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Prevention of pulmonary complications of pneumoperitoneum in rats.

Karapolat S, Gezer S, Yildirim U, Dumlu T, Karapolat B, Ozaydin I, Yasar M, Iskender A, Kandis H, Saritas A - J Cardiothorac Surg (2011)

Bottom Line: The results were statistically analyzed.A value of P < 0.05 was considered statistically significant.CO2 pneumoperitoneum results in oxidative injury to lung tissue, and administration of Erdosteine reduces the severity of pathological changes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Thoracic Surgery, Duzce University School of Medicine, Duzce, Turkey. samikarapolat@yahoo.com

ABSTRACT

Background: Carbon dioxide (CO2) pneumoperitoneum facilitates the visualization of abdominal organs during laparoscopic surgery. However, the associated increase in intra-abdominal pressure causes oxidative stress, which contributes to tissue injury.

Objective: We investigated the ability of the antioxidant and anti-inflammatory drug Erdosteine to prevent CO2 pneumoperitoneum-induced oxidative stress and inflammatory reactions in a rat model.

Methods: Fourteen female adult Wistar albino rats were divided into a control group (Group A, n = 7) and an Erdosteine group (Group B, n = 7). Group A received 0.5 cc/day 0.9% NaCl, and Group B received 10 mg/kg/day Erdosteine was administered by gavage, and maintained for 7 days prior to the operation. During the surgical procedure, the rats were exposed to CO2 pneumoperitoneum with an intra-abdominal pressure of 15 mmHg for 30 min. The peritoneal gas was then desufflated. The rats were sacrificed following 3 h of insufflation. Their lungs were removed, histologically evaluated, and scored for intra-alveolar hemorrhage, alveolar edema, congestion, and leukocyte infiltration. The results were statistically analyzed. A value of P < 0.05 was considered statistically significant.

Results: Significant differences were detected in intra-alveolar hemorrhage (P < 0.05), congestion (P < 0.001), and leukocyte infiltration (P < 0.001) in Group A compared with Group B. However, the differences in alveolar edema were not statistically significant (P = 0.698).

Conclusions: CO2 pneumoperitoneum results in oxidative injury to lung tissue, and administration of Erdosteine reduces the severity of pathological changes. Therefore, Erdosteine may be a useful preventive and therapeutic agent for CO2 pneumoperitoneum-induced oxidative stress in laparoscopic surgery.

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Photomicrograph of histopathology from Group A (Control) displaying increased intra-alveolar hemorrhage (thin short arrow), congestion (thick short arrow), and leukocyte infiltration (thick long arrow). Alveolar edema (double arrow) was slight. (Hematoxylin-Eosin, original magnification × 20)
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Figure 1: Photomicrograph of histopathology from Group A (Control) displaying increased intra-alveolar hemorrhage (thin short arrow), congestion (thick short arrow), and leukocyte infiltration (thick long arrow). Alveolar edema (double arrow) was slight. (Hematoxylin-Eosin, original magnification × 20)

Mentions: Analysis of the specimens from Group A revealed diffuse intra-alveolar hemorrhage. In addition, dense congestion and leukocyte infiltration were present. Slight alveolar edema was detected around the congestion areas. Analysis of Group B specimens showed less intra-alveolar hemorrhage, congestion, and leukocyte infiltration, especially in alveolar subepithelial regions. Overall, alveolar edema in this group was almost the same as Group A. Histopathological photographs of the sections are shown in Figures 1 &2.


Prevention of pulmonary complications of pneumoperitoneum in rats.

Karapolat S, Gezer S, Yildirim U, Dumlu T, Karapolat B, Ozaydin I, Yasar M, Iskender A, Kandis H, Saritas A - J Cardiothorac Surg (2011)

Photomicrograph of histopathology from Group A (Control) displaying increased intra-alveolar hemorrhage (thin short arrow), congestion (thick short arrow), and leukocyte infiltration (thick long arrow). Alveolar edema (double arrow) was slight. (Hematoxylin-Eosin, original magnification × 20)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3044100&req=5

Figure 1: Photomicrograph of histopathology from Group A (Control) displaying increased intra-alveolar hemorrhage (thin short arrow), congestion (thick short arrow), and leukocyte infiltration (thick long arrow). Alveolar edema (double arrow) was slight. (Hematoxylin-Eosin, original magnification × 20)
Mentions: Analysis of the specimens from Group A revealed diffuse intra-alveolar hemorrhage. In addition, dense congestion and leukocyte infiltration were present. Slight alveolar edema was detected around the congestion areas. Analysis of Group B specimens showed less intra-alveolar hemorrhage, congestion, and leukocyte infiltration, especially in alveolar subepithelial regions. Overall, alveolar edema in this group was almost the same as Group A. Histopathological photographs of the sections are shown in Figures 1 &2.

Bottom Line: The results were statistically analyzed.A value of P < 0.05 was considered statistically significant.CO2 pneumoperitoneum results in oxidative injury to lung tissue, and administration of Erdosteine reduces the severity of pathological changes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Thoracic Surgery, Duzce University School of Medicine, Duzce, Turkey. samikarapolat@yahoo.com

ABSTRACT

Background: Carbon dioxide (CO2) pneumoperitoneum facilitates the visualization of abdominal organs during laparoscopic surgery. However, the associated increase in intra-abdominal pressure causes oxidative stress, which contributes to tissue injury.

Objective: We investigated the ability of the antioxidant and anti-inflammatory drug Erdosteine to prevent CO2 pneumoperitoneum-induced oxidative stress and inflammatory reactions in a rat model.

Methods: Fourteen female adult Wistar albino rats were divided into a control group (Group A, n = 7) and an Erdosteine group (Group B, n = 7). Group A received 0.5 cc/day 0.9% NaCl, and Group B received 10 mg/kg/day Erdosteine was administered by gavage, and maintained for 7 days prior to the operation. During the surgical procedure, the rats were exposed to CO2 pneumoperitoneum with an intra-abdominal pressure of 15 mmHg for 30 min. The peritoneal gas was then desufflated. The rats were sacrificed following 3 h of insufflation. Their lungs were removed, histologically evaluated, and scored for intra-alveolar hemorrhage, alveolar edema, congestion, and leukocyte infiltration. The results were statistically analyzed. A value of P < 0.05 was considered statistically significant.

Results: Significant differences were detected in intra-alveolar hemorrhage (P < 0.05), congestion (P < 0.001), and leukocyte infiltration (P < 0.001) in Group A compared with Group B. However, the differences in alveolar edema were not statistically significant (P = 0.698).

Conclusions: CO2 pneumoperitoneum results in oxidative injury to lung tissue, and administration of Erdosteine reduces the severity of pathological changes. Therefore, Erdosteine may be a useful preventive and therapeutic agent for CO2 pneumoperitoneum-induced oxidative stress in laparoscopic surgery.

Show MeSH
Related in: MedlinePlus