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Estimation of Apigenin, an Anxiolytic Constituent, in Turnera aphrodisiaca.

Kumar S, Madaan R, Sharma A - Indian J Pharm Sci (2008)

Bottom Line: DWSG contained higher content of apigenin.Aerial parts of the plant were collected at bimonthly intervals over a period of one year in the months of January, March, May, July, September and November.The plant material collected in September showed maximum content of apigenin.

View Article: PubMed Central - PubMed

Affiliation: S. D. College of Pharmacy, Barnala-148 101, India.

ABSTRACT
An HPTLC densitometric method has been developed to estimate apigenin in Turnera aphrodisiaca aerial and its segregated parts (leaves, stems, flowers and fruits) so that plant can be standardized on the basis of its bioactive marker. The apigenin content in methanol extract of T. aphrodisiaca aerial parts was found to be about fourteen times less than acid hydrolyzed methanol extract of the plant indicating the presence of most of apigenin in glycosidic form. Amongst different plant parts, flowers possessed maximum content of apigenin followed by leaves. The apigenin content was also determined in three marketed formulations of T. aphrodisiaca viz., NLK, DWSG and SBL. DWSG contained higher content of apigenin. Aerial parts of the plant were collected at bimonthly intervals over a period of one year in the months of January, March, May, July, September and November. The plant material collected in September showed maximum content of apigenin.

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Related in: MedlinePlus

Recovery plot for apigenin.Plot of Apigenin (0.05, 0.10, 0.20 %w/w) added to T. aphrodisiaca aerial parts (1 g) and content of apigenin observed. Linear regression equation was y = 0.9857× + 0.2 (r2 =1).
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Figure 0003: Recovery plot for apigenin.Plot of Apigenin (0.05, 0.10, 0.20 %w/w) added to T. aphrodisiaca aerial parts (1 g) and content of apigenin observed. Linear regression equation was y = 0.9857× + 0.2 (r2 =1).

Mentions: Apigenin (0.5, 1.0 and 2.0 mg) were added to 1 g of T. aphrodisiaca aerial parts in three separate flasks. The extraction was carried out as per the procedure mentioned earlier. The resulting solutions were concentrated under reduced pressure, finally making up their volume to 50 ml. A 2 μl sample of every solution was subjected to HPTLC for the estimation of apigenin. The estimated quantity of apigenin was expressed as % w/w. The recovery graph was obtained by plotting % w/w-added versus % w/w-determined (fig. 3). Recovery % age was calculated by multiplying slope of the regression line by 100.


Estimation of Apigenin, an Anxiolytic Constituent, in Turnera aphrodisiaca.

Kumar S, Madaan R, Sharma A - Indian J Pharm Sci (2008)

Recovery plot for apigenin.Plot of Apigenin (0.05, 0.10, 0.20 %w/w) added to T. aphrodisiaca aerial parts (1 g) and content of apigenin observed. Linear regression equation was y = 0.9857× + 0.2 (r2 =1).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040895&req=5

Figure 0003: Recovery plot for apigenin.Plot of Apigenin (0.05, 0.10, 0.20 %w/w) added to T. aphrodisiaca aerial parts (1 g) and content of apigenin observed. Linear regression equation was y = 0.9857× + 0.2 (r2 =1).
Mentions: Apigenin (0.5, 1.0 and 2.0 mg) were added to 1 g of T. aphrodisiaca aerial parts in three separate flasks. The extraction was carried out as per the procedure mentioned earlier. The resulting solutions were concentrated under reduced pressure, finally making up their volume to 50 ml. A 2 μl sample of every solution was subjected to HPTLC for the estimation of apigenin. The estimated quantity of apigenin was expressed as % w/w. The recovery graph was obtained by plotting % w/w-added versus % w/w-determined (fig. 3). Recovery % age was calculated by multiplying slope of the regression line by 100.

Bottom Line: DWSG contained higher content of apigenin.Aerial parts of the plant were collected at bimonthly intervals over a period of one year in the months of January, March, May, July, September and November.The plant material collected in September showed maximum content of apigenin.

View Article: PubMed Central - PubMed

Affiliation: S. D. College of Pharmacy, Barnala-148 101, India.

ABSTRACT
An HPTLC densitometric method has been developed to estimate apigenin in Turnera aphrodisiaca aerial and its segregated parts (leaves, stems, flowers and fruits) so that plant can be standardized on the basis of its bioactive marker. The apigenin content in methanol extract of T. aphrodisiaca aerial parts was found to be about fourteen times less than acid hydrolyzed methanol extract of the plant indicating the presence of most of apigenin in glycosidic form. Amongst different plant parts, flowers possessed maximum content of apigenin followed by leaves. The apigenin content was also determined in three marketed formulations of T. aphrodisiaca viz., NLK, DWSG and SBL. DWSG contained higher content of apigenin. Aerial parts of the plant were collected at bimonthly intervals over a period of one year in the months of January, March, May, July, September and November. The plant material collected in September showed maximum content of apigenin.

No MeSH data available.


Related in: MedlinePlus