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Fingerprinting of Plumbagin in lic>Drosera burmannii Vahl using High Performance Thin Layer Chromatography.

Madhavan V, Basnett H, Kumar AC, Yoganarasimhan SN - Indian J Pharm Sci (2008)

Bottom Line: Seven components have been detected in the alcohol extract.Further, plumbagin, an useful antifertility agent, was also detected by comparison with the reference standard.The aqueous extract revealed two spots with no spot corresponding to plumbagin.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, M. S. Ramaiah College of Pharmacy, Bangalore-560 054, India.

ABSTRACT
HPTLC fingerprinting profile of the alcohol and aqueous extracts of Drosera burmannii is described. Seven components have been detected in the alcohol extract. Further, plumbagin, an useful antifertility agent, was also detected by comparison with the reference standard. The aqueous extract revealed two spots with no spot corresponding to plumbagin.

No MeSH data available.


Related in: MedlinePlus

Overlay spectrum of plumbagin and alcohol extractOverlay spectrum of plumbagin and alcohol extract of Drosera burmannii at a λmax 425 nm
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Figure 0004: Overlay spectrum of plumbagin and alcohol extractOverlay spectrum of plumbagin and alcohol extract of Drosera burmannii at a λmax 425 nm

Mentions: The method is specific for plumbagin in alcohol extract since it resolves the peak of plumbagin in the mobile phase proposed for the alcohol extracts to an Rf of 0.56 in the presence of other components. The specificity was confirmed by overlaying the spectra of plumbagin in reference standard (λmax 425 nm), with the absorption spectrum obtained from the corresponding band in the track of alcohol extract (fig. 4). The aqueous extract revealed spots at Rf 0.15 and 0.24 with no spot corresponding to that of plumbagin reference standard (Rf 0.66). This is due to the hydrophobic nature of plumbagin13. The study is the first report on HPTLC profile of D. burmannii, which reveals components useful for quality evaluation, and standardization of the drug. Further it confirms the presence of plumbagin as marker compound in D. burmannii.


Fingerprinting of Plumbagin in lic>Drosera burmannii Vahl using High Performance Thin Layer Chromatography.

Madhavan V, Basnett H, Kumar AC, Yoganarasimhan SN - Indian J Pharm Sci (2008)

Overlay spectrum of plumbagin and alcohol extractOverlay spectrum of plumbagin and alcohol extract of Drosera burmannii at a λmax 425 nm
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040879&req=5

Figure 0004: Overlay spectrum of plumbagin and alcohol extractOverlay spectrum of plumbagin and alcohol extract of Drosera burmannii at a λmax 425 nm
Mentions: The method is specific for plumbagin in alcohol extract since it resolves the peak of plumbagin in the mobile phase proposed for the alcohol extracts to an Rf of 0.56 in the presence of other components. The specificity was confirmed by overlaying the spectra of plumbagin in reference standard (λmax 425 nm), with the absorption spectrum obtained from the corresponding band in the track of alcohol extract (fig. 4). The aqueous extract revealed spots at Rf 0.15 and 0.24 with no spot corresponding to that of plumbagin reference standard (Rf 0.66). This is due to the hydrophobic nature of plumbagin13. The study is the first report on HPTLC profile of D. burmannii, which reveals components useful for quality evaluation, and standardization of the drug. Further it confirms the presence of plumbagin as marker compound in D. burmannii.

Bottom Line: Seven components have been detected in the alcohol extract.Further, plumbagin, an useful antifertility agent, was also detected by comparison with the reference standard.The aqueous extract revealed two spots with no spot corresponding to plumbagin.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, M. S. Ramaiah College of Pharmacy, Bangalore-560 054, India.

ABSTRACT
HPTLC fingerprinting profile of the alcohol and aqueous extracts of Drosera burmannii is described. Seven components have been detected in the alcohol extract. Further, plumbagin, an useful antifertility agent, was also detected by comparison with the reference standard. The aqueous extract revealed two spots with no spot corresponding to plumbagin.

No MeSH data available.


Related in: MedlinePlus