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Fingerprinting of Plumbagin in lic>Drosera burmannii Vahl using High Performance Thin Layer Chromatography.

Madhavan V, Basnett H, Kumar AC, Yoganarasimhan SN - Indian J Pharm Sci (2008)

Bottom Line: Seven components have been detected in the alcohol extract.Further, plumbagin, an useful antifertility agent, was also detected by comparison with the reference standard.The aqueous extract revealed two spots with no spot corresponding to plumbagin.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, M. S. Ramaiah College of Pharmacy, Bangalore-560 054, India.

ABSTRACT
HPTLC fingerprinting profile of the alcohol and aqueous extracts of Drosera burmannii is described. Seven components have been detected in the alcohol extract. Further, plumbagin, an useful antifertility agent, was also detected by comparison with the reference standard. The aqueous extract revealed two spots with no spot corresponding to plumbagin.

No MeSH data available.


Related in: MedlinePlus

Chromatogram of alcohol extractStd, std1 is the plumbagin standard (254 nm) alc1, alc2, alc 3 and alc 4 are alcohol extract.
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Figure 0003: Chromatogram of alcohol extractStd, std1 is the plumbagin standard (254 nm) alc1, alc2, alc 3 and alc 4 are alcohol extract.

Mentions: Plumbagin reference standard shows an Rf of 0.56 (fig. 1) and 0.66 in the mobile phase adopted for alcohol and aqueous extracts respectively. HPTLC analysis of alcohol and aqueous extracts of D. burmannii revealed different chromatographic profiles. In this study the alcohol extract revealed seven components at Rf 0.12, 0.18, 0.21, 0.24, 0.29, 0.56, 0.81 (figs. 2 and 3). Out of these, the most pronounced spot of maximum area was at Rf 0.56, corresponding to that of marker compound plumbagin. Three other spots at Rf 0.12, 0.18 and 0.21 were also prominent.


Fingerprinting of Plumbagin in lic>Drosera burmannii Vahl using High Performance Thin Layer Chromatography.

Madhavan V, Basnett H, Kumar AC, Yoganarasimhan SN - Indian J Pharm Sci (2008)

Chromatogram of alcohol extractStd, std1 is the plumbagin standard (254 nm) alc1, alc2, alc 3 and alc 4 are alcohol extract.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040879&req=5

Figure 0003: Chromatogram of alcohol extractStd, std1 is the plumbagin standard (254 nm) alc1, alc2, alc 3 and alc 4 are alcohol extract.
Mentions: Plumbagin reference standard shows an Rf of 0.56 (fig. 1) and 0.66 in the mobile phase adopted for alcohol and aqueous extracts respectively. HPTLC analysis of alcohol and aqueous extracts of D. burmannii revealed different chromatographic profiles. In this study the alcohol extract revealed seven components at Rf 0.12, 0.18, 0.21, 0.24, 0.29, 0.56, 0.81 (figs. 2 and 3). Out of these, the most pronounced spot of maximum area was at Rf 0.56, corresponding to that of marker compound plumbagin. Three other spots at Rf 0.12, 0.18 and 0.21 were also prominent.

Bottom Line: Seven components have been detected in the alcohol extract.Further, plumbagin, an useful antifertility agent, was also detected by comparison with the reference standard.The aqueous extract revealed two spots with no spot corresponding to plumbagin.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, M. S. Ramaiah College of Pharmacy, Bangalore-560 054, India.

ABSTRACT
HPTLC fingerprinting profile of the alcohol and aqueous extracts of Drosera burmannii is described. Seven components have been detected in the alcohol extract. Further, plumbagin, an useful antifertility agent, was also detected by comparison with the reference standard. The aqueous extract revealed two spots with no spot corresponding to plumbagin.

No MeSH data available.


Related in: MedlinePlus