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A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica.

Unnikrishnan KP, Raja SS, Balachandran I - Indian J Pharm Sci (2008)

Bottom Line: A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica.P. indica was found to contain significantly higher amount of plumbagin than P. zeylanica.The HPLC and HPTLC methods described here are simple, rapid, accurate and sensitive.

View Article: PubMed Central - PubMed

Affiliation: Centre for Medicinal Plants Research, Arya Vaidya Sala, Kottakkal, Malappuram-676 503, India.

ABSTRACT
A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica. A quantitative HPTLC method was also developed using hexane: ethyl acetate (8:2) as the mobile phase. The plumbagin content in the roots were determined using both the methods. P. indica was found to contain significantly higher amount of plumbagin than P. zeylanica. The HPLC and HPTLC methods described here are simple, rapid, accurate and sensitive.

No MeSH data available.


Typical HPLC chromatograms of plumbagin. Typical HPLC chromatograms for analysis of Plumbagin. (A) Plumbagin standard, (B) P. indica, (C) P. zeylanica
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Figure 0001: Typical HPLC chromatograms of plumbagin. Typical HPLC chromatograms for analysis of Plumbagin. (A) Plumbagin standard, (B) P. indica, (C) P. zeylanica

Mentions: A reverse phase C-18 column with the mobile phase methanol and sodium dihydrogen phosphate (9:1 v/v) provided good separation of plumbagin from the herbal extracts (fig. 1). The detection wavelength was maintained at 265 nm as plumbagin showed good UV absorption in this region with no interference from the mobile phase or other components of the extract. The retention time of plumbagin was 4.21 min. The content of plumbagin in P. indica and P. zeylanica was found to be 0.2001 and 0.1601 %, respectively


A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica.

Unnikrishnan KP, Raja SS, Balachandran I - Indian J Pharm Sci (2008)

Typical HPLC chromatograms of plumbagin. Typical HPLC chromatograms for analysis of Plumbagin. (A) Plumbagin standard, (B) P. indica, (C) P. zeylanica
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040894&req=5

Figure 0001: Typical HPLC chromatograms of plumbagin. Typical HPLC chromatograms for analysis of Plumbagin. (A) Plumbagin standard, (B) P. indica, (C) P. zeylanica
Mentions: A reverse phase C-18 column with the mobile phase methanol and sodium dihydrogen phosphate (9:1 v/v) provided good separation of plumbagin from the herbal extracts (fig. 1). The detection wavelength was maintained at 265 nm as plumbagin showed good UV absorption in this region with no interference from the mobile phase or other components of the extract. The retention time of plumbagin was 4.21 min. The content of plumbagin in P. indica and P. zeylanica was found to be 0.2001 and 0.1601 %, respectively

Bottom Line: A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica.P. indica was found to contain significantly higher amount of plumbagin than P. zeylanica.The HPLC and HPTLC methods described here are simple, rapid, accurate and sensitive.

View Article: PubMed Central - PubMed

Affiliation: Centre for Medicinal Plants Research, Arya Vaidya Sala, Kottakkal, Malappuram-676 503, India.

ABSTRACT
A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica. A quantitative HPTLC method was also developed using hexane: ethyl acetate (8:2) as the mobile phase. The plumbagin content in the roots were determined using both the methods. P. indica was found to contain significantly higher amount of plumbagin than P. zeylanica. The HPLC and HPTLC methods described here are simple, rapid, accurate and sensitive.

No MeSH data available.