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A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica.

Unnikrishnan KP, Raja SS, Balachandran I - Indian J Pharm Sci (2008)

Bottom Line: A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica.P. indica was found to contain significantly higher amount of plumbagin than P. zeylanica.The HPLC and HPTLC methods described here are simple, rapid, accurate and sensitive.

View Article: PubMed Central - PubMed

Affiliation: Centre for Medicinal Plants Research, Arya Vaidya Sala, Kottakkal, Malappuram-676 503, India.

ABSTRACT
A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica. A quantitative HPTLC method was also developed using hexane: ethyl acetate (8:2) as the mobile phase. The plumbagin content in the roots were determined using both the methods. P. indica was found to contain significantly higher amount of plumbagin than P. zeylanica. The HPLC and HPTLC methods described here are simple, rapid, accurate and sensitive.

No MeSH data available.


Typical HPTLC chromatograms of plumbagin. Typical HPTLC chromatograms for analysis of Plumbagin. (A) Plumbagin standard, (B) P. indica, (C) P. zeylanica
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Figure 0002: Typical HPTLC chromatograms of plumbagin. Typical HPTLC chromatograms for analysis of Plumbagin. (A) Plumbagin standard, (B) P. indica, (C) P. zeylanica

Mentions: With respect to HPTLC analysis, among the various solvent systems tried, the mobile phase that gave desired resolution with symmetrical and reproducible peaks was n-hexane: ethyl acetate (8:2). The use of this solvent system provides good separation of plumbagin with good resolution and separation from the other constituents of the plants. Using the proposed HPTLC method, Rf of plumbagin was determined as 0.67. The spectral characteristics of the peak at Rf 0.67 were also found to match, indicating that the compound corresponding to this Rf of the standard and test samples was identical. The chromatogram of standard plumbagin and that of test sample is shown in (fig. 2). The percentage of bioactive marker present in P. indica and P. zeylanica were determined to be 0.1921 and 0.1455%, respectively. The calibration curve was found to be linear in the range 1-20 μg.


A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica.

Unnikrishnan KP, Raja SS, Balachandran I - Indian J Pharm Sci (2008)

Typical HPTLC chromatograms of plumbagin. Typical HPTLC chromatograms for analysis of Plumbagin. (A) Plumbagin standard, (B) P. indica, (C) P. zeylanica
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040894&req=5

Figure 0002: Typical HPTLC chromatograms of plumbagin. Typical HPTLC chromatograms for analysis of Plumbagin. (A) Plumbagin standard, (B) P. indica, (C) P. zeylanica
Mentions: With respect to HPTLC analysis, among the various solvent systems tried, the mobile phase that gave desired resolution with symmetrical and reproducible peaks was n-hexane: ethyl acetate (8:2). The use of this solvent system provides good separation of plumbagin with good resolution and separation from the other constituents of the plants. Using the proposed HPTLC method, Rf of plumbagin was determined as 0.67. The spectral characteristics of the peak at Rf 0.67 were also found to match, indicating that the compound corresponding to this Rf of the standard and test samples was identical. The chromatogram of standard plumbagin and that of test sample is shown in (fig. 2). The percentage of bioactive marker present in P. indica and P. zeylanica were determined to be 0.1921 and 0.1455%, respectively. The calibration curve was found to be linear in the range 1-20 μg.

Bottom Line: A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica.P. indica was found to contain significantly higher amount of plumbagin than P. zeylanica.The HPLC and HPTLC methods described here are simple, rapid, accurate and sensitive.

View Article: PubMed Central - PubMed

Affiliation: Centre for Medicinal Plants Research, Arya Vaidya Sala, Kottakkal, Malappuram-676 503, India.

ABSTRACT
A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica. A quantitative HPTLC method was also developed using hexane: ethyl acetate (8:2) as the mobile phase. The plumbagin content in the roots were determined using both the methods. P. indica was found to contain significantly higher amount of plumbagin than P. zeylanica. The HPLC and HPTLC methods described here are simple, rapid, accurate and sensitive.

No MeSH data available.