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Fabrication of silver nanoparticles doped in the zeolite framework and antibacterial activity.

Shameli K, Ahmad MB, Zargar M, Yunus WM, Ibrahim NA - Int J Nanomedicine (2011)

Bottom Line: The results show that Ag NPs form a spherical shape with uniform homogeneity in the particle size.All of the synthesized Ag/zeolite NCs were found to have antibacterial activity.These results show that Ag NPs in the zeolite framework can be useful in different biological research and biomedical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia. kamyarshameli@gmail.com

ABSTRACT
Using the chemical reduction method, silver nanoparticles (Ag NPs) were effectively synthesized into the zeolite framework in the absence of any heat treatment. Zeolite, silver nitrate, and sodium borohydride were used as an inorganic solid support, a silver precursor, and a chemical reduction agent, respectively. Silver ions were introduced into the porous zeolite lattice by an ion-exchange path. After the reduction process, Ag NPs formed in the zeolite framework, with a mean diameter of about 2.12-3.11 nm. The most favorable experimental condition for the synthesis of Ag/zeolite nanocomposites (NCs) is described in terms of the initial concentration of AgNO(3). The Ag/zeolite NCs were characterized by ultraviolet-visible spectroscopy, powder X-ray diffraction, transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray fluorescence, and Fourier transform infrared. The results show that Ag NPs form a spherical shape with uniform homogeneity in the particle size. The antibacterial activity of Ag NPs in zeolites was investigated against Gram-negative bacteria (ie, Escherichia coli and Shigella dysentriae) and Gram-positive bacteria (ie, Staphylococcus aureus and methicillin-resistant Staphylococcus aureus) by disk diffusion method using Mueller-Hinton agar at different sizes of Ag NPs. All of the synthesized Ag/zeolite NCs were found to have antibacterial activity. These results show that Ag NPs in the zeolite framework can be useful in different biological research and biomedical applications.

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Scanning electron microscopy micrographs and energy dispersive X-ray fluorescence spectrometer spectra, respectively, for the zeolite (A, B) and silver/zeolite nanocomposites (A2 1.0% [C, D], A4 2.0% [E, F], and A5 5.0% [G, H]).
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f7-ijn-6-331: Scanning electron microscopy micrographs and energy dispersive X-ray fluorescence spectrometer spectra, respectively, for the zeolite (A, B) and silver/zeolite nanocomposites (A2 1.0% [C, D], A4 2.0% [E, F], and A5 5.0% [G, H]).

Mentions: The schematic illustration of the synthesis of Ag/zeolite NCs from AgNO3/zeolite is depicted in Figure 1. Meanwhile, as shown in Figure 2, the AgNO3/zeolite suspensions (A0) were colorless; after the addition of the reducing agent, however, they turned to light brown (A1 and A2), brown (A3), and dark brown (A4 and A5), indicating the formation of Ag NPs in the zeolite framework. The formation of Ag NPs was followed by measuring the surface plasmon resonance (SPR) band peaks of the AgNO3/zeolite and Ag/zeolite NCs at the wavelength ranging from 300 nm to 700 nm (Figure 3). The PXRD patterns of zeolite and Ag/zeolite NCs (A1–A5) in the wide angle range of 2θ (5°<2θ< 80°) were compared in order to determine the crystalline structures of the synthesized Ag NPs (Figure 4). The TEM images of zeolite and AgNO3/zeolite do not show any particle size of the nanosilver; however, in the Ag/zeolite NCs (A2, A4, and A5), the mean diameter of the NPs ranged from about 2.12 nm to 3.11 nm (Figures 5 and 6). As shown in Figure 7, the SEM images indicated that there were no structural changes in the initial zeolite and Ag/zeolite NCs (A2, A4, and A5) at different AgNO3 concentrations. Additionally, the EDXRF spectra for the zeolite and Ag/zeolite NCs (A2, A4, and A5) confirmed the presence of elemental compounds in the zeolite and Ag NPs without any other impurity peaks. The chemical structures of zeolite and Ag/zeolite NCs (A2, A4, and A5) were analyzed using FT-IR spectroscopy (Figure 8). The approximate efficiency gradually increased from A1 to A5 (Table 1). The antibacterial studies showed comparatively similar effects for all samples, as indicated by the inhibition zone test between zeolite, AgNO3/zeolite, and Ag/zeolite NCs (A2, A4, and A5) against different bacteria (Figure 9, Table 2).


Fabrication of silver nanoparticles doped in the zeolite framework and antibacterial activity.

Shameli K, Ahmad MB, Zargar M, Yunus WM, Ibrahim NA - Int J Nanomedicine (2011)

Scanning electron microscopy micrographs and energy dispersive X-ray fluorescence spectrometer spectra, respectively, for the zeolite (A, B) and silver/zeolite nanocomposites (A2 1.0% [C, D], A4 2.0% [E, F], and A5 5.0% [G, H]).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3044186&req=5

f7-ijn-6-331: Scanning electron microscopy micrographs and energy dispersive X-ray fluorescence spectrometer spectra, respectively, for the zeolite (A, B) and silver/zeolite nanocomposites (A2 1.0% [C, D], A4 2.0% [E, F], and A5 5.0% [G, H]).
Mentions: The schematic illustration of the synthesis of Ag/zeolite NCs from AgNO3/zeolite is depicted in Figure 1. Meanwhile, as shown in Figure 2, the AgNO3/zeolite suspensions (A0) were colorless; after the addition of the reducing agent, however, they turned to light brown (A1 and A2), brown (A3), and dark brown (A4 and A5), indicating the formation of Ag NPs in the zeolite framework. The formation of Ag NPs was followed by measuring the surface plasmon resonance (SPR) band peaks of the AgNO3/zeolite and Ag/zeolite NCs at the wavelength ranging from 300 nm to 700 nm (Figure 3). The PXRD patterns of zeolite and Ag/zeolite NCs (A1–A5) in the wide angle range of 2θ (5°<2θ< 80°) were compared in order to determine the crystalline structures of the synthesized Ag NPs (Figure 4). The TEM images of zeolite and AgNO3/zeolite do not show any particle size of the nanosilver; however, in the Ag/zeolite NCs (A2, A4, and A5), the mean diameter of the NPs ranged from about 2.12 nm to 3.11 nm (Figures 5 and 6). As shown in Figure 7, the SEM images indicated that there were no structural changes in the initial zeolite and Ag/zeolite NCs (A2, A4, and A5) at different AgNO3 concentrations. Additionally, the EDXRF spectra for the zeolite and Ag/zeolite NCs (A2, A4, and A5) confirmed the presence of elemental compounds in the zeolite and Ag NPs without any other impurity peaks. The chemical structures of zeolite and Ag/zeolite NCs (A2, A4, and A5) were analyzed using FT-IR spectroscopy (Figure 8). The approximate efficiency gradually increased from A1 to A5 (Table 1). The antibacterial studies showed comparatively similar effects for all samples, as indicated by the inhibition zone test between zeolite, AgNO3/zeolite, and Ag/zeolite NCs (A2, A4, and A5) against different bacteria (Figure 9, Table 2).

Bottom Line: The results show that Ag NPs form a spherical shape with uniform homogeneity in the particle size.All of the synthesized Ag/zeolite NCs were found to have antibacterial activity.These results show that Ag NPs in the zeolite framework can be useful in different biological research and biomedical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia. kamyarshameli@gmail.com

ABSTRACT
Using the chemical reduction method, silver nanoparticles (Ag NPs) were effectively synthesized into the zeolite framework in the absence of any heat treatment. Zeolite, silver nitrate, and sodium borohydride were used as an inorganic solid support, a silver precursor, and a chemical reduction agent, respectively. Silver ions were introduced into the porous zeolite lattice by an ion-exchange path. After the reduction process, Ag NPs formed in the zeolite framework, with a mean diameter of about 2.12-3.11 nm. The most favorable experimental condition for the synthesis of Ag/zeolite nanocomposites (NCs) is described in terms of the initial concentration of AgNO(3). The Ag/zeolite NCs were characterized by ultraviolet-visible spectroscopy, powder X-ray diffraction, transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray fluorescence, and Fourier transform infrared. The results show that Ag NPs form a spherical shape with uniform homogeneity in the particle size. The antibacterial activity of Ag NPs in zeolites was investigated against Gram-negative bacteria (ie, Escherichia coli and Shigella dysentriae) and Gram-positive bacteria (ie, Staphylococcus aureus and methicillin-resistant Staphylococcus aureus) by disk diffusion method using Mueller-Hinton agar at different sizes of Ag NPs. All of the synthesized Ag/zeolite NCs were found to have antibacterial activity. These results show that Ag NPs in the zeolite framework can be useful in different biological research and biomedical applications.

Show MeSH
Related in: MedlinePlus