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Comparison of the bacterial symbiont composition of the formosan subterranean termite from its native and introduced range.

Husseneder C, Ho HY, Blackwell M - Open Microbiol J (2010)

Bottom Line: Louisiana, U.S. (introduced range) using 16S rRNA gene sequencing.Apparently, the tight co-evolutionary link between termites and their gut flora maintains a certain association of species and functional groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.

ABSTRACT
We investigated the bacterial composition in the gut of Formosan subterranean termites (FST), Coptotermes formosanus Shiraki, collected from southern China (native range) vs. Louisiana, U. S. (introduced range) using 16S rRNA gene sequencing. Overall, we identified 213 bacteria ribotypes from thirteen phyla. The enemy release hypothesis could not be invoked to explain invasion success of FST since no pathogens were found among the bacterial gut community regardless of geographic origin. Invasion of new habitats did not significantly change the bacteria composition. Apparently, the tight co-evolutionary link between termites and their gut flora maintains a certain association of species and functional groups. Ribotype richness, bacteria diversity, and proportions of detected phyla were not influenced by geographic origin of FST samples; however, these parameters were affected by storage of the samples. Ethanol storage of termite samples (5 yrs) increased the relative proportions of gram-positive bacteria versus gram-negative bacteria.

No MeSH data available.


Related in: MedlinePlus

Proportions of the four major phyla (>5%) and other minor phyla (<5%) in fresh and EtOH-stored FST samples from the native range in China and the introduced range in Louisiana (LA). Previously published data from an FST sample from Japan were included for comparison [14].
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Figure 3: Proportions of the four major phyla (>5%) and other minor phyla (<5%) in fresh and EtOH-stored FST samples from the native range in China and the introduced range in Louisiana (LA). Previously published data from an FST sample from Japan were included for comparison [14].

Mentions: The proportion of the most abundant phylum Bacteroides, was significantly higher in the fresh samples than in the EtOH-stored samples (p = 0.01, Chi-Square = 6.585, df = 1, Kruskal-Wallis ANOVA, Fig. 3). The Spirochaetes were marginally more abundant in fresh samples (p = 0.05, Chi-Square = 3.70, df = 1). On the contrary, the gram-positive phyla Firmicutes and Actinobacteria were significantly more abundant in the EtOH stored samples than in fresh samples (p = 0.01, Chi-Square = 6.59, df = 1 for both phyla). When LA samples were considered separately from the other geographic locations the proportions of Firmicutes and Actinobacteria were still significantly higher in EtOH-stored samples (p = 0.04, Chi-Square = 3.97, df = 1) while Bacteroides and Spirochaeta were marginally higher in fresh samples (p = 0.05, Chi-Square = 3.85, df = 1, Fig. 3).


Comparison of the bacterial symbiont composition of the formosan subterranean termite from its native and introduced range.

Husseneder C, Ho HY, Blackwell M - Open Microbiol J (2010)

Proportions of the four major phyla (>5%) and other minor phyla (<5%) in fresh and EtOH-stored FST samples from the native range in China and the introduced range in Louisiana (LA). Previously published data from an FST sample from Japan were included for comparison [14].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040989&req=5

Figure 3: Proportions of the four major phyla (>5%) and other minor phyla (<5%) in fresh and EtOH-stored FST samples from the native range in China and the introduced range in Louisiana (LA). Previously published data from an FST sample from Japan were included for comparison [14].
Mentions: The proportion of the most abundant phylum Bacteroides, was significantly higher in the fresh samples than in the EtOH-stored samples (p = 0.01, Chi-Square = 6.585, df = 1, Kruskal-Wallis ANOVA, Fig. 3). The Spirochaetes were marginally more abundant in fresh samples (p = 0.05, Chi-Square = 3.70, df = 1). On the contrary, the gram-positive phyla Firmicutes and Actinobacteria were significantly more abundant in the EtOH stored samples than in fresh samples (p = 0.01, Chi-Square = 6.59, df = 1 for both phyla). When LA samples were considered separately from the other geographic locations the proportions of Firmicutes and Actinobacteria were still significantly higher in EtOH-stored samples (p = 0.04, Chi-Square = 3.97, df = 1) while Bacteroides and Spirochaeta were marginally higher in fresh samples (p = 0.05, Chi-Square = 3.85, df = 1, Fig. 3).

Bottom Line: Louisiana, U.S. (introduced range) using 16S rRNA gene sequencing.Apparently, the tight co-evolutionary link between termites and their gut flora maintains a certain association of species and functional groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.

ABSTRACT
We investigated the bacterial composition in the gut of Formosan subterranean termites (FST), Coptotermes formosanus Shiraki, collected from southern China (native range) vs. Louisiana, U. S. (introduced range) using 16S rRNA gene sequencing. Overall, we identified 213 bacteria ribotypes from thirteen phyla. The enemy release hypothesis could not be invoked to explain invasion success of FST since no pathogens were found among the bacterial gut community regardless of geographic origin. Invasion of new habitats did not significantly change the bacteria composition. Apparently, the tight co-evolutionary link between termites and their gut flora maintains a certain association of species and functional groups. Ribotype richness, bacteria diversity, and proportions of detected phyla were not influenced by geographic origin of FST samples; however, these parameters were affected by storage of the samples. Ethanol storage of termite samples (5 yrs) increased the relative proportions of gram-positive bacteria versus gram-negative bacteria.

No MeSH data available.


Related in: MedlinePlus