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Comparison of the bacterial symbiont composition of the formosan subterranean termite from its native and introduced range.

Husseneder C, Ho HY, Blackwell M - Open Microbiol J (2010)

Bottom Line: Louisiana, U.S. (introduced range) using 16S rRNA gene sequencing.Apparently, the tight co-evolutionary link between termites and their gut flora maintains a certain association of species and functional groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.

ABSTRACT
We investigated the bacterial composition in the gut of Formosan subterranean termites (FST), Coptotermes formosanus Shiraki, collected from southern China (native range) vs. Louisiana, U. S. (introduced range) using 16S rRNA gene sequencing. Overall, we identified 213 bacteria ribotypes from thirteen phyla. The enemy release hypothesis could not be invoked to explain invasion success of FST since no pathogens were found among the bacterial gut community regardless of geographic origin. Invasion of new habitats did not significantly change the bacteria composition. Apparently, the tight co-evolutionary link between termites and their gut flora maintains a certain association of species and functional groups. Ribotype richness, bacteria diversity, and proportions of detected phyla were not influenced by geographic origin of FST samples; however, these parameters were affected by storage of the samples. Ethanol storage of termite samples (5 yrs) increased the relative proportions of gram-positive bacteria versus gram-negative bacteria.

No MeSH data available.


Related in: MedlinePlus

Rarefaction curves of bacterial phyla and ribotypes depending upon the number of FST samples. Observed richness of phyla and ribotypes (Sob) was measured based on the Mao Tau index. Total richness was estimated using Chao 1. Upper and lower 95% confidence intervals are given.
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Figure 2: Rarefaction curves of bacterial phyla and ribotypes depending upon the number of FST samples. Observed richness of phyla and ribotypes (Sob) was measured based on the Mao Tau index. Total richness was estimated using Chao 1. Upper and lower 95% confidence intervals are given.

Mentions: The rarefaction curve of observed number of phyla depending on the number of FST samples levels off and approaches the curve of estimated total number of phyla (Fig. 2). This indicates that 10 FST samples represent sufficient sampling effort and that the 13 phyla observed capture the majority of bacterial phyla expected in the guts of FSTs. The continued slight incline of the accumulation curve of the number of ribotypes, however, suggests that more ribotypes might be discovered if further FST samples would be analysed.The 213 ribotypes represent at least 55% to 77% ofthe estimated total number of ribotypes (Fig. 2).


Comparison of the bacterial symbiont composition of the formosan subterranean termite from its native and introduced range.

Husseneder C, Ho HY, Blackwell M - Open Microbiol J (2010)

Rarefaction curves of bacterial phyla and ribotypes depending upon the number of FST samples. Observed richness of phyla and ribotypes (Sob) was measured based on the Mao Tau index. Total richness was estimated using Chao 1. Upper and lower 95% confidence intervals are given.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040989&req=5

Figure 2: Rarefaction curves of bacterial phyla and ribotypes depending upon the number of FST samples. Observed richness of phyla and ribotypes (Sob) was measured based on the Mao Tau index. Total richness was estimated using Chao 1. Upper and lower 95% confidence intervals are given.
Mentions: The rarefaction curve of observed number of phyla depending on the number of FST samples levels off and approaches the curve of estimated total number of phyla (Fig. 2). This indicates that 10 FST samples represent sufficient sampling effort and that the 13 phyla observed capture the majority of bacterial phyla expected in the guts of FSTs. The continued slight incline of the accumulation curve of the number of ribotypes, however, suggests that more ribotypes might be discovered if further FST samples would be analysed.The 213 ribotypes represent at least 55% to 77% ofthe estimated total number of ribotypes (Fig. 2).

Bottom Line: Louisiana, U.S. (introduced range) using 16S rRNA gene sequencing.Apparently, the tight co-evolutionary link between termites and their gut flora maintains a certain association of species and functional groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.

ABSTRACT
We investigated the bacterial composition in the gut of Formosan subterranean termites (FST), Coptotermes formosanus Shiraki, collected from southern China (native range) vs. Louisiana, U. S. (introduced range) using 16S rRNA gene sequencing. Overall, we identified 213 bacteria ribotypes from thirteen phyla. The enemy release hypothesis could not be invoked to explain invasion success of FST since no pathogens were found among the bacterial gut community regardless of geographic origin. Invasion of new habitats did not significantly change the bacteria composition. Apparently, the tight co-evolutionary link between termites and their gut flora maintains a certain association of species and functional groups. Ribotype richness, bacteria diversity, and proportions of detected phyla were not influenced by geographic origin of FST samples; however, these parameters were affected by storage of the samples. Ethanol storage of termite samples (5 yrs) increased the relative proportions of gram-positive bacteria versus gram-negative bacteria.

No MeSH data available.


Related in: MedlinePlus