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Application of spectroscopic techniques: ICP-OES, LA-ICP-MS and chemometric methods for studying the relationships between trace elements in clinical samples from patients with atherosclerosis obliterans.

Hanć A, Komorowicz I, Iskra M, Majewski W, Barałkiewicz D - Anal Bioanal Chem (2011)

Bottom Line: Elements are not equally distributed between the inner (intima) and the outer (media + adventitia) layer of the arterial wall.Increased concentration of calcium found in the inner part of the atherosclerotic arterial wall and in the plaque, as compared to the control arterial wall samples, demonstrates the unquestionable role of this element in the calcification of the wall observed in AO.Applied chemometric methods were useful for demonstrating the differences in the element's concentration in blood serum and the arterial wall samples between AO and the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Trace Element Analysis by Spectroscopy Methods, Faculty of Chemistry, Adam Mickiewicz University, 6 Grunwaldzka Street, 60-780 Poznań, Poland.

ABSTRACT
The study was aimed to evaluate the influence of the vascular disease, atherosclerotic obliterans (AO), on the location and concentration of elements in the arterial wall and serum. Use of a modern method for studying element's concentration and distribution in samples of clinical material, i.e. laser ablation inductively coupled plasma mass spectrometry, is presented. Elements are not equally distributed between the inner (intima) and the outer (media + adventitia) layer of the arterial wall. Among the studied elements, calcium was found to have an unquestionable role in the calcification of the wall. Increased concentration of calcium found in the inner part of the atherosclerotic arterial wall and in the plaque, as compared to the control arterial wall samples, demonstrates the unquestionable role of this element in the calcification of the wall observed in AO. Applied chemometric methods were useful for demonstrating the differences in the element's concentration in blood serum and the arterial wall samples between AO and the control group.

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Pulse frequency level dependence on the 13C ion signal. Values are the mean of five measurements ± standard deviations
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Fig2: Pulse frequency level dependence on the 13C ion signal. Values are the mean of five measurements ± standard deviations

Mentions: The sample behaviour under the influence of laser beam differs depending on the type of material which is being exposed to laser ablation. Therefore, a very important step is the selection of laser ablation certain parameters such as scanning method, scanning rate, laser energy level, repetition rate and spot diameter [17, 18]. The optimization of the laser parameters was carried out using a single variable method. The energy of laser performance was investigated in the range of 2.25–9 mJ, at the same time registering the intensity of the analytical signal of 13C as an internal standard (Fig. 1). Measurements were carried out for the spot sizes of 25, 50 and 100 μm. Laser ablation of the sample using 7.2 mJ proved to be the most optimal, and the precision of measurement calculated as %RSD was 4%. The pulse frequency was compared in the range of 1–20 Hz; the results of the dependence of the intensity of the analytical signal of carbon isotope on the chosen pulse frequency were shown in Fig. 2. The precision of measurement was also compared, indicated as %RSD, depending on the pulse frequency used. After evaluation, the single-line scan method was used. Additionally, the effect of the scan rate on ion signals was studied. The sample size limited to a scan rate of 25 μm s−1 was selected in this study.Fig. 1


Application of spectroscopic techniques: ICP-OES, LA-ICP-MS and chemometric methods for studying the relationships between trace elements in clinical samples from patients with atherosclerosis obliterans.

Hanć A, Komorowicz I, Iskra M, Majewski W, Barałkiewicz D - Anal Bioanal Chem (2011)

Pulse frequency level dependence on the 13C ion signal. Values are the mean of five measurements ± standard deviations
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3044238&req=5

Fig2: Pulse frequency level dependence on the 13C ion signal. Values are the mean of five measurements ± standard deviations
Mentions: The sample behaviour under the influence of laser beam differs depending on the type of material which is being exposed to laser ablation. Therefore, a very important step is the selection of laser ablation certain parameters such as scanning method, scanning rate, laser energy level, repetition rate and spot diameter [17, 18]. The optimization of the laser parameters was carried out using a single variable method. The energy of laser performance was investigated in the range of 2.25–9 mJ, at the same time registering the intensity of the analytical signal of 13C as an internal standard (Fig. 1). Measurements were carried out for the spot sizes of 25, 50 and 100 μm. Laser ablation of the sample using 7.2 mJ proved to be the most optimal, and the precision of measurement calculated as %RSD was 4%. The pulse frequency was compared in the range of 1–20 Hz; the results of the dependence of the intensity of the analytical signal of carbon isotope on the chosen pulse frequency were shown in Fig. 2. The precision of measurement was also compared, indicated as %RSD, depending on the pulse frequency used. After evaluation, the single-line scan method was used. Additionally, the effect of the scan rate on ion signals was studied. The sample size limited to a scan rate of 25 μm s−1 was selected in this study.Fig. 1

Bottom Line: Elements are not equally distributed between the inner (intima) and the outer (media + adventitia) layer of the arterial wall.Increased concentration of calcium found in the inner part of the atherosclerotic arterial wall and in the plaque, as compared to the control arterial wall samples, demonstrates the unquestionable role of this element in the calcification of the wall observed in AO.Applied chemometric methods were useful for demonstrating the differences in the element's concentration in blood serum and the arterial wall samples between AO and the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Trace Element Analysis by Spectroscopy Methods, Faculty of Chemistry, Adam Mickiewicz University, 6 Grunwaldzka Street, 60-780 Poznań, Poland.

ABSTRACT
The study was aimed to evaluate the influence of the vascular disease, atherosclerotic obliterans (AO), on the location and concentration of elements in the arterial wall and serum. Use of a modern method for studying element's concentration and distribution in samples of clinical material, i.e. laser ablation inductively coupled plasma mass spectrometry, is presented. Elements are not equally distributed between the inner (intima) and the outer (media + adventitia) layer of the arterial wall. Among the studied elements, calcium was found to have an unquestionable role in the calcification of the wall. Increased concentration of calcium found in the inner part of the atherosclerotic arterial wall and in the plaque, as compared to the control arterial wall samples, demonstrates the unquestionable role of this element in the calcification of the wall observed in AO. Applied chemometric methods were useful for demonstrating the differences in the element's concentration in blood serum and the arterial wall samples between AO and the control group.

Show MeSH
Related in: MedlinePlus