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Study of the soil isolates for antimicrobial activity.

Srividya AR, Saritha GS, Suresh B - Indian J Pharm Sci (2008)

Bottom Line: That organism was name as NK(2).The antimicrobial compound was extracted in n-butanol, ethyl acetate and methanol.Antimicrobial compound, which was produced by the soil isolate NK(2) did not showed cytotoxic activity on Vero cell lines.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biotechnology, J. S. S College of Pharmacy, Ooty-643 001, India.

ABSTRACT
During the process of screening for a potent antimicrobial compound, a new strain was isolated from the soil sample of Thalaikunda village in Ooty, Tamil Nadu. That organism was name as NK(2). It was found to be antagonistic to both bacterial and fungal test organisms. Production of antibiotic was more in a newly formulated broth. Antibiotic production reached maximum at the end of the 70 h of fermentation by stirred flask culture. The antimicrobial compound was extracted in n-butanol, ethyl acetate and methanol. Antimicrobial compound, which was produced by the soil isolate NK(2) did not showed cytotoxic activity on Vero cell lines.

No MeSH data available.


Related in: MedlinePlus

Scanning electron microscopy of NK2Structure of the soil isolate NK2 under 10, 000 times magnification
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Figure 0001: Scanning electron microscopy of NK2Structure of the soil isolate NK2 under 10, 000 times magnification

Mentions: Soil isolate, NK2, was studied for its morphology and its characteristics by agar block method. The morphology was observed under scanning electron microscopy (fig. 1) Carbon utilization studies were performed and based on the new fermentation medium was formulated9. Development of melanin pigment in Wakesman No. 42 medium was evaluated by streaming the soil isolate on the slants followed by incubation at 28° and the results were observed at the interval of 12 h for 4 days and it was recorded10–13.


Study of the soil isolates for antimicrobial activity.

Srividya AR, Saritha GS, Suresh B - Indian J Pharm Sci (2008)

Scanning electron microscopy of NK2Structure of the soil isolate NK2 under 10, 000 times magnification
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040884&req=5

Figure 0001: Scanning electron microscopy of NK2Structure of the soil isolate NK2 under 10, 000 times magnification
Mentions: Soil isolate, NK2, was studied for its morphology and its characteristics by agar block method. The morphology was observed under scanning electron microscopy (fig. 1) Carbon utilization studies were performed and based on the new fermentation medium was formulated9. Development of melanin pigment in Wakesman No. 42 medium was evaluated by streaming the soil isolate on the slants followed by incubation at 28° and the results were observed at the interval of 12 h for 4 days and it was recorded10–13.

Bottom Line: That organism was name as NK(2).The antimicrobial compound was extracted in n-butanol, ethyl acetate and methanol.Antimicrobial compound, which was produced by the soil isolate NK(2) did not showed cytotoxic activity on Vero cell lines.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biotechnology, J. S. S College of Pharmacy, Ooty-643 001, India.

ABSTRACT
During the process of screening for a potent antimicrobial compound, a new strain was isolated from the soil sample of Thalaikunda village in Ooty, Tamil Nadu. That organism was name as NK(2). It was found to be antagonistic to both bacterial and fungal test organisms. Production of antibiotic was more in a newly formulated broth. Antibiotic production reached maximum at the end of the 70 h of fermentation by stirred flask culture. The antimicrobial compound was extracted in n-butanol, ethyl acetate and methanol. Antimicrobial compound, which was produced by the soil isolate NK(2) did not showed cytotoxic activity on Vero cell lines.

No MeSH data available.


Related in: MedlinePlus