytes after ALBE treatment.

Results: We observed significant inhibition of β-hexosaminidase release in RBL-2H3 cells and suppressed mRNA expression and protein secretion of IL-4 and IL-5 induced by ConA-treated primary murine splenocytes after ALBE treatment. Additionally, ALBE (100 μg/mL) suppressed not only the transcriptional activation of NF-κB, but also the phosphorylation of MAPKs in ConA-treated primary splenocytes.

Conclusions: These results suggest that ALBE inhibits the expression of IL-4 and IL-5 by downregulating MAPKs and NF-κB activation in ConA-treated splenocytes and supports the hypothesis that ALBE may have beneficial effects in the treatment of allergic diseases, including atopic dermatitis.

No MeSH data available.


Related in: MedlinePlus