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Comparison of the bacterial symbiont composition of the formosan subterranean termite from its native and introduced range.

Husseneder C, Ho HY, Blackwell M - Open Microbiol J (2010)

Bottom Line: Louisiana, U.S. (introduced range) using 16S rRNA gene sequencing.Apparently, the tight co-evolutionary link between termites and their gut flora maintains a certain association of species and functional groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.

ABSTRACT
We investigated the bacterial composition in the gut of Formosan subterranean termites (FST), Coptotermes formosanus Shiraki, collected from southern China (native range) vs. Louisiana, U. S. (introduced range) using 16S rRNA gene sequencing. Overall, we identified 213 bacteria ribotypes from thirteen phyla. The enemy release hypothesis could not be invoked to explain invasion success of FST since no pathogens were found among the bacterial gut community regardless of geographic origin. Invasion of new habitats did not significantly change the bacteria composition. Apparently, the tight co-evolutionary link between termites and their gut flora maintains a certain association of species and functional groups. Ribotype richness, bacteria diversity, and proportions of detected phyla were not influenced by geographic origin of FST samples; however, these parameters were affected by storage of the samples. Ethanol storage of termite samples (5 yrs) increased the relative proportions of gram-positive bacteria versus gram-negative bacteria.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic trees constructed from the 16S rRNA gene sequences of gut bacteria of Formosan subterranean termite (FST) samplesfrom Louisiana and China (Cf) and their highest match from the DDBJ/EMBL/GenBank databases. A. Bacteroidetes, B. Firmicutes, C.Spirochaetes, D. Actinobacteria, E. Proteobacteria, F. TM7, G. Verrucomicrobia and Planctomycetes. The phylogenetic trees wereconstructed using the neighbor joining method with 1000 bootstrap replicates. Only bootstrap values of ≥50 are indicated on the branchnodes. The scale bars represent 2% difference in nucleotide sequence. Closely related ribotype sequences from other studies were included forcomparison and marked with circles (● termite-specific bacteria, ○ environmental bacteria). A. Bacteroidetes. B. Firmicutes & Synergistes.C. Spirochaetes. D. Proteobacteria. E. Actinobacteria. F. TM7. G. Planctomycetes & Verrucomicrobia.
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Figure 1: Phylogenetic trees constructed from the 16S rRNA gene sequences of gut bacteria of Formosan subterranean termite (FST) samplesfrom Louisiana and China (Cf) and their highest match from the DDBJ/EMBL/GenBank databases. A. Bacteroidetes, B. Firmicutes, C.Spirochaetes, D. Actinobacteria, E. Proteobacteria, F. TM7, G. Verrucomicrobia and Planctomycetes. The phylogenetic trees wereconstructed using the neighbor joining method with 1000 bootstrap replicates. Only bootstrap values of ≥50 are indicated on the branchnodes. The scale bars represent 2% difference in nucleotide sequence. Closely related ribotype sequences from other studies were included forcomparison and marked with circles (● termite-specific bacteria, ○ environmental bacteria). A. Bacteroidetes. B. Firmicutes & Synergistes.C. Spirochaetes. D. Proteobacteria. E. Actinobacteria. F. TM7. G. Planctomycetes & Verrucomicrobia.

Mentions: Using the default settings on the naïve Bayesian rRNA classifier [39] on the RDP website [40], the sequences were classified into the known major bacteria lineages based on a confidence level of 80%. The ribotypes were named according to the prefix Cf (Coptotermes formosanus) followed by a first numeral indicating the phylum classification of the ribotype on the RDP website and a second numeral assigned to each ribotype. Classification of the sequences was confirmed using BLAST and reclassified based on the latest publications. Sequence divergence among the ribotypes was calculated using the Kimura 2-parameter model. Phylogenetic trees were constructed using the neighbor-joining method as implemented in MEGA 4.0 with 1000 bootstrap replicates (Fig. 1).


Comparison of the bacterial symbiont composition of the formosan subterranean termite from its native and introduced range.

Husseneder C, Ho HY, Blackwell M - Open Microbiol J (2010)

Phylogenetic trees constructed from the 16S rRNA gene sequences of gut bacteria of Formosan subterranean termite (FST) samplesfrom Louisiana and China (Cf) and their highest match from the DDBJ/EMBL/GenBank databases. A. Bacteroidetes, B. Firmicutes, C.Spirochaetes, D. Actinobacteria, E. Proteobacteria, F. TM7, G. Verrucomicrobia and Planctomycetes. The phylogenetic trees wereconstructed using the neighbor joining method with 1000 bootstrap replicates. Only bootstrap values of ≥50 are indicated on the branchnodes. The scale bars represent 2% difference in nucleotide sequence. Closely related ribotype sequences from other studies were included forcomparison and marked with circles (● termite-specific bacteria, ○ environmental bacteria). A. Bacteroidetes. B. Firmicutes & Synergistes.C. Spirochaetes. D. Proteobacteria. E. Actinobacteria. F. TM7. G. Planctomycetes & Verrucomicrobia.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040989&req=5

Figure 1: Phylogenetic trees constructed from the 16S rRNA gene sequences of gut bacteria of Formosan subterranean termite (FST) samplesfrom Louisiana and China (Cf) and their highest match from the DDBJ/EMBL/GenBank databases. A. Bacteroidetes, B. Firmicutes, C.Spirochaetes, D. Actinobacteria, E. Proteobacteria, F. TM7, G. Verrucomicrobia and Planctomycetes. The phylogenetic trees wereconstructed using the neighbor joining method with 1000 bootstrap replicates. Only bootstrap values of ≥50 are indicated on the branchnodes. The scale bars represent 2% difference in nucleotide sequence. Closely related ribotype sequences from other studies were included forcomparison and marked with circles (● termite-specific bacteria, ○ environmental bacteria). A. Bacteroidetes. B. Firmicutes & Synergistes.C. Spirochaetes. D. Proteobacteria. E. Actinobacteria. F. TM7. G. Planctomycetes & Verrucomicrobia.
Mentions: Using the default settings on the naïve Bayesian rRNA classifier [39] on the RDP website [40], the sequences were classified into the known major bacteria lineages based on a confidence level of 80%. The ribotypes were named according to the prefix Cf (Coptotermes formosanus) followed by a first numeral indicating the phylum classification of the ribotype on the RDP website and a second numeral assigned to each ribotype. Classification of the sequences was confirmed using BLAST and reclassified based on the latest publications. Sequence divergence among the ribotypes was calculated using the Kimura 2-parameter model. Phylogenetic trees were constructed using the neighbor-joining method as implemented in MEGA 4.0 with 1000 bootstrap replicates (Fig. 1).

Bottom Line: Louisiana, U.S. (introduced range) using 16S rRNA gene sequencing.Apparently, the tight co-evolutionary link between termites and their gut flora maintains a certain association of species and functional groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.

ABSTRACT
We investigated the bacterial composition in the gut of Formosan subterranean termites (FST), Coptotermes formosanus Shiraki, collected from southern China (native range) vs. Louisiana, U. S. (introduced range) using 16S rRNA gene sequencing. Overall, we identified 213 bacteria ribotypes from thirteen phyla. The enemy release hypothesis could not be invoked to explain invasion success of FST since no pathogens were found among the bacterial gut community regardless of geographic origin. Invasion of new habitats did not significantly change the bacteria composition. Apparently, the tight co-evolutionary link between termites and their gut flora maintains a certain association of species and functional groups. Ribotype richness, bacteria diversity, and proportions of detected phyla were not influenced by geographic origin of FST samples; however, these parameters were affected by storage of the samples. Ethanol storage of termite samples (5 yrs) increased the relative proportions of gram-positive bacteria versus gram-negative bacteria.

No MeSH data available.


Related in: MedlinePlus