Limits...
Review of matrix metalloproteinases' effect on the hybrid dentin bond layer stability and chlorhexidine clinical use to prevent bond failure.

Moon PC, Weaver J, Brooks CN - Open Dent J (2010)

Bottom Line: Dentin Bonding procedure utilizing Chlorhexidine for different application times and concentrations are being developed.The application of 2% Chlorhexidine to the phosphoric acid etch surface after rinsing off the acid is the only procedure that has been clinically tested for a longer period of time and shown to prevent bond strength degradation so far.The adoption of this procedure is recommended as means of improving bond stability at this time.

View Article: PubMed Central - PubMed

Affiliation: Department of General Practice, VCU School of Dentistry, Virginia Commonwealth University, Richmond, Va. USA.

ABSTRACT
This review describes the relationship between dentin collagen hybrid bond layer degradation and the Matrix Metalloproteinases (MMPs) after their release by acid etch and rinse adhesives and self etching bonding adhesives that can reduce the bond stability over time. MMP-2, MMP-8 and MMP-9 are indicated as the active proteases that breakdown the collagen fibrils in the hybrid bond layer. Phosphoric acid in the acid etch and rinse bonding process and acid primers in the self etch process are implicated in the release of these proteases and their activation by several non-collagen proteins also released from dentin by the etching. MMPs are released in saliva by salivary glands, by cells in the gingival crevices to crevicular fluid and by pulpal odontoblasts cells to the dentinal fluids. These sources may affect the hybrid layer also. Evidence of the bond strength deterioration over time and the ability of Chlorhexidine to prevent bond deterioration by inhibiting MMP action are discussed. Dentin Bonding procedure utilizing Chlorhexidine for different application times and concentrations are being developed. The application of 2% Chlorhexidine to the phosphoric acid etch surface after rinsing off the acid is the only procedure that has been clinically tested for a longer period of time and shown to prevent bond strength degradation so far. The adoption of this procedure is recommended as means of improving bond stability at this time.

No MeSH data available.


Related in: MedlinePlus

Chart of shear bond strengths 24 hours after bonding of OptiBond Solo Plus to enamel and dentin with and without the application of 2% chlorhexidine to bond Filtek Z250 composite to extracted teeth.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3040992&req=5

Figure 1: Chart of shear bond strengths 24 hours after bonding of OptiBond Solo Plus to enamel and dentin with and without the application of 2% chlorhexidine to bond Filtek Z250 composite to extracted teeth.

Mentions: We initially measured an increase in shear bond strength of 24 % with 2% CHX applied to etch and rinse dentin after water rinsing [45]. The composite cylinder bond was formed against a recently extracted molar tooth (n=10) whose dentin was prepared flat by a diamond saw, treated with and without 2% Chlorhexidine after acid etching. The bonding procedure to dentin used Scotch Bond (3M ESPE St. Paul, MN. USA) phosphoric acid etchant for 15 sec., rinsing 15 sec. and drying to moist dentin before applying the 2% Chlorhexidine (Consepsis, Ultradent Inc., South Jordan, UT. USA) for one minute with redrying to a moist dentin surface. Next, two coats of Optibond Bond Solo Plus were applied with 5 seconds of air drying after each before being visible light cured (VLC) for twenty seconds to which a 2mm high cylinder of Filtek Z250 (3M ESPE St. Paul, MN. USA) was placed using an Ultradent bonding jig. The Filtek Z250 was visible light cured (VLC) for forty seconds. The VLC were performed at 400 mw/cm2 with an Optilux Model VCL 401 curing light (Kerr Inc., Danbury Ct. USA). The average shearbond strength to dentin increased from 19.4 +/- 1.4 MPa for the control without chlorhexidine to 24.1 +/- 3.1 MPa with 2% Chlorhexidine after storage in water at 100of for 24 hours as listed in Fig. (1). Retesting this procedure but with 10 seconds of air pressure drying after application each layer raised the bond strength at 24 hours to 29.6 +/- 4.6 MPa without CHX and to 30.0 +/- 4.1 MPa with CHX. We now use clinically 2% Chlorhexidine with OptiBond Solo Plus for bonded direct composite restorations as of the Fall of 2007 in Virginia Commonwealth University Dental School Clinic. We started using Optibond Solo Plus in 2005 as it was available in unidose which we judged from experience would make student bonding procedures more effective and efficient.


Review of matrix metalloproteinases' effect on the hybrid dentin bond layer stability and chlorhexidine clinical use to prevent bond failure.

Moon PC, Weaver J, Brooks CN - Open Dent J (2010)

Chart of shear bond strengths 24 hours after bonding of OptiBond Solo Plus to enamel and dentin with and without the application of 2% chlorhexidine to bond Filtek Z250 composite to extracted teeth.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040992&req=5

Figure 1: Chart of shear bond strengths 24 hours after bonding of OptiBond Solo Plus to enamel and dentin with and without the application of 2% chlorhexidine to bond Filtek Z250 composite to extracted teeth.
Mentions: We initially measured an increase in shear bond strength of 24 % with 2% CHX applied to etch and rinse dentin after water rinsing [45]. The composite cylinder bond was formed against a recently extracted molar tooth (n=10) whose dentin was prepared flat by a diamond saw, treated with and without 2% Chlorhexidine after acid etching. The bonding procedure to dentin used Scotch Bond (3M ESPE St. Paul, MN. USA) phosphoric acid etchant for 15 sec., rinsing 15 sec. and drying to moist dentin before applying the 2% Chlorhexidine (Consepsis, Ultradent Inc., South Jordan, UT. USA) for one minute with redrying to a moist dentin surface. Next, two coats of Optibond Bond Solo Plus were applied with 5 seconds of air drying after each before being visible light cured (VLC) for twenty seconds to which a 2mm high cylinder of Filtek Z250 (3M ESPE St. Paul, MN. USA) was placed using an Ultradent bonding jig. The Filtek Z250 was visible light cured (VLC) for forty seconds. The VLC were performed at 400 mw/cm2 with an Optilux Model VCL 401 curing light (Kerr Inc., Danbury Ct. USA). The average shearbond strength to dentin increased from 19.4 +/- 1.4 MPa for the control without chlorhexidine to 24.1 +/- 3.1 MPa with 2% Chlorhexidine after storage in water at 100of for 24 hours as listed in Fig. (1). Retesting this procedure but with 10 seconds of air pressure drying after application each layer raised the bond strength at 24 hours to 29.6 +/- 4.6 MPa without CHX and to 30.0 +/- 4.1 MPa with CHX. We now use clinically 2% Chlorhexidine with OptiBond Solo Plus for bonded direct composite restorations as of the Fall of 2007 in Virginia Commonwealth University Dental School Clinic. We started using Optibond Solo Plus in 2005 as it was available in unidose which we judged from experience would make student bonding procedures more effective and efficient.

Bottom Line: Dentin Bonding procedure utilizing Chlorhexidine for different application times and concentrations are being developed.The application of 2% Chlorhexidine to the phosphoric acid etch surface after rinsing off the acid is the only procedure that has been clinically tested for a longer period of time and shown to prevent bond strength degradation so far.The adoption of this procedure is recommended as means of improving bond stability at this time.

View Article: PubMed Central - PubMed

Affiliation: Department of General Practice, VCU School of Dentistry, Virginia Commonwealth University, Richmond, Va. USA.

ABSTRACT
This review describes the relationship between dentin collagen hybrid bond layer degradation and the Matrix Metalloproteinases (MMPs) after their release by acid etch and rinse adhesives and self etching bonding adhesives that can reduce the bond stability over time. MMP-2, MMP-8 and MMP-9 are indicated as the active proteases that breakdown the collagen fibrils in the hybrid bond layer. Phosphoric acid in the acid etch and rinse bonding process and acid primers in the self etch process are implicated in the release of these proteases and their activation by several non-collagen proteins also released from dentin by the etching. MMPs are released in saliva by salivary glands, by cells in the gingival crevices to crevicular fluid and by pulpal odontoblasts cells to the dentinal fluids. These sources may affect the hybrid layer also. Evidence of the bond strength deterioration over time and the ability of Chlorhexidine to prevent bond deterioration by inhibiting MMP action are discussed. Dentin Bonding procedure utilizing Chlorhexidine for different application times and concentrations are being developed. The application of 2% Chlorhexidine to the phosphoric acid etch surface after rinsing off the acid is the only procedure that has been clinically tested for a longer period of time and shown to prevent bond strength degradation so far. The adoption of this procedure is recommended as means of improving bond stability at this time.

No MeSH data available.


Related in: MedlinePlus