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Development and Validation of a HPTLC Method for the Estimation of Sumatriptan in Tablet Dosage Forms.

Shah CR, Suhagia BN, Shah NJ, Shah RR - Indian J Pharm Sci (2008)

Bottom Line: The stationary phase used was precoated silica gel 60F254.The mobile phase used was a mixture of methanol:water:glacial acetic acid (4.0:8.0:0.1, v/v/v).The limit of detection and the limit of quantification for the sumatriptan were found to be 63.87 and 193.54 ng/spot, respectively.

View Article: PubMed Central - PubMed

Affiliation: Shri B. M. Shah College of Pharmaceutical Education and Research, Modasa-383 315, India.

ABSTRACT
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of sumatriptan in tablet dosage forms. The stationary phase used was precoated silica gel 60F254. The mobile phase used was a mixture of methanol:water:glacial acetic acid (4.0:8.0:0.1, v/v/v). The detection of spots was carried out at 230 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 800 ng/spot. The limit of detection and the limit of quantification for the sumatriptan were found to be 63.87 and 193.54 ng/spot, respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.

No MeSH data available.


Related in: MedlinePlus

A typical HPTLC Chromatogram of SumatriptanHPTLC chromatogram represents peak of sumatriptan at 0.64±0.01.
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Figure 0001: A typical HPTLC Chromatogram of SumatriptanHPTLC chromatogram represents peak of sumatriptan at 0.64±0.01.

Mentions: A solvent system that would give dense and compact spot with significant Rf value was desired for the quantification of sumatriptan in pharmaceutical formulations. The mobile phase consisting of methanol:water:glacial acetic acid (4.0:8.0:0.1, v/v/v) gave Rf value of 0.64±0.01 for sumatriptan (fig. 1). The linear regression data (n=5, Table 1) showed a good linear relationship over a concentration range of 200-800 ng/spot for sumatriptan. The limit of detection and limit of quantification for sumatriptan were found to be 63.87 and 193.54 ng/spot.


Development and Validation of a HPTLC Method for the Estimation of Sumatriptan in Tablet Dosage Forms.

Shah CR, Suhagia BN, Shah NJ, Shah RR - Indian J Pharm Sci (2008)

A typical HPTLC Chromatogram of SumatriptanHPTLC chromatogram represents peak of sumatriptan at 0.64±0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040890&req=5

Figure 0001: A typical HPTLC Chromatogram of SumatriptanHPTLC chromatogram represents peak of sumatriptan at 0.64±0.01.
Mentions: A solvent system that would give dense and compact spot with significant Rf value was desired for the quantification of sumatriptan in pharmaceutical formulations. The mobile phase consisting of methanol:water:glacial acetic acid (4.0:8.0:0.1, v/v/v) gave Rf value of 0.64±0.01 for sumatriptan (fig. 1). The linear regression data (n=5, Table 1) showed a good linear relationship over a concentration range of 200-800 ng/spot for sumatriptan. The limit of detection and limit of quantification for sumatriptan were found to be 63.87 and 193.54 ng/spot.

Bottom Line: The stationary phase used was precoated silica gel 60F254.The mobile phase used was a mixture of methanol:water:glacial acetic acid (4.0:8.0:0.1, v/v/v).The limit of detection and the limit of quantification for the sumatriptan were found to be 63.87 and 193.54 ng/spot, respectively.

View Article: PubMed Central - PubMed

Affiliation: Shri B. M. Shah College of Pharmaceutical Education and Research, Modasa-383 315, India.

ABSTRACT
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of sumatriptan in tablet dosage forms. The stationary phase used was precoated silica gel 60F254. The mobile phase used was a mixture of methanol:water:glacial acetic acid (4.0:8.0:0.1, v/v/v). The detection of spots was carried out at 230 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 800 ng/spot. The limit of detection and the limit of quantification for the sumatriptan were found to be 63.87 and 193.54 ng/spot, respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.

No MeSH data available.


Related in: MedlinePlus