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Estimation of Apigenin, an Anxiolytic Constituent, in Turnera aphrodisiaca.

Kumar S, Madaan R, Sharma A - Indian J Pharm Sci (2008)

Bottom Line: DWSG contained higher content of apigenin.Aerial parts of the plant were collected at bimonthly intervals over a period of one year in the months of January, March, May, July, September and November.The plant material collected in September showed maximum content of apigenin.

View Article: PubMed Central - PubMed

Affiliation: S. D. College of Pharmacy, Barnala-148 101, India.

ABSTRACT
An HPTLC densitometric method has been developed to estimate apigenin in Turnera aphrodisiaca aerial and its segregated parts (leaves, stems, flowers and fruits) so that plant can be standardized on the basis of its bioactive marker. The apigenin content in methanol extract of T. aphrodisiaca aerial parts was found to be about fourteen times less than acid hydrolyzed methanol extract of the plant indicating the presence of most of apigenin in glycosidic form. Amongst different plant parts, flowers possessed maximum content of apigenin followed by leaves. The apigenin content was also determined in three marketed formulations of T. aphrodisiaca viz., NLK, DWSG and SBL. DWSG contained higher content of apigenin. Aerial parts of the plant were collected at bimonthly intervals over a period of one year in the months of January, March, May, July, September and November. The plant material collected in September showed maximum content of apigenin.

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Related in: MedlinePlus

HPTL chromatogram of apigenin.HPTL chromatogram of apigenin (0.5 mg/10 ml) from T. aphrodisiaca. Mobile phase, toluene:ethyl acetate (4:1); visualization at 336 nm.
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Figure 0001: HPTL chromatogram of apigenin.HPTL chromatogram of apigenin (0.5 mg/10 ml) from T. aphrodisiaca. Mobile phase, toluene:ethyl acetate (4:1); visualization at 336 nm.

Mentions: Two microlitres each of the stock solution and the four working standard solutions of apigenin were applied, in triplicate, on 20×20 cm TLC plates. The developed plates were scanned at 336 nm using HPTLC densitometer. Fig. 1 shows the HPTL chromatogram of apigenin from T. aphrodisiaca. A standard graph was plotted against mean area under the peak and apigenin amount (μg). Aliquots (2 μl) of test samples were applied, in triplicate, to the TLC plates. Fig. 2 shows a representative HPTL chromatogram of acid hydrolyzed methanol extract of aerial parts of T. aphrodisiaca. The area under the curve of every sample was recorded, and apigenin content was determined from the regression equation of the standard graph.


Estimation of Apigenin, an Anxiolytic Constituent, in Turnera aphrodisiaca.

Kumar S, Madaan R, Sharma A - Indian J Pharm Sci (2008)

HPTL chromatogram of apigenin.HPTL chromatogram of apigenin (0.5 mg/10 ml) from T. aphrodisiaca. Mobile phase, toluene:ethyl acetate (4:1); visualization at 336 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040895&req=5

Figure 0001: HPTL chromatogram of apigenin.HPTL chromatogram of apigenin (0.5 mg/10 ml) from T. aphrodisiaca. Mobile phase, toluene:ethyl acetate (4:1); visualization at 336 nm.
Mentions: Two microlitres each of the stock solution and the four working standard solutions of apigenin were applied, in triplicate, on 20×20 cm TLC plates. The developed plates were scanned at 336 nm using HPTLC densitometer. Fig. 1 shows the HPTL chromatogram of apigenin from T. aphrodisiaca. A standard graph was plotted against mean area under the peak and apigenin amount (μg). Aliquots (2 μl) of test samples were applied, in triplicate, to the TLC plates. Fig. 2 shows a representative HPTL chromatogram of acid hydrolyzed methanol extract of aerial parts of T. aphrodisiaca. The area under the curve of every sample was recorded, and apigenin content was determined from the regression equation of the standard graph.

Bottom Line: DWSG contained higher content of apigenin.Aerial parts of the plant were collected at bimonthly intervals over a period of one year in the months of January, March, May, July, September and November.The plant material collected in September showed maximum content of apigenin.

View Article: PubMed Central - PubMed

Affiliation: S. D. College of Pharmacy, Barnala-148 101, India.

ABSTRACT
An HPTLC densitometric method has been developed to estimate apigenin in Turnera aphrodisiaca aerial and its segregated parts (leaves, stems, flowers and fruits) so that plant can be standardized on the basis of its bioactive marker. The apigenin content in methanol extract of T. aphrodisiaca aerial parts was found to be about fourteen times less than acid hydrolyzed methanol extract of the plant indicating the presence of most of apigenin in glycosidic form. Amongst different plant parts, flowers possessed maximum content of apigenin followed by leaves. The apigenin content was also determined in three marketed formulations of T. aphrodisiaca viz., NLK, DWSG and SBL. DWSG contained higher content of apigenin. Aerial parts of the plant were collected at bimonthly intervals over a period of one year in the months of January, March, May, July, September and November. The plant material collected in September showed maximum content of apigenin.

No MeSH data available.


Related in: MedlinePlus